The Secondary Structural Models (16S rRNA) of Alkaline Amylase Producing Bacillus species strain SP-RM2: Phylogenetics Analysis
Bacterial isolates especially the Bacillus strains have gained an edge over other organisms across the globe. Enzymes derived from alkaliphilic Bacillus make up about half of the total industrial enzyme market. Detergents, textiles, starch, baking and animal feed are the major industries which utmos...
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| Format: | Article |
| Language: | English |
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Bulgarian Academy of Sciences
2019-09-01
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| Series: | International Journal Bioautomation |
| Subjects: | |
| Online Access: | http://www.biomed.bas.bg/bioautomation/2019/vol_23.3/files/23.3_03.pdf |
| _version_ | 1817967850684416000 |
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| author | Sonali Priyadarshini Sukanta Kumar Pradhan Pratima Ray |
| author_facet | Sonali Priyadarshini Sukanta Kumar Pradhan Pratima Ray |
| author_sort | Sonali Priyadarshini |
| collection | DOAJ |
| description | Bacterial isolates especially the Bacillus strains have gained an edge over other organisms across the globe. Enzymes derived from alkaliphilic Bacillus make up about half of the total industrial enzyme market. Detergents, textiles, starch, baking and animal feed are the major industries which utmost produce and use enzymes. Alkaline α amylases are among the most important enzymes and are of great significance for biotechnology. In the present investigation, one alkalitolerant bacterial isolate coded RM2 was characterized by partial 16S rRNA gene sequencing due to its ability to produce alkaline amylase at pH 8.0. The amplified consensus 16S rRNA gene sequence (RM2) was further searched against nucleotide database using Blast program to find out its potential homologs. Twelve closest bacterial spp. were identified and the phylogenetic analysis revealed the target bacteria (RM2) isolate belong to genus Bacillus. RM2 has shown relatedness to Bacillus flexus strain IFO15715 in sequence based divergence study whereas secondary structure of 16S r-RNA of Bacillus sp. strain SP-RM2 did not show any relatedness to Bacillus flexus strain IFO15715 even though they shared sequence based similarity. |
| first_indexed | 2024-04-12T05:00:44Z |
| format | Article |
| id | doaj.art-daad99af55dc40f1bd7206c7d1a762ad |
| institution | Directory Open Access Journal |
| issn | 1314-1902 1314-2321 |
| language | English |
| last_indexed | 2024-04-12T05:00:44Z |
| publishDate | 2019-09-01 |
| publisher | Bulgarian Academy of Sciences |
| record_format | Article |
| series | International Journal Bioautomation |
| spelling | doaj.art-daad99af55dc40f1bd7206c7d1a762ad2022-12-22T03:47:00ZengBulgarian Academy of SciencesInternational Journal Bioautomation1314-19021314-23212019-09-0123328329210.7546/ijba.2019.23.3.000504The Secondary Structural Models (16S rRNA) of Alkaline Amylase Producing Bacillus species strain SP-RM2: Phylogenetics AnalysisSonali Priyadarshini0Sukanta Kumar PradhanPratima RayDepartment of Microbiology, College of Basic Science and Humanities, Orissa University of Agriculture and Technology, Surya Nagar, Bhubaneswar 751 003, Odisha, IndiaBacterial isolates especially the Bacillus strains have gained an edge over other organisms across the globe. Enzymes derived from alkaliphilic Bacillus make up about half of the total industrial enzyme market. Detergents, textiles, starch, baking and animal feed are the major industries which utmost produce and use enzymes. Alkaline α amylases are among the most important enzymes and are of great significance for biotechnology. In the present investigation, one alkalitolerant bacterial isolate coded RM2 was characterized by partial 16S rRNA gene sequencing due to its ability to produce alkaline amylase at pH 8.0. The amplified consensus 16S rRNA gene sequence (RM2) was further searched against nucleotide database using Blast program to find out its potential homologs. Twelve closest bacterial spp. were identified and the phylogenetic analysis revealed the target bacteria (RM2) isolate belong to genus Bacillus. RM2 has shown relatedness to Bacillus flexus strain IFO15715 in sequence based divergence study whereas secondary structure of 16S r-RNA of Bacillus sp. strain SP-RM2 did not show any relatedness to Bacillus flexus strain IFO15715 even though they shared sequence based similarity.http://www.biomed.bas.bg/bioautomation/2019/vol_23.3/files/23.3_03.pdfalkaline α amylasebacillusphylogenetic analysis |
| spellingShingle | Sonali Priyadarshini Sukanta Kumar Pradhan Pratima Ray The Secondary Structural Models (16S rRNA) of Alkaline Amylase Producing Bacillus species strain SP-RM2: Phylogenetics Analysis International Journal Bioautomation alkaline α amylase bacillus phylogenetic analysis |
| title | The Secondary Structural Models (16S rRNA) of Alkaline Amylase Producing Bacillus species strain SP-RM2: Phylogenetics Analysis |
| title_full | The Secondary Structural Models (16S rRNA) of Alkaline Amylase Producing Bacillus species strain SP-RM2: Phylogenetics Analysis |
| title_fullStr | The Secondary Structural Models (16S rRNA) of Alkaline Amylase Producing Bacillus species strain SP-RM2: Phylogenetics Analysis |
| title_full_unstemmed | The Secondary Structural Models (16S rRNA) of Alkaline Amylase Producing Bacillus species strain SP-RM2: Phylogenetics Analysis |
| title_short | The Secondary Structural Models (16S rRNA) of Alkaline Amylase Producing Bacillus species strain SP-RM2: Phylogenetics Analysis |
| title_sort | secondary structural models 16s rrna of alkaline amylase producing bacillus species strain sp rm2 phylogenetics analysis |
| topic | alkaline α amylase bacillus phylogenetic analysis |
| url | http://www.biomed.bas.bg/bioautomation/2019/vol_23.3/files/23.3_03.pdf |
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