The Effect of Co-administration of 4-Methylcatechol and Progesterone on Sciatic Nerve Function and Neurohistological Alterations in Streptozotocin-Induced Diabetic Neuropathy in Rats
Objective: Diabetic neuropathy is the most common complication of diabetes mellitusaffecting the nervous system. In this study, we investigated the in vivo effects of combinedadministration of 4-methylcatechol (4-MC) and progesterone (P) as a potentialtherapeutic tool for sciatic nerve function impr...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2011-01-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/library/upload/article/af_3222643336635735253234644486346233234664Sameni1.pdf |
| Summary: | Objective: Diabetic neuropathy is the most common complication of diabetes mellitusaffecting the nervous system. In this study, we investigated the in vivo effects of combinedadministration of 4-methylcatechol (4-MC) and progesterone (P) as a potentialtherapeutic tool for sciatic nerve function improvement and its role in histomorphologicalalterations in diabetic neuropathy in rats.Materials and Methods: Male adult rats were divided into 3 groups: sham operated control(CO), untreated diabetic (DM) and diabetic treated with progesterone and 4-methylcatechol(DMP4MC) groups. Diabetes was induced by a single dose injection of 55 mg/kg streptozotocin (STZ). Four weeks after the STZ administration, the DMP4MC groupwas treated with P and 4-MC for 6 weeks. Then, following anesthesia, the animals' sciaticnerves were removed and processed for light and transmission electron microscopy(TEM) as well as histological evaluation.Results: Diabetic rats showed a statistically significant reduction in motor nerve conductionvelocity (MNCV), nerve blood flow (NBF), mean myelinated fiber (MF) diameters andmyelin sheath thickness of the sciatic nerve after 10 weeks. In the sciatic nerve of the untreateddiabetic group, endoneurial edema and increased number of myelinated fibers withmyelin abnormalities such as infolding into the axoplasm, irregularity of fibers and alterationin myelin compaction were also observed. Treatment of diabetic rats with a combinationof P and 4-MC significantly increased MNCV and NBF and prevented endoneurial edemaand all myelin abnormalities.Conclusion: Our findings indicated that co-administration of P and 4-MC may preventsciatic nerve dysfunction and histomorphological alterations in experimental diabetic neuropathy. |
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| ISSN: | 2228-5806 2228-5814 |