Combining High-Resolution Hard X-ray Tomography and Histology for Stem Cell-Mediated Distraction Osteogenesis
Distraction osteogenesis is a clinically established technique for lengthening, molding and shaping bone by new bone formation. The experimental evaluation of this expensive and time-consuming treatment is of high impact for better understanding of tissue engineering but mainly relies on a limited n...
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MDPI AG
2022-06-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/12/6286 |
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| author | Griffin Rodgers Guido R. Sigron Christine Tanner Simone E. Hieber Felix Beckmann Georg Schulz Arnaud Scherberich Claude Jaquiéry Christoph Kunz Bert Müller |
| author_facet | Griffin Rodgers Guido R. Sigron Christine Tanner Simone E. Hieber Felix Beckmann Georg Schulz Arnaud Scherberich Claude Jaquiéry Christoph Kunz Bert Müller |
| author_sort | Griffin Rodgers |
| collection | DOAJ |
| description | Distraction osteogenesis is a clinically established technique for lengthening, molding and shaping bone by new bone formation. The experimental evaluation of this expensive and time-consuming treatment is of high impact for better understanding of tissue engineering but mainly relies on a limited number of histological slices. These tissue slices contain two-dimensional information comprising only about one percent of the volume of interest. In order to analyze the soft and hard tissues of the entire jaw of a single rat in a multimodal assessment, we combined micro computed tomography (µCT) with histology. The µCT data acquired before and after decalcification were registered to determine the impact of decalcification on local tissue shrinkage. Identification of the location of the H&E-stained specimen within the synchrotron radiation-based µCT data collected after decalcification was achieved via non-rigid slice-to-volume registration. The resulting bi- and tri-variate histograms were divided into clusters related to anatomical features from bone and soft tissues, which allowed for a comparison of the approaches and resulted in the hypothesis that the combination of laboratory-based µCT before decalcification, synchrotron radiation-based µCT after decalcification and histology with hematoxylin-and-eosin staining could be used to discriminate between different types of collagen, key components of new bone formation. |
| first_indexed | 2024-03-10T00:28:04Z |
| format | Article |
| id | doaj.art-daa280db3c254e0abd3ca7e6056af384 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T00:28:04Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-daa280db3c254e0abd3ca7e6056af3842023-11-23T15:30:58ZengMDPI AGApplied Sciences2076-34172022-06-011212628610.3390/app12126286Combining High-Resolution Hard X-ray Tomography and Histology for Stem Cell-Mediated Distraction OsteogenesisGriffin Rodgers0Guido R. Sigron1Christine Tanner2Simone E. Hieber3Felix Beckmann4Georg Schulz5Arnaud Scherberich6Claude Jaquiéry7Christoph Kunz8Bert Müller9Biomaterials Science Center, Department of Biomedical Engineering, University of Basel, 4123 Allschwil, SwitzerlandBiomaterials Science Center, Department of Biomedical Engineering, University of Basel, 4123 Allschwil, SwitzerlandBiomaterials Science Center, Department of Biomedical Engineering, University of Basel, 4123 Allschwil, SwitzerlandBiomaterials Science Center, Department of Biomedical Engineering, University of Basel, 4123 Allschwil, SwitzerlandInstitute of Materials Physics, Helmholtz-Zentrum Hereon, 21502 Geesthacht, GermanyBiomaterials Science Center, Department of Biomedical Engineering, University of Basel, 4123 Allschwil, SwitzerlandVascularized Bone Biofabrication, Department of Biomedical Engineering, University of Basel, 4123 Allschwil, SwitzerlandClinic of Oral and Cranio-Maxillofacial Surgery, Department of Surgery, University Hospital of Basel, 4031 Basel, SwitzerlandClinic of Oral and Cranio-Maxillofacial Surgery, Department of Surgery, University Hospital of Basel, 4031 Basel, SwitzerlandBiomaterials Science Center, Department of Biomedical Engineering, University of Basel, 4123 Allschwil, SwitzerlandDistraction osteogenesis is a clinically established technique for lengthening, molding and shaping bone by new bone formation. The experimental evaluation of this expensive and time-consuming treatment is of high impact for better understanding of tissue engineering but mainly relies on a limited number of histological slices. These tissue slices contain two-dimensional information comprising only about one percent of the volume of interest. In order to analyze the soft and hard tissues of the entire jaw of a single rat in a multimodal assessment, we combined micro computed tomography (µCT) with histology. The µCT data acquired before and after decalcification were registered to determine the impact of decalcification on local tissue shrinkage. Identification of the location of the H&E-stained specimen within the synchrotron radiation-based µCT data collected after decalcification was achieved via non-rigid slice-to-volume registration. The resulting bi- and tri-variate histograms were divided into clusters related to anatomical features from bone and soft tissues, which allowed for a comparison of the approaches and resulted in the hypothesis that the combination of laboratory-based µCT before decalcification, synchrotron radiation-based µCT after decalcification and histology with hematoxylin-and-eosin staining could be used to discriminate between different types of collagen, key components of new bone formation.https://www.mdpi.com/2076-3417/12/12/6286distraction osteogenesis in oral and maxillofacial surgeryrat modelhard X-ray tomographysynchrotron radiationnon-rigid 2D-3D registrationjoint histogram |
| spellingShingle | Griffin Rodgers Guido R. Sigron Christine Tanner Simone E. Hieber Felix Beckmann Georg Schulz Arnaud Scherberich Claude Jaquiéry Christoph Kunz Bert Müller Combining High-Resolution Hard X-ray Tomography and Histology for Stem Cell-Mediated Distraction Osteogenesis Applied Sciences distraction osteogenesis in oral and maxillofacial surgery rat model hard X-ray tomography synchrotron radiation non-rigid 2D-3D registration joint histogram |
| title | Combining High-Resolution Hard X-ray Tomography and Histology for Stem Cell-Mediated Distraction Osteogenesis |
| title_full | Combining High-Resolution Hard X-ray Tomography and Histology for Stem Cell-Mediated Distraction Osteogenesis |
| title_fullStr | Combining High-Resolution Hard X-ray Tomography and Histology for Stem Cell-Mediated Distraction Osteogenesis |
| title_full_unstemmed | Combining High-Resolution Hard X-ray Tomography and Histology for Stem Cell-Mediated Distraction Osteogenesis |
| title_short | Combining High-Resolution Hard X-ray Tomography and Histology for Stem Cell-Mediated Distraction Osteogenesis |
| title_sort | combining high resolution hard x ray tomography and histology for stem cell mediated distraction osteogenesis |
| topic | distraction osteogenesis in oral and maxillofacial surgery rat model hard X-ray tomography synchrotron radiation non-rigid 2D-3D registration joint histogram |
| url | https://www.mdpi.com/2076-3417/12/12/6286 |
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