A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging
Imaging is used to map activity across populations of neurons. Microscopes with cellular resolution have small (<1 millimeter) fields of view and cannot simultaneously image activity distributed across multiple brain areas. Typical large field of view microscopes do not resolve single cells,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-06-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/14472 |
| _version_ | 1811200204138872832 |
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| author | Nicholas James Sofroniew Daniel Flickinger Jonathan King Karel Svoboda |
| author_facet | Nicholas James Sofroniew Daniel Flickinger Jonathan King Karel Svoboda |
| author_sort | Nicholas James Sofroniew |
| collection | DOAJ |
| description | Imaging is used to map activity across populations of neurons. Microscopes with cellular resolution have small (<1 millimeter) fields of view and cannot simultaneously image activity distributed across multiple brain areas. Typical large field of view microscopes do not resolve single cells, especially in the axial dimension. We developed a 2-photon random access mesoscope (2p-RAM) that allows high-resolution imaging anywhere within a volume spanning multiple brain areas (∅ 5 mm x 1 mm cylinder). 2p-RAM resolution is near diffraction limited (lateral, 0.66 μm, axial 4.09 μm at the center; excitation wavelength = 970 nm; numerical aperture = 0.6) over a large range of excitation wavelengths. A fast three-dimensional scanning system allows efficient sampling of neural activity in arbitrary regions of interest across the entire imaging volume. We illustrate the use of the 2p-RAM by imaging neural activity in multiple, non-contiguous brain areas in transgenic mice expressing protein calcium sensors. |
| first_indexed | 2024-04-12T01:59:42Z |
| format | Article |
| id | doaj.art-daa5abcaf83347018a5c04b8a11dfe0b |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:59:42Z |
| publishDate | 2016-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-daa5abcaf83347018a5c04b8a11dfe0b2022-12-22T03:52:42ZengeLife Sciences Publications LtdeLife2050-084X2016-06-01510.7554/eLife.14472A large field of view two-photon mesoscope with subcellular resolution for in vivo imagingNicholas James Sofroniew0Daniel Flickinger1https://orcid.org/0000-0002-8361-3122Jonathan King2Karel Svoboda3https://orcid.org/0000-0002-6670-7362Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesVidrio Technologies, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesImaging is used to map activity across populations of neurons. Microscopes with cellular resolution have small (<1 millimeter) fields of view and cannot simultaneously image activity distributed across multiple brain areas. Typical large field of view microscopes do not resolve single cells, especially in the axial dimension. We developed a 2-photon random access mesoscope (2p-RAM) that allows high-resolution imaging anywhere within a volume spanning multiple brain areas (∅ 5 mm x 1 mm cylinder). 2p-RAM resolution is near diffraction limited (lateral, 0.66 μm, axial 4.09 μm at the center; excitation wavelength = 970 nm; numerical aperture = 0.6) over a large range of excitation wavelengths. A fast three-dimensional scanning system allows efficient sampling of neural activity in arbitrary regions of interest across the entire imaging volume. We illustrate the use of the 2p-RAM by imaging neural activity in multiple, non-contiguous brain areas in transgenic mice expressing protein calcium sensors.https://elifesciences.org/articles/14472calcium2-photontwo-photonbarrel cortexdendritic spinesneural coding |
| spellingShingle | Nicholas James Sofroniew Daniel Flickinger Jonathan King Karel Svoboda A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging eLife calcium 2-photon two-photon barrel cortex dendritic spines neural coding |
| title | A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging |
| title_full | A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging |
| title_fullStr | A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging |
| title_full_unstemmed | A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging |
| title_short | A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging |
| title_sort | large field of view two photon mesoscope with subcellular resolution for in vivo imaging |
| topic | calcium 2-photon two-photon barrel cortex dendritic spines neural coding |
| url | https://elifesciences.org/articles/14472 |
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