Induction of apoptosis in S180 tumour bearing mice by polysaccharide from Lentinus edodes via mitochondria apoptotic pathway
JLNT, a purified polysaccharide with the structure of β-(1→3)-D-glucose main chains and β-(1→6)-D-glucose side chains and with an average molecular weight of 605.4 kDa, was extracted and isolated from the fruitbodies of Lentinus edodes, which has immunoregulatory activity and anti-tumour effects. In...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-05-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464615001334 |
| _version_ | 1818410027759697920 |
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| author | Yu Zhang Qiang Li Yamin Shu Hongjing Wang Ziming Zheng Jinglin Wang Kaiping Wang |
| author_facet | Yu Zhang Qiang Li Yamin Shu Hongjing Wang Ziming Zheng Jinglin Wang Kaiping Wang |
| author_sort | Yu Zhang |
| collection | DOAJ |
| description | JLNT, a purified polysaccharide with the structure of β-(1→3)-D-glucose main chains and β-(1→6)-D-glucose side chains and with an average molecular weight of 605.4 kDa, was extracted and isolated from the fruitbodies of Lentinus edodes, which has immunoregulatory activity and anti-tumour effects. In the present study, we found that JLNT possessed direct anti-tumour effects on MCF-7 and S180 cells and apoptosis -inducing activity in S180 tumour bearing mice. Therefore, we investigated the mechanisms of JLNT-induced apoptosis. Results showed that JLNT increased the expression of p53, enhanced the Bax/Bcl-2 ratios by up-regulating Bax and down-regulating Bcl-2, promoted the expression of Smac, leading to the activation of caspase-3 and finally the initiation of cell apoptosis in S180 tumour bearing mice. Taken together, these findings provide the experimental evidence that JLNT could induce apoptosis of S180 cells via mitochondria pathways. |
| first_indexed | 2024-12-14T10:09:00Z |
| format | Article |
| id | doaj.art-da987befa28d4c7faed762e5e2d25fd7 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T10:09:00Z |
| publishDate | 2015-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-da987befa28d4c7faed762e5e2d25fd72022-12-21T23:07:04ZengElsevierJournal of Functional Foods1756-46462015-05-0115151159Induction of apoptosis in S180 tumour bearing mice by polysaccharide from Lentinus edodes via mitochondria apoptotic pathwayYu Zhang0Qiang Li1Yamin Shu2Hongjing Wang3Ziming Zheng4Jinglin Wang5Kaiping Wang6Union Hospital of Huazhong University of Science and Technology, Department of Pharmacy, No. 1227, Jiefang Road, 430030 Wuhan, ChinaUnion Hospital of Huazhong University of Science and Technology, Department of Pharmacy, No. 1227, Jiefang Road, 430030 Wuhan, ChinaHubei Key Laboratory of Nature Medicinal Chemistry and Resource Evaluation, Tongji Medical College of Pharmacy, Huazhong University of Science and Technology, 430030 Wuhan, ChinaHubei Key Laboratory of Nature Medicinal Chemistry and Resource Evaluation, Tongji Medical College of Pharmacy, Huazhong University of Science and Technology, 430030 Wuhan, ChinaUnion Hospital of Huazhong University of Science and Technology, Department of Pharmacy, No. 1227, Jiefang Road, 430030 Wuhan, ChinaUnion Hospital of Huazhong University of Science and Technology, Department of Pharmacy, No. 1227, Jiefang Road, 430030 Wuhan, ChinaHubei Key Laboratory of Nature Medicinal Chemistry and Resource Evaluation, Tongji Medical College of Pharmacy, Huazhong University of Science and Technology, 430030 Wuhan, China; Corresponding author. Tongji Medical College of Pharmacy of Huazhong University of Science and Technology, 430030 Wuhan, China. Tel.: +86 27 63559222; fax: +86 27 63559222.JLNT, a purified polysaccharide with the structure of β-(1→3)-D-glucose main chains and β-(1→6)-D-glucose side chains and with an average molecular weight of 605.4 kDa, was extracted and isolated from the fruitbodies of Lentinus edodes, which has immunoregulatory activity and anti-tumour effects. In the present study, we found that JLNT possessed direct anti-tumour effects on MCF-7 and S180 cells and apoptosis -inducing activity in S180 tumour bearing mice. Therefore, we investigated the mechanisms of JLNT-induced apoptosis. Results showed that JLNT increased the expression of p53, enhanced the Bax/Bcl-2 ratios by up-regulating Bax and down-regulating Bcl-2, promoted the expression of Smac, leading to the activation of caspase-3 and finally the initiation of cell apoptosis in S180 tumour bearing mice. Taken together, these findings provide the experimental evidence that JLNT could induce apoptosis of S180 cells via mitochondria pathways.http://www.sciencedirect.com/science/article/pii/S1756464615001334PolysaccharideLentinanApoptosisCaspase-3p53Smac |
| spellingShingle | Yu Zhang Qiang Li Yamin Shu Hongjing Wang Ziming Zheng Jinglin Wang Kaiping Wang Induction of apoptosis in S180 tumour bearing mice by polysaccharide from Lentinus edodes via mitochondria apoptotic pathway Journal of Functional Foods Polysaccharide Lentinan Apoptosis Caspase-3 p53 Smac |
| title | Induction of apoptosis in S180 tumour bearing mice by polysaccharide from Lentinus edodes via mitochondria apoptotic pathway |
| title_full | Induction of apoptosis in S180 tumour bearing mice by polysaccharide from Lentinus edodes via mitochondria apoptotic pathway |
| title_fullStr | Induction of apoptosis in S180 tumour bearing mice by polysaccharide from Lentinus edodes via mitochondria apoptotic pathway |
| title_full_unstemmed | Induction of apoptosis in S180 tumour bearing mice by polysaccharide from Lentinus edodes via mitochondria apoptotic pathway |
| title_short | Induction of apoptosis in S180 tumour bearing mice by polysaccharide from Lentinus edodes via mitochondria apoptotic pathway |
| title_sort | induction of apoptosis in s180 tumour bearing mice by polysaccharide from lentinus edodes via mitochondria apoptotic pathway |
| topic | Polysaccharide Lentinan Apoptosis Caspase-3 p53 Smac |
| url | http://www.sciencedirect.com/science/article/pii/S1756464615001334 |
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