Lectins use for receive and study of glioma C6 cells with stem cells properties
The purpose — to enrich suspension of glioma C6 cells in rats with stem tumor stem cells using lectins sorbed on plastic.Materials and methods. Lectins of beans (Phaseolus vulgaris — PHA), concanavalin (ConA), peanuts (peanut agglutinin — PNA), mistletoe (Mil), labrum anagyroides agglutin...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Romodanov Neurosurgery Institute
2015-06-01
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| Series: | Ukrainian Neurosurgical Journal |
| Online Access: | https://theunj.org/article/view/45289 |
| _version_ | 1797348317978427392 |
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| author | Nikolay Lisianyi Irina Gnedkova Diana Stanetskaya Volodymyr Rozumenko Alexandr Glavatskiy Anna Shmeleva Tatyana Malysheva Oksana Chernenko Mаrianna Gnedkova |
| author_facet | Nikolay Lisianyi Irina Gnedkova Diana Stanetskaya Volodymyr Rozumenko Alexandr Glavatskiy Anna Shmeleva Tatyana Malysheva Oksana Chernenko Mаrianna Gnedkova |
| author_sort | Nikolay Lisianyi |
| collection | DOAJ |
| description |
The purpose — to enrich suspension of glioma C6 cells in rats with stem tumor stem cells using lectins sorbed on plastic.Materials and methods. Lectins of beans (Phaseolus vulgaris — PHA), concanavalin (ConA), peanuts (peanut agglutinin — PNA), mistletoe (Mil), labrum anagyroides agglutinin (LABA), soybean (SBA) were used to separate glioma C6 cells.Glioma C6 cells were cultivated with sorbed lectins for 24 h and were injected into the brain of the rats.Results. Tumorogenic activity was significantly higher in fractions of cells adhered to plastic with adsorbed lectins PHA, ConA, PNA, LABA and SBA, that was an indirect evidence of these fractions enriched by tumor stem cells. Lectin Mil exert an antitumor effect that contributed to rats’ lifetime increasing.Conclusions. The results of the study indicate the possibility of tumor cells division using lectins. |
| first_indexed | 2024-03-08T12:04:12Z |
| format | Article |
| id | doaj.art-daa3a9c90ce84e3f83a0636bae1b9daa |
| institution | Directory Open Access Journal |
| issn | 2663-9084 2663-9092 |
| language | English |
| last_indexed | 2024-03-08T12:04:12Z |
| publishDate | 2015-06-01 |
| publisher | Romodanov Neurosurgery Institute |
| record_format | Article |
| series | Ukrainian Neurosurgical Journal |
| spelling | doaj.art-daa3a9c90ce84e3f83a0636bae1b9daa2024-01-23T13:31:37ZengRomodanov Neurosurgery InstituteUkrainian Neurosurgical Journal2663-90842663-90922015-06-012111610.25305/unj.45289Lectins use for receive and study of glioma C6 cells with stem cells propertiesNikolay Lisianyi0Irina Gnedkova1Diana Stanetskaya2Volodymyr Rozumenko3Alexandr Glavatskiy4Anna Shmeleva5Tatyana Malysheva6Oksana Chernenko7Mаrianna Gnedkova8Romodanov Neurosurgery Institute, Kiev, UkraineNeuroimmunology Department, Romodanov Neurosurgery Institute, Kiev, UkraineNeuroimmunology Department, Romodanov Neurosurgery Institute, Kiev, UkraineIntracerebral Tumors Department, Romodanov Neurosurgery Institute, Kiev, UkraineIntracerebral Tumors Department, Romodanov Neurosurgery Institute, Kiev, UkraineNeuropathomorphology Department, Romodanov Neurosurgery Institute, Kiev, UkraineNeuropathomorphology Department, Romodanov Neurosurgery Institute, Kiev, UkraineNeuropathomorphology Department, Romodanov Neurosurgery Institute, Kiev, UkraineDepartment of Anesthesiology and Intensive Care #2, Romodanov Neurosurgery Institute, Kiev, Ukraine The purpose — to enrich suspension of glioma C6 cells in rats with stem tumor stem cells using lectins sorbed on plastic.Materials and methods. Lectins of beans (Phaseolus vulgaris — PHA), concanavalin (ConA), peanuts (peanut agglutinin — PNA), mistletoe (Mil), labrum anagyroides agglutinin (LABA), soybean (SBA) were used to separate glioma C6 cells.Glioma C6 cells were cultivated with sorbed lectins for 24 h and were injected into the brain of the rats.Results. Tumorogenic activity was significantly higher in fractions of cells adhered to plastic with adsorbed lectins PHA, ConA, PNA, LABA and SBA, that was an indirect evidence of these fractions enriched by tumor stem cells. Lectin Mil exert an antitumor effect that contributed to rats’ lifetime increasing.Conclusions. The results of the study indicate the possibility of tumor cells division using lectins.https://theunj.org/article/view/45289 |
| spellingShingle | Nikolay Lisianyi Irina Gnedkova Diana Stanetskaya Volodymyr Rozumenko Alexandr Glavatskiy Anna Shmeleva Tatyana Malysheva Oksana Chernenko Mаrianna Gnedkova Lectins use for receive and study of glioma C6 cells with stem cells properties Ukrainian Neurosurgical Journal |
| title | Lectins use for receive and study of glioma C6 cells with stem cells properties |
| title_full | Lectins use for receive and study of glioma C6 cells with stem cells properties |
| title_fullStr | Lectins use for receive and study of glioma C6 cells with stem cells properties |
| title_full_unstemmed | Lectins use for receive and study of glioma C6 cells with stem cells properties |
| title_short | Lectins use for receive and study of glioma C6 cells with stem cells properties |
| title_sort | lectins use for receive and study of glioma c6 cells with stem cells properties |
| url | https://theunj.org/article/view/45289 |
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