Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration
Large-segment bone defect caused by trauma or tumor is one of the most challenging problems in orthopedic clinics. Biomimetic materials for bone tissue engineering have developed dramatically in the past few decades. The organic combination of biomimetic materials and stem cells offers new strategie...
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.770049/full |
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| author | Jun Li Wenzhao Wang Mingxin Li Ping Song Ping Song Haoyuan Lei Haoyuan Lei Xingyu Gui Xingyu Gui Changchun Zhou Changchun Zhou Lei Liu |
| author_facet | Jun Li Wenzhao Wang Mingxin Li Ping Song Ping Song Haoyuan Lei Haoyuan Lei Xingyu Gui Xingyu Gui Changchun Zhou Changchun Zhou Lei Liu |
| author_sort | Jun Li |
| collection | DOAJ |
| description | Large-segment bone defect caused by trauma or tumor is one of the most challenging problems in orthopedic clinics. Biomimetic materials for bone tissue engineering have developed dramatically in the past few decades. The organic combination of biomimetic materials and stem cells offers new strategies for tissue repair, and the fate of stem cells is closely related to their extracellular matrix (ECM) properties. In this study, a photocrosslinked biomimetic methacrylated gelatin (Bio-GelMA) hydrogel scaffold was prepared to simulate the physical structure and chemical composition of the natural bone extracellular matrix, providing a three-dimensional (3D) template and extracellular matrix microenvironment. Bone marrow mesenchymal stem cells (BMSCS) were encapsulated in Bio-GelMA scaffolds to examine the therapeutic effects of ECM-loaded cells in a 3D environment simulated for segmental bone defects. In vitro results showed that Bio-GelMA had good biocompatibility and sufficient mechanical properties (14.22kPa). A rat segmental bone defect model was constructed in vivo. The GelMA-BMSC suspension was added into the PDMS mold with the size of the bone defect and photocured as a scaffold. BMSC-loaded Bio-GelMA resulted in maximum and robust new bone formation compared with hydrogels alone and stem cell group. In conclusion, the bio-GelMA scaffold can be used as a cell carrier of BMSC to promote the repair of segmental bone defects and has great potential in future clinical applications. |
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| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-17T13:58:13Z |
| publishDate | 2021-12-01 |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-daa90d5301d041d0b7603153456a04ce2022-12-21T21:45:52ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-12-01910.3389/fbioe.2021.770049770049Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue RegenerationJun Li0Wenzhao Wang1Mingxin Li2Ping Song3Ping Song4Haoyuan Lei5Haoyuan Lei6Xingyu Gui7Xingyu Gui8Changchun Zhou9Changchun Zhou10Lei Liu11Department of Orthopedics, Orthopedic Research Institute, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Orthopedics, Orthopedic Research Institute, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Orthopedics, Orthopedic Research Institute, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, ChinaNational Engineering Research Center for Biomaterials, Sichuan University, Chengdu, ChinaCollege of Biomedical Engineering, Sichuan University, Chengdu, ChinaNational Engineering Research Center for Biomaterials, Sichuan University, Chengdu, ChinaCollege of Biomedical Engineering, Sichuan University, Chengdu, ChinaNational Engineering Research Center for Biomaterials, Sichuan University, Chengdu, ChinaCollege of Biomedical Engineering, Sichuan University, Chengdu, ChinaNational Engineering Research Center for Biomaterials, Sichuan University, Chengdu, ChinaCollege of Biomedical Engineering, Sichuan University, Chengdu, ChinaDepartment of Orthopedics, Orthopedic Research Institute, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, ChinaLarge-segment bone defect caused by trauma or tumor is one of the most challenging problems in orthopedic clinics. Biomimetic materials for bone tissue engineering have developed dramatically in the past few decades. The organic combination of biomimetic materials and stem cells offers new strategies for tissue repair, and the fate of stem cells is closely related to their extracellular matrix (ECM) properties. In this study, a photocrosslinked biomimetic methacrylated gelatin (Bio-GelMA) hydrogel scaffold was prepared to simulate the physical structure and chemical composition of the natural bone extracellular matrix, providing a three-dimensional (3D) template and extracellular matrix microenvironment. Bone marrow mesenchymal stem cells (BMSCS) were encapsulated in Bio-GelMA scaffolds to examine the therapeutic effects of ECM-loaded cells in a 3D environment simulated for segmental bone defects. In vitro results showed that Bio-GelMA had good biocompatibility and sufficient mechanical properties (14.22kPa). A rat segmental bone defect model was constructed in vivo. The GelMA-BMSC suspension was added into the PDMS mold with the size of the bone defect and photocured as a scaffold. BMSC-loaded Bio-GelMA resulted in maximum and robust new bone formation compared with hydrogels alone and stem cell group. In conclusion, the bio-GelMA scaffold can be used as a cell carrier of BMSC to promote the repair of segmental bone defects and has great potential in future clinical applications.https://www.frontiersin.org/articles/10.3389/fbioe.2021.770049/fulllarge segment bone defectGelMAhydrogelBMSCsECM |
| spellingShingle | Jun Li Wenzhao Wang Mingxin Li Ping Song Ping Song Haoyuan Lei Haoyuan Lei Xingyu Gui Xingyu Gui Changchun Zhou Changchun Zhou Lei Liu Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration Frontiers in Bioengineering and Biotechnology large segment bone defect GelMA hydrogel BMSCs ECM |
| title | Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration |
| title_full | Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration |
| title_fullStr | Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration |
| title_full_unstemmed | Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration |
| title_short | Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration |
| title_sort | biomimetic methacrylated gelatin hydrogel loaded with bone marrow mesenchymal stem cells for bone tissue regeneration |
| topic | large segment bone defect GelMA hydrogel BMSCs ECM |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.770049/full |
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