Application of the CRISPR/Cas System in Pathogen Detection: A Review
Early and rapid diagnosis of pathogens is important for the prevention and control of epidemic disease. The polymerase chain reaction (PCR) technique requires expensive instrument control, a special test site, complex solution treatment steps and professional operation, which can limit its applicati...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/20/6999 |
| _version_ | 1797470854281428992 |
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| author | Bowei Yuan Congcong Yuan Lulu Li Miao Long Zeliang Chen |
| author_facet | Bowei Yuan Congcong Yuan Lulu Li Miao Long Zeliang Chen |
| author_sort | Bowei Yuan |
| collection | DOAJ |
| description | Early and rapid diagnosis of pathogens is important for the prevention and control of epidemic disease. The polymerase chain reaction (PCR) technique requires expensive instrument control, a special test site, complex solution treatment steps and professional operation, which can limit its application in practice. The pathogen detection method based on the clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated protein (CRISPR/Cas) system is characterized by strong specificity, high sensitivity and convenience for detection, which is more suitable for practical applications. This article first reviews the CRISPR/Cas system, and then introduces the application of the two types of systems represented by Type II (cas9), Type V (cas12a, cas12b, cas14a) and Type VI (cas13a) in pathogen detection. Finally, challenges and prospects are proposed. |
| first_indexed | 2024-03-09T19:42:49Z |
| format | Article |
| id | doaj.art-da9a8e1d71814821b9cd61ca2a58eccb |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T19:42:49Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-da9a8e1d71814821b9cd61ca2a58eccb2023-11-24T01:35:19ZengMDPI AGMolecules1420-30492022-10-012720699910.3390/molecules27206999Application of the CRISPR/Cas System in Pathogen Detection: A ReviewBowei Yuan0Congcong Yuan1Lulu Li2Miao Long3Zeliang Chen4Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaEarly and rapid diagnosis of pathogens is important for the prevention and control of epidemic disease. The polymerase chain reaction (PCR) technique requires expensive instrument control, a special test site, complex solution treatment steps and professional operation, which can limit its application in practice. The pathogen detection method based on the clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated protein (CRISPR/Cas) system is characterized by strong specificity, high sensitivity and convenience for detection, which is more suitable for practical applications. This article first reviews the CRISPR/Cas system, and then introduces the application of the two types of systems represented by Type II (cas9), Type V (cas12a, cas12b, cas14a) and Type VI (cas13a) in pathogen detection. Finally, challenges and prospects are proposed.https://www.mdpi.com/1420-3049/27/20/6999CRISPR/Caspathogen detection |
| spellingShingle | Bowei Yuan Congcong Yuan Lulu Li Miao Long Zeliang Chen Application of the CRISPR/Cas System in Pathogen Detection: A Review Molecules CRISPR/Cas pathogen detection |
| title | Application of the CRISPR/Cas System in Pathogen Detection: A Review |
| title_full | Application of the CRISPR/Cas System in Pathogen Detection: A Review |
| title_fullStr | Application of the CRISPR/Cas System in Pathogen Detection: A Review |
| title_full_unstemmed | Application of the CRISPR/Cas System in Pathogen Detection: A Review |
| title_short | Application of the CRISPR/Cas System in Pathogen Detection: A Review |
| title_sort | application of the crispr cas system in pathogen detection a review |
| topic | CRISPR/Cas pathogen detection |
| url | https://www.mdpi.com/1420-3049/27/20/6999 |
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