In-Vivo Biophoton Emission, Physiological and Oxidative Responses of Biostimulant-Treated Winter Wheat (<i>Triticum eastivum</i> L.) as Seed Priming Possibility, for Heat Stress Alleviation
High temperature induces oxidative processes in wheat, the alleviation of which is promising using biostimulants. Priming has been used for enhancing stress tolerance of seedlings. However, the usage of biostimulants for priming is an unexplored area under either normal or stress conditions. Therefo...
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MDPI AG
2022-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/5/640 |
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| author | Ildikó Jócsák Henrik Gyalog Richárd Hoffmann Katalin Somfalvi-Tóth |
| author_facet | Ildikó Jócsák Henrik Gyalog Richárd Hoffmann Katalin Somfalvi-Tóth |
| author_sort | Ildikó Jócsák |
| collection | DOAJ |
| description | High temperature induces oxidative processes in wheat, the alleviation of which is promising using biostimulants. Priming has been used for enhancing stress tolerance of seedlings. However, the usage of biostimulants for priming is an unexplored area under either normal or stress conditions. Therefore, the aim of our study was to evaluate the heat stress alleviation capability of differentially applied biostimulant treatments on wheat seedlings. The investigation included stress parameters (fresh/dry weight ratio, chlorophyll content estimation, antioxidant capacity and lipid oxidation) combined with biophoton emission measurement, since with this latter non-invasive technique, it is possible to measure and elucidate in vivo stress conditions in real-time using lipid oxidation-related photon emissions. We confirmed that a single biostimulant pretreatment increased antioxidant capacity and decreased biophoton release and lipid oxidation, indicating the reduction of the harmful effects of heat stress. Therefore, biophoton emission proved to be suitable for detecting and imaging the effects of heat stress on wheat seedlings for the first time. Two-way analysis of variance (ANOVA) revealed that biostimulant (<i>p</i> = 4.01 × 10<sup>−7</sup>) treatments, temperature (<i>p</i> = 9.07 × 10<sup>−8</sup>), and the interaction of the two factors (<i>p</i> = 2.07 × 10<sup>−5</sup>) had a significant effect on the overall count per second values of biophoton emission, predicting more efficient biostimulant utilization practices, even for seed priming purposes. |
| first_indexed | 2024-03-09T20:26:37Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T20:26:37Z |
| publishDate | 2022-02-01 |
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| series | Plants |
| spelling | doaj.art-daa667bf796e4accaedc63c65e698fd72023-11-23T23:35:55ZengMDPI AGPlants2223-77472022-02-0111564010.3390/plants11050640In-Vivo Biophoton Emission, Physiological and Oxidative Responses of Biostimulant-Treated Winter Wheat (<i>Triticum eastivum</i> L.) as Seed Priming Possibility, for Heat Stress AlleviationIldikó Jócsák0Henrik Gyalog1Richárd Hoffmann2Katalin Somfalvi-Tóth3Department of Agronomy, Institute of Agronomy, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, Guba Sándor Street 40, H-7400 Kaposvár, HungaryDepartment of Agronomy, Institute of Agronomy, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, Guba Sándor Street 40, H-7400 Kaposvár, HungaryDepartment of Agronomy, Institute of Agronomy, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, Guba Sándor Street 40, H-7400 Kaposvár, HungaryDepartment of Agronomy, Institute of Agronomy, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, Guba Sándor Street 40, H-7400 Kaposvár, HungaryHigh temperature induces oxidative processes in wheat, the alleviation of which is promising using biostimulants. Priming has been used for enhancing stress tolerance of seedlings. However, the usage of biostimulants for priming is an unexplored area under either normal or stress conditions. Therefore, the aim of our study was to evaluate the heat stress alleviation capability of differentially applied biostimulant treatments on wheat seedlings. The investigation included stress parameters (fresh/dry weight ratio, chlorophyll content estimation, antioxidant capacity and lipid oxidation) combined with biophoton emission measurement, since with this latter non-invasive technique, it is possible to measure and elucidate in vivo stress conditions in real-time using lipid oxidation-related photon emissions. We confirmed that a single biostimulant pretreatment increased antioxidant capacity and decreased biophoton release and lipid oxidation, indicating the reduction of the harmful effects of heat stress. Therefore, biophoton emission proved to be suitable for detecting and imaging the effects of heat stress on wheat seedlings for the first time. Two-way analysis of variance (ANOVA) revealed that biostimulant (<i>p</i> = 4.01 × 10<sup>−7</sup>) treatments, temperature (<i>p</i> = 9.07 × 10<sup>−8</sup>), and the interaction of the two factors (<i>p</i> = 2.07 × 10<sup>−5</sup>) had a significant effect on the overall count per second values of biophoton emission, predicting more efficient biostimulant utilization practices, even for seed priming purposes.https://www.mdpi.com/2223-7747/11/5/640wheatheat stressbiophoton emissionbiostimulantlipid oxidationantioxidant capacity |
| spellingShingle | Ildikó Jócsák Henrik Gyalog Richárd Hoffmann Katalin Somfalvi-Tóth In-Vivo Biophoton Emission, Physiological and Oxidative Responses of Biostimulant-Treated Winter Wheat (<i>Triticum eastivum</i> L.) as Seed Priming Possibility, for Heat Stress Alleviation Plants wheat heat stress biophoton emission biostimulant lipid oxidation antioxidant capacity |
| title | In-Vivo Biophoton Emission, Physiological and Oxidative Responses of Biostimulant-Treated Winter Wheat (<i>Triticum eastivum</i> L.) as Seed Priming Possibility, for Heat Stress Alleviation |
| title_full | In-Vivo Biophoton Emission, Physiological and Oxidative Responses of Biostimulant-Treated Winter Wheat (<i>Triticum eastivum</i> L.) as Seed Priming Possibility, for Heat Stress Alleviation |
| title_fullStr | In-Vivo Biophoton Emission, Physiological and Oxidative Responses of Biostimulant-Treated Winter Wheat (<i>Triticum eastivum</i> L.) as Seed Priming Possibility, for Heat Stress Alleviation |
| title_full_unstemmed | In-Vivo Biophoton Emission, Physiological and Oxidative Responses of Biostimulant-Treated Winter Wheat (<i>Triticum eastivum</i> L.) as Seed Priming Possibility, for Heat Stress Alleviation |
| title_short | In-Vivo Biophoton Emission, Physiological and Oxidative Responses of Biostimulant-Treated Winter Wheat (<i>Triticum eastivum</i> L.) as Seed Priming Possibility, for Heat Stress Alleviation |
| title_sort | in vivo biophoton emission physiological and oxidative responses of biostimulant treated winter wheat i triticum eastivum i l as seed priming possibility for heat stress alleviation |
| topic | wheat heat stress biophoton emission biostimulant lipid oxidation antioxidant capacity |
| url | https://www.mdpi.com/2223-7747/11/5/640 |
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