Integrated Transcriptome and Metabolome Analyses Reveal the Anthocyanin Biosynthesis Pathway in AmRosea1 Overexpression 84K Poplar
Populus alba × Populus glandulosa (84K poplar) is model material with excellent genetic engineering resource and ornamental value. In our study, AmRosea1 (Antirrhinum majus) was overexpressed in 84K poplar, and the transgenic 84K (AM) poplar with high content of anthocyanin exhibited red pigmentatio...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.911701/full |
| _version_ | 1811244476783394816 |
|---|---|
| author | Huiling Yan Xinxin Zhang Xiang Li Xuelai Wang Hanxi Li Qiushuang Zhao Peng Yin Ruixue Guo Xiaona Pei Xiaoqing Hu Rui Han Xiyang Zhao |
| author_facet | Huiling Yan Xinxin Zhang Xiang Li Xuelai Wang Hanxi Li Qiushuang Zhao Peng Yin Ruixue Guo Xiaona Pei Xiaoqing Hu Rui Han Xiyang Zhao |
| author_sort | Huiling Yan |
| collection | DOAJ |
| description | Populus alba × Populus glandulosa (84K poplar) is model material with excellent genetic engineering resource and ornamental value. In our study, AmRosea1 (Antirrhinum majus) was overexpressed in 84K poplar, and the transgenic 84K (AM) poplar with high content of anthocyanin exhibited red pigmentation leaves. The transcriptome analysis between wild type (WT) and AM showed that 170 differentially expressed genes (DEGs) (86 up-regulated and 84 down-regulated) were found, and some DEGs were involved in flavone and flavonol biosynthesis, flavonoid biosynthesis and anthocyanin biosynthesis. The metabolome analysis showed that 13 anthocyanins-related differentially accumulated metabolites (DAMs) were detected in AM. The correlation analysis between DEGs and DAMs were performed, and the results revealed that 18 DEGs, including 11 MYB genes, two BZ1 genes, one FG2 gene, one ANS gene, and three IF7MAT genes, were negatively or positively correlated with 13 DAMs. The phylogenetic analysis demonstrated that there was high homology between AmRosea1 and PagMYB113, and MYB113 co-expressed with BZ1, ANS and DFR directly. Our results elucidated the molecular mechanism of plant color change mediated by anthocyanin biosynthesis pathway, which laid the foundation for the development and utilization of colorful woody plant. |
| first_indexed | 2024-04-12T14:24:37Z |
| format | Article |
| id | doaj.art-daa2007c0f2941608d4547dfe45aad30 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-12T14:24:37Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-daa2007c0f2941608d4547dfe45aad302022-12-22T03:29:28ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-06-011010.3389/fbioe.2022.911701911701Integrated Transcriptome and Metabolome Analyses Reveal the Anthocyanin Biosynthesis Pathway in AmRosea1 Overexpression 84K PoplarHuiling YanXinxin ZhangXiang LiXuelai WangHanxi LiQiushuang ZhaoPeng YinRuixue GuoXiaona PeiXiaoqing HuRui HanXiyang ZhaoPopulus alba × Populus glandulosa (84K poplar) is model material with excellent genetic engineering resource and ornamental value. In our study, AmRosea1 (Antirrhinum majus) was overexpressed in 84K poplar, and the transgenic 84K (AM) poplar with high content of anthocyanin exhibited red pigmentation leaves. The transcriptome analysis between wild type (WT) and AM showed that 170 differentially expressed genes (DEGs) (86 up-regulated and 84 down-regulated) were found, and some DEGs were involved in flavone and flavonol biosynthesis, flavonoid biosynthesis and anthocyanin biosynthesis. The metabolome analysis showed that 13 anthocyanins-related differentially accumulated metabolites (DAMs) were detected in AM. The correlation analysis between DEGs and DAMs were performed, and the results revealed that 18 DEGs, including 11 MYB genes, two