COVID-19 Salivary Protein Profile: Unravelling Molecular Aspects of SARS-CoV-2 Infection
COVID-19 is the most impacting global pandemic of all time, with over 600 million infected and 6.5 million deaths worldwide, in addition to an unprecedented economic impact. Despite the many advances in scientific knowledge about the disease, much remains to be clarified about the molecular alterati...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/19/5571 |
| _version_ | 1797478730699898880 |
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| author | Eduardo Esteves Vera M. Mendes Bruno Manadas Rafaela Lopes Liliana Bernardino Maria José Correia Marlene Barros Ana Cristina Esteves Nuno Rosa |
| author_facet | Eduardo Esteves Vera M. Mendes Bruno Manadas Rafaela Lopes Liliana Bernardino Maria José Correia Marlene Barros Ana Cristina Esteves Nuno Rosa |
| author_sort | Eduardo Esteves |
| collection | DOAJ |
| description | COVID-19 is the most impacting global pandemic of all time, with over 600 million infected and 6.5 million deaths worldwide, in addition to an unprecedented economic impact. Despite the many advances in scientific knowledge about the disease, much remains to be clarified about the molecular alterations induced by SARS-CoV-2 infection. In this work, we present a hybrid proteomics and in silico interactomics strategy to establish a COVID-19 salivary protein profile. Data are available via ProteomeXchange with identifier PXD036571. The differential proteome was narrowed down by the Partial Least-Squares Discriminant Analysis and enrichment analysis was performed with FunRich. In parallel, OralInt was used to determine interspecies Protein-Protein Interactions between humans and SARS-CoV-2. Five dysregulated biological processes were identified in the COVID-19 proteome profile: Apoptosis, Energy Pathways, Immune Response, Protein Metabolism and Transport. We identified 10 proteins (KLK 11, IMPA2, ANXA7, PLP2, IGLV2-11, IGHV3-43D, IGKV2-24, TMEM165, VSIG10 and PHB2) that had never been associated with SARS-CoV-2 infection, representing new evidence of the impact of COVID-19. Interactomics analysis showed viral influence on the host immune response, mainly through interaction with the degranulation of neutrophils. The virus alters the host’s energy metabolism and interferes with apoptosis mechanisms. |
| first_indexed | 2024-03-09T21:35:46Z |
| format | Article |
| id | doaj.art-daa059fd26de4658bcff99167a678959 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T21:35:46Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-daa059fd26de4658bcff99167a6789592023-11-23T20:45:16ZengMDPI AGJournal of Clinical Medicine2077-03832022-09-011119557110.3390/jcm11195571COVID-19 Salivary Protein Profile: Unravelling Molecular Aspects of SARS-CoV-2 InfectionEduardo Esteves0Vera M. Mendes1Bruno Manadas2Rafaela Lopes3Liliana Bernardino4Maria José Correia5Marlene Barros6Ana Cristina Esteves7Nuno Rosa8Universidade Católica Portuguesa, Faculty of Dental Medicine (FMD), Center for Interdisciplinary Research in Health (CIIS), 3504-505 Viseu, PortugalCNC—Center for Neuroscience and Cell Biology, CIBB—Centre for Innovative Biomedicine and Biotechnology, University of Coimbra, 3004-504 Coimbra, PortugalCNC—Center for Neuroscience and Cell Biology, CIBB—Centre for Innovative Biomedicine and Biotechnology, University of Coimbra, 3004-504 Coimbra, PortugalUniversidade Católica Portuguesa, Faculty of Dental Medicine (FMD), Center for Interdisciplinary Research in Health (CIIS), 3504-505 Viseu, PortugalHealth Sciences Research Centre (CICS-UBI), Faculty of Health Sciences, University of Beira Interior, 6201-001 Covilhã, PortugalUniversidade Católica Portuguesa, Faculty of Dental Medicine (FMD), Center for Interdisciplinary Research in Health (CIIS), 3504-505 Viseu, PortugalUniversidade Católica Portuguesa, Faculty of Dental Medicine (FMD), Center for Interdisciplinary Research in Health (CIIS), 3504-505 Viseu, PortugalCESAM, Department of Biology, University of Aveiro, 3810-193 Aveiro, PortugalUniversidade Católica Portuguesa, Faculty of Dental Medicine (FMD), Center for Interdisciplinary Research in Health (CIIS), 3504-505 Viseu, PortugalCOVID-19 is the most impacting global pandemic of all time, with over 600 million infected and 6.5 million deaths worldwide, in addition to an unprecedented economic impact. Despite the many advances in scientific knowledge about the disease, much remains to be clarified about the molecular alterations induced by SARS-CoV-2 infection. In this work, we present a hybrid proteomics and in silico interactomics strategy to establish a COVID-19 salivary protein profile. Data are available via ProteomeXchange with identifier PXD036571. The differential proteome was narrowed down by the Partial Least-Squares Discriminant Analysis and enrichment analysis was performed with FunRich. In parallel, OralInt was used to determine interspecies Protein-Protein Interactions between humans and SARS-CoV-2. Five dysregulated biological processes were identified in the COVID-19 proteome profile: Apoptosis, Energy Pathways, Immune Response, Protein Metabolism and Transport. We identified 10 proteins (KLK 11, IMPA2, ANXA7, PLP2, IGLV2-11, IGHV3-43D, IGKV2-24, TMEM165, VSIG10 and PHB2) that had never been associated with SARS-CoV-2 infection, representing new evidence of the impact of COVID-19. Interactomics analysis showed viral influence on the host immune response, mainly through interaction with the degranulation of neutrophils. The virus alters the host’s energy metabolism and interferes with apoptosis mechanisms.https://www.mdpi.com/2077-0383/11/19/5571COVID-19salivaproteomicsinteractomicsOralint |
| spellingShingle | Eduardo Esteves Vera M. Mendes Bruno Manadas Rafaela Lopes Liliana Bernardino Maria José Correia Marlene Barros Ana Cristina Esteves Nuno Rosa COVID-19 Salivary Protein Profile: Unravelling Molecular Aspects of SARS-CoV-2 Infection Journal of Clinical Medicine COVID-19 saliva proteomics interactomics Oralint |
| title | COVID-19 Salivary Protein Profile: Unravelling Molecular Aspects of SARS-CoV-2 Infection |
| title_full | COVID-19 Salivary Protein Profile: Unravelling Molecular Aspects of SARS-CoV-2 Infection |
| title_fullStr | COVID-19 Salivary Protein Profile: Unravelling Molecular Aspects of SARS-CoV-2 Infection |
| title_full_unstemmed | COVID-19 Salivary Protein Profile: Unravelling Molecular Aspects of SARS-CoV-2 Infection |
| title_short | COVID-19 Salivary Protein Profile: Unravelling Molecular Aspects of SARS-CoV-2 Infection |
| title_sort | covid 19 salivary protein profile unravelling molecular aspects of sars cov 2 infection |
| topic | COVID-19 saliva proteomics interactomics Oralint |
| url | https://www.mdpi.com/2077-0383/11/19/5571 |
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