Ameliorated biomechanical properties of carotid arteries by puerarin in spontaneously hypertensive rats
Abstract Background An emerging body of evidence indicates that puerarin (PUE) plays an important role in the treatment of angina pectoris, myocardial ischemia-reperfusion injury, hypertension and other cardiovascular diseases, but how PUE affects the vascular remodeling of hypertensive rats has not...
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| Format: | Article |
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BMC
2021-06-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-021-03345-8 |
| _version_ | 1818462021184651264 |
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| author | Xiaoxia Fang Sheng Dong Yun Wu Yun He Min Lu Dandan Shi Na Feng Songhe Yin Yan Jiang Anhua Zhang Yan Ding Qiufang Zhang Junming Tang Wenjun Zhang Xiju He |
| author_facet | Xiaoxia Fang Sheng Dong Yun Wu Yun He Min Lu Dandan Shi Na Feng Songhe Yin Yan Jiang Anhua Zhang Yan Ding Qiufang Zhang Junming Tang Wenjun Zhang Xiju He |
| author_sort | Xiaoxia Fang |
| collection | DOAJ |
| description | Abstract Background An emerging body of evidence indicates that puerarin (PUE) plays an important role in the treatment of angina pectoris, myocardial ischemia-reperfusion injury, hypertension and other cardiovascular diseases, but how PUE affects the vascular remodeling of hypertensive rats has not been reported yet. This study aimed to investigate the effect and mechanism of PUE on carotid arteries of spontaneously hypertensive rats (SHR) to provide the basis for the clinical application of PUE. Methods Thirty male SHR and six male Wistar Kyoto rats (WKY) aged 3 months were used in this study, SHR rats were randomly divided into 5 groups, PUE(40 or 80 mg/kg/d, ip) and telmisartan (TELMI) (30 mg/kg/d, ig) were administrated for 3 months. We use DMT myography pressure-diameter system to investigate biomechanical properties of carotid arteries, 10 μM pan-classical transient receptor potential channels (TRPCs) inhibitor SKF96365, 200 nM specific TRPC6 inhibitor SAR7334 and 100 μM Orai1 inhibitor ANCOA4 were used in the mechanical test. Results PUE can significantly decrease systolic and diastolic blood pressure, long-term administration of PUE resulted in a mild reduction of thickness and inner diameter of carotid artery. PUE ameliorate NE-response and vascular remodeling mainly through inhibiting TRPCs channel activities of VSMC. Conclusion PUE can ameliorate biomechanical remodeling of carotid arteries through inhibiting TRPCs channel activities of VSMC in spontaneously hypertensive rats. |
| first_indexed | 2024-12-14T23:55:24Z |
| format | Article |
| id | doaj.art-daa9112c8b0c46f28714842c82566149 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-14T23:55:24Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-daa9112c8b0c46f28714842c825661492022-12-21T22:43:08ZengBMCBMC Complementary Medicine and Therapies2662-76712021-06-0121111210.1186/s12906-021-03345-8Ameliorated biomechanical properties of carotid arteries by puerarin in spontaneously hypertensive ratsXiaoxia Fang0Sheng Dong1Yun Wu2Yun He3Min Lu4Dandan Shi5Na Feng6Songhe Yin7Yan Jiang8Anhua Zhang9Yan Ding10Qiufang Zhang11Junming Tang12Wenjun Zhang13Xiju He14Department of Neurology, Taihe Hospital, Hubei University of MedicineDepartment of Anatomy, Hubei University of MedicineDepartment of Anatomy, Hubei University of MedicineDepartment of Ultrasound, Taihe Hospital, Hubei University of MedicineHubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of MedicineDepartment of Anatomy, Hubei University of MedicineDepartment of Anatomy, Hubei University of MedicineDepartment of Neurology, Taihe Hospital, Hubei University of MedicineDepartment of Ultrasound, Taihe Hospital, Hubei University of MedicineDepartment of Anatomy, Hubei University of MedicineHubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of MedicineHubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of MedicineHubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of MedicineDepartment of Ultrasound, Taihe Hospital, Hubei University of MedicineDepartment of Anatomy, Hubei University of MedicineAbstract Background An emerging body of evidence indicates that puerarin (PUE) plays an important role in the treatment of angina pectoris, myocardial ischemia-reperfusion injury, hypertension and other cardiovascular diseases, but how PUE affects the vascular remodeling of hypertensive rats has not been reported yet. This study aimed to investigate the effect and mechanism of PUE on carotid arteries of spontaneously hypertensive rats (SHR) to provide the basis for the clinical application of PUE. Methods Thirty male SHR and six male Wistar Kyoto rats (WKY) aged 3 months were used in this study, SHR rats were randomly divided into 5 groups, PUE(40 or 80 mg/kg/d, ip) and telmisartan (TELMI) (30 mg/kg/d, ig) were administrated for 3 months. We use DMT myography pressure-diameter system to investigate biomechanical properties of carotid arteries, 10 μM pan-classical transient receptor potential channels (TRPCs) inhibitor SKF96365, 200 nM specific TRPC6 inhibitor SAR7334 and 100 μM Orai1 inhibitor ANCOA4 were used in the mechanical test. Results PUE can significantly decrease systolic and diastolic blood pressure, long-term administration of PUE resulted in a mild reduction of thickness and inner diameter of carotid artery. PUE ameliorate NE-response and vascular remodeling mainly through inhibiting TRPCs channel activities of VSMC. Conclusion PUE can ameliorate biomechanical remodeling of carotid arteries through inhibiting TRPCs channel activities of VSMC in spontaneously hypertensive rats.https://doi.org/10.1186/s12906-021-03345-8HypertensionPuerarinTRPCVascular remodelingBiomechanical property |
| spellingShingle | Xiaoxia Fang Sheng Dong Yun Wu Yun He Min Lu Dandan Shi Na Feng Songhe Yin Yan Jiang Anhua Zhang Yan Ding Qiufang Zhang Junming Tang Wenjun Zhang Xiju He Ameliorated biomechanical properties of carotid arteries by puerarin in spontaneously hypertensive rats BMC Complementary Medicine and Therapies Hypertension Puerarin TRPC Vascular remodeling Biomechanical property |
| title | Ameliorated biomechanical properties of carotid arteries by puerarin in spontaneously hypertensive rats |
| title_full | Ameliorated biomechanical properties of carotid arteries by puerarin in spontaneously hypertensive rats |
| title_fullStr | Ameliorated biomechanical properties of carotid arteries by puerarin in spontaneously hypertensive rats |
| title_full_unstemmed | Ameliorated biomechanical properties of carotid arteries by puerarin in spontaneously hypertensive rats |
| title_short | Ameliorated biomechanical properties of carotid arteries by puerarin in spontaneously hypertensive rats |
| title_sort | ameliorated biomechanical properties of carotid arteries by puerarin in spontaneously hypertensive rats |
| topic | Hypertension Puerarin TRPC Vascular remodeling Biomechanical property |
| url | https://doi.org/10.1186/s12906-021-03345-8 |
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