Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city.
Background: Primary maternal infection with toxoplasmosis during gestation and its transmission to the fetus continue to be the cause of tragic yet preventable disease in offspring. jective: This study was aimed to Comparison between efficiency of these two methods(nested PCR and IgG avidity test) i...
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| Format: | Article |
| Language: | English |
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University of Anbar
2014-12-01
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| Series: | مجلة جامعة الانبار للعلوم الصرفة |
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| Online Access: | https://juaps.uoanbar.edu.iq/article_123986_d41d8cd98f00b204e9800998ecf8427e.pdf |
| _version_ | 1797373302453305344 |
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| author | Saeed Arrak Turkey Sarab Fawzi Al-Ani Raja Jehad Al-hadithi |
| author_facet | Saeed Arrak Turkey Sarab Fawzi Al-Ani Raja Jehad Al-hadithi |
| author_sort | Saeed Arrak Turkey |
| collection | DOAJ |
| description | Background: Primary maternal infection with toxoplasmosis during gestation and its transmission to the fetus continue to be the cause of tragic yet preventable disease in offspring. jective: This study was aimed to Comparison between efficiency of these two methods(nested PCR and IgG avidity test) in detection of acute toxoplasmosis in pregnant women early in the first trimester is of utmost importance in order to offer them early therapy or other interventions to prevent congenital infection of fetuses. Methods: One hundred sixteen blood samples were collected from pregnant women at different ages, and in the first trimester of pregnancy. These women have history of habitual abortion, intrauterine fetal death and congenital anomalies of the fetus. Blood samples were tested for specific anti-Toxoplasma IgG avidity test and detection of B1 gene of T. gondii by nPCR. Results: In this study, it was found out that the rate of Toxoplasmosis by nested PCR 48 cases (41.4%),while by T. IgG avidity test was 76cases(65.5%) (high avidity 54.3% and low avidity11.2%). The detection rate by nested PCR was significantly higher than by T. IgG avidity test. Conclusion: PCR technique is more sensitive and specific than T.IgG avidity tests. |
| first_indexed | 2024-03-08T18:48:28Z |
| format | Article |
| id | doaj.art-da9c4bf425344d8db37eb89b3c14d7ee |
| institution | Directory Open Access Journal |
| issn | 1991-8941 2706-6703 |
| language | English |
| last_indexed | 2024-03-08T18:48:28Z |
| publishDate | 2014-12-01 |
| publisher | University of Anbar |
| record_format | Article |
| series | مجلة جامعة الانبار للعلوم الصرفة |
| spelling | doaj.art-da9c4bf425344d8db37eb89b3c14d7ee2023-12-28T21:57:35ZengUniversity of Anbarمجلة جامعة الانبار للعلوم الصرفة1991-89412706-67032014-12-01831610.37652/juaps.2014.123986123986Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city.Saeed Arrak Turkey0Sarab Fawzi Al-Ani1Raja Jehad Al-hadithi2University of Anbar - College Of Medicine.University of Anbar - College Of Medicine.University of Anbar - College Of Medicine.Background: Primary maternal infection with toxoplasmosis during gestation and its transmission to the fetus continue to be the cause of tragic yet preventable disease in offspring. jective: This study was aimed to Comparison between efficiency of these two methods(nested PCR and IgG avidity test) in detection of acute toxoplasmosis in pregnant women early in the first trimester is of utmost importance in order to offer them early therapy or other interventions to prevent congenital infection of fetuses. Methods: One hundred sixteen blood samples were collected from pregnant women at different ages, and in the first trimester of pregnancy. These women have history of habitual abortion, intrauterine fetal death and congenital anomalies of the fetus. Blood samples were tested for specific anti-Toxoplasma IgG avidity test and detection of B1 gene of T. gondii by nPCR. Results: In this study, it was found out that the rate of Toxoplasmosis by nested PCR 48 cases (41.4%),while by T. IgG avidity test was 76cases(65.5%) (high avidity 54.3% and low avidity11.2%). The detection rate by nested PCR was significantly higher than by T. IgG avidity test. Conclusion: PCR technique is more sensitive and specific than T.IgG avidity tests.https://juaps.uoanbar.edu.iq/article_123986_d41d8cd98f00b204e9800998ecf8427e.pdftoxoplasma gondiinested pcrtoxoplasmosis |
| spellingShingle | Saeed Arrak Turkey Sarab Fawzi Al-Ani Raja Jehad Al-hadithi Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city. مجلة جامعة الانبار للعلوم الصرفة toxoplasma gondii nested pcr toxoplasmosis |
| title | Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city. |
| title_full | Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city. |
| title_fullStr | Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city. |
| title_full_unstemmed | Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city. |
| title_short | Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city. |
| title_sort | comparison between the efficiency of nested pcr analysis and igg avidity test in detection of acute toxoplasma gondii infection in early pregnancy in ramadi city |
| topic | toxoplasma gondii nested pcr toxoplasmosis |
| url | https://juaps.uoanbar.edu.iq/article_123986_d41d8cd98f00b204e9800998ecf8427e.pdf |
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