BZ1 genes, one FG2 gene, one ANS gene, and three IF7MAT genes, were negatively or positively correlated with 13 DAMs. The phylogenetic analysis demonstrated that there was high homology between AmRosea1 and PagMYB113, and MYB113 co-expressed with BZ1, ANS and DFR directly. Our results elucidated the molecular mechanism of plant color change mediated by anthocyanin biosynthesis pathway, which laid the foundation for the development and utilization of colorful woody plant.https://www.frontiersin.org/articles/10.3389/fbioe.2022.911701/fullPopulus alba × Populus glandulosa (84K poplar)anthocyaninAmRosea1metabolometranscriptome |
| spellingShingle | Huiling Yan Xinxin Zhang Xiang Li Xuelai Wang Hanxi Li Qiushuang Zhao Peng Yin Ruixue Guo Xiaona Pei Xiaoqing Hu Rui Han Xiyang Zhao Integrated Transcriptome and Metabolome Analyses Reveal the Anthocyanin Biosynthesis Pathway in AmRosea1 Overexpression 84K Poplar Frontiers in Bioengineering and Biotechnology Populus alba × Populus glandulosa (84K poplar) anthocyanin AmRosea1 metabolome transcriptome |
| title | Integrated Transcriptome and Metabolome Analyses Reveal the Anthocyanin Biosynthesis Pathway in AmRosea1 Overexpression 84K Poplar |
| title_full | Integrated Transcriptome and Metabolome Analyses Reveal the Anthocyanin Biosynthesis Pathway in AmRosea1 Overexpression 84K Poplar |
| title_fullStr | Integrated Transcriptome and Metabolome Analyses Reveal the Anthocyanin Biosynthesis Pathway in AmRosea1 Overexpression 84K Poplar |
| title_full_unstemmed | Integrated Transcriptome and Metabolome Analyses Reveal the Anthocyanin Biosynthesis Pathway in AmRosea1 Overexpression 84K Poplar |
| title_short | Integrated Transcriptome and Metabolome Analyses Reveal the Anthocyanin Biosynthesis Pathway in AmRosea1 Overexpression 84K Poplar |
| title_sort | integrated transcriptome and metabolome analyses reveal the anthocyanin biosynthesis pathway in amrosea1 overexpression 84k poplar |
| topic | Populus alba × Populus glandulosa (84K poplar) anthocyanin AmRosea1 metabolome transcriptome |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.911701/full |
| work_keys_str_mv | AT huilingyan integratedtranscriptomeandmetabolomeanalysesrevealtheanthocyaninbiosynthesispathwayinamrosea1overexpression84kpoplar AT xinxinzhang integratedtranscriptomeandmetabolomeanalysesrevealtheanthocyaninbiosynthesispathwayinamrosea1overexpression84kpoplar AT xiangli integratedtranscriptomeandmetabolomeanalysesrevealtheanthocyaninbiosynthesispathwayinamrosea1overexpression84kpoplar AT xuelaiwang integratedtranscriptomeandmetabolomeanalysesrevealtheanthocyaninbiosynthesispathwayinamrosea1overexpression84kpoplar AT hanxili integratedtranscriptomeandmetabolomeanalysesrevealtheanthocyaninbiosynthesispathwayinamrosea1overexpression84kpoplar AT qiushuangzhao integratedtranscriptomeandmetabolomeanalysesrevealtheanthocyaninbiosynthesispathwayinamrosea1overexpression84kpoplar AT pengyin integratedtranscriptomeandmetabolomeanalysesrevealtheanthocyaninbiosynthesispathwayinamrosea1overexpression84kpoplar AT ruixueguo integratedtranscriptomeandmetabolomeanalysesrevealtheanthocyaninbiosynthesispathwayinamrosea1overexpression84kpoplar AT xiaonapei integratedtranscriptomeandmetabolomeanalysesrevealtheanthocyaninbiosynthesispathwayinamrosea1overexpression84kpoplar AT xiaoqinghu integratedtranscriptomeandmetabolomeanalysesrevealtheanthocyaninbiosynthesispathwayinamrosea1overexpression84kpoplar AT ruihan integratedtranscriptomeandmetabolomeanalysesrevealtheanthocyaninbiosynthesispathwayinamrosea1overexpression84kpoplar AT xiyangzhao integratedtranscriptomeandmetabolomeanalysesrevealtheanthocyaninbiosynthesispathwayinamrosea1overexpression84kpoplar |