The Landscape of the Tumor-Infiltrating Immune Cell and Prognostic Nomogram in Colorectal Cancer
Tumor-infiltrating immune cells are associated with prognosis and immunotherapy targets in colorectal cancer (CRC). The recently developed CIBERSORT method allows immune cell analysis by deconvolution of high-throughput data onto gene expression. In this study, we analyzed the relative proportions o...
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| Format: | Article |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.891270/full |
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| author | Jiateng Zhong Jiateng Zhong Jiateng Zhong Jiateng Zhong Yu Qin Yu Qin Pei Yu Weiyue Xia Baoru Gu Xinlai Qian Yuhan Hu Wei Su Zheying Zhang Zheying Zhang |
| author_facet | Jiateng Zhong Jiateng Zhong Jiateng Zhong Jiateng Zhong Yu Qin Yu Qin Pei Yu Weiyue Xia Baoru Gu Xinlai Qian Yuhan Hu Wei Su Zheying Zhang Zheying Zhang |
| author_sort | Jiateng Zhong |
| collection | DOAJ |
| description | Tumor-infiltrating immune cells are associated with prognosis and immunotherapy targets in colorectal cancer (CRC). The recently developed CIBERSORT method allows immune cell analysis by deconvolution of high-throughput data onto gene expression. In this study, we analyzed the relative proportions of immune cells in GEO (94 samples) and TCGA (522 samples) CRC data based on the CIBERSORT method. A total of 22 types of tumor-infiltrating immune cells were evaluated. Combined with GEO and TCGA data, it was found that naive B cells, M2 macrophages, and resting mast cells were highly expressed in normal tissues, while M0 macrophages, M1 macrophages, activated mast cells, and neutrophils were highly expressed in tumors. Moreover, we constructed a prognostic model by infiltrating immune cells that showed high specificity and sensitivity in both the training (AUC of 5-year survival = 0.699) and validation (AUC of 5-year survival = 0.844) sets. This provides another basis for clinical prognosis. The results of multiple immunofluorescence detection showed that there were differences in the results of bioinformatics analysis. Neutrophils were highly expressed in normal tissues, and M2 macrophages were highly expressed in tumor tissues. Collectively, our data suggested that infiltrating immune cells in CRC may be an important determinant of prognosis and immunotherapy. |
| first_indexed | 2024-12-12T05:26:03Z |
| format | Article |
| id | doaj.art-daa4af62af134701beaec6fea0dde1ac |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-12T05:26:03Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-daa4af62af134701beaec6fea0dde1ac2022-12-22T00:36:27ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-05-011310.3389/fgene.2022.891270891270The Landscape of the Tumor-Infiltrating Immune Cell and Prognostic Nomogram in Colorectal CancerJiateng Zhong0Jiateng Zhong1Jiateng Zhong2Jiateng Zhong3Yu Qin4Yu Qin5Pei Yu6Weiyue Xia7Baoru Gu8Xinlai Qian9Yuhan Hu10Wei Su11Zheying Zhang12Zheying Zhang13Department of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, Xinxiang Medical University, Xinxiang, ChinaDepartment of Gynecology, The Fourth Clinical College of Xinxiang Medical University, Xinxiang, ChinaXinxiang Key Laboratory of Tumor Vaccine and Immunotherapy, Institute of Precision Medicine, Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, Xinxiang Medical University, Xinxiang, ChinaTumor-infiltrating immune cells are associated with prognosis and immunotherapy targets in colorectal cancer (CRC). The recently developed CIBERSORT method allows immune cell analysis by deconvolution of high-throughput data onto gene expression. In this study, we analyzed the relative proportions of immune cells in GEO (94 samples) and TCGA (522 samples) CRC data based on the CIBERSORT method. A total of 22 types of tumor-infiltrating immune cells were evaluated. Combined with GEO and TCGA data, it was found that naive B cells, M2 macrophages, and resting mast cells were highly expressed in normal tissues, while M0 macrophages, M1 macrophages, activated mast cells, and neutrophils were highly expressed in tumors. Moreover, we constructed a prognostic model by infiltrating immune cells that showed high specificity and sensitivity in both the training (AUC of 5-year survival = 0.699) and validation (AUC of 5-year survival = 0.844) sets. This provides another basis for clinical prognosis. The results of multiple immunofluorescence detection showed that there were differences in the results of bioinformatics analysis. Neutrophils were highly expressed in normal tissues, and M2 macrophages were highly expressed in tumor tissues. Collectively, our data suggested that infiltrating immune cells in CRC may be an important determinant of prognosis and immunotherapy.https://www.frontiersin.org/articles/10.3389/fgene.2022.891270/fullCIBERSORTimmunotherapycolorectal cancerprognosisnomogram |
| spellingShingle | Jiateng Zhong Jiateng Zhong Jiateng Zhong Jiateng Zhong Yu Qin Yu Qin Pei Yu Weiyue Xia Baoru Gu Xinlai Qian Yuhan Hu Wei Su Zheying Zhang Zheying Zhang The Landscape of the Tumor-Infiltrating Immune Cell and Prognostic Nomogram in Colorectal Cancer Frontiers in Genetics CIBERSORT immunotherapy colorectal cancer prognosis nomogram |
| title | The Landscape of the Tumor-Infiltrating Immune Cell and Prognostic Nomogram in Colorectal Cancer |
| title_full | The Landscape of the Tumor-Infiltrating Immune Cell and Prognostic Nomogram in Colorectal Cancer |
| title_fullStr | The Landscape of the Tumor-Infiltrating Immune Cell and Prognostic Nomogram in Colorectal Cancer |
| title_full_unstemmed | The Landscape of the Tumor-Infiltrating Immune Cell and Prognostic Nomogram in Colorectal Cancer |
| title_short | The Landscape of the Tumor-Infiltrating Immune Cell and Prognostic Nomogram in Colorectal Cancer |
| title_sort | landscape of the tumor infiltrating immune cell and prognostic nomogram in colorectal cancer |
| topic | CIBERSORT immunotherapy colorectal cancer prognosis nomogram |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.891270/full |
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