CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE
This research aimed to characterize VirB4 protein of local isolate Brucella abortus with Western blotting method. The result showed that there were four protein bands with molecular weights of 64.61, 59.25, 21.63, and 16.70 kDa by triggering a reaction between the whole Brucella abortus and anti-Bru...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Indonesian |
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Syiah Kuala University
2018-06-01
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| Series: | Jurnal Kedokteran Hewan |
| Online Access: | https://jurnal.usk.ac.id/JKH/article/view/8091 |
| _version_ | 1797278940276981760 |
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| author | Ratih Novita Praja Didik Handijatno Setiawan Koesdarto Aditya Yudhana |
| author_facet | Ratih Novita Praja Didik Handijatno Setiawan Koesdarto Aditya Yudhana |
| author_sort | Ratih Novita Praja |
| collection | DOAJ |
| description | This research aimed to characterize VirB4 protein of local isolate Brucella abortus with Western blotting method. The result showed that there were four protein bands with molecular weights of 64.61, 59.25, 21.63, and 16.70 kDa by triggering a reaction between the whole Brucella abortus and anti-Brucella abortus serum. The results also revealed that there was only one protein band with a molecular weight of 59.25 kDa triggering a reaction between the whole Brucella abortus and anti-VirB Brucella abortus serum. Finally, it can be concluded that VirB4 protein can affect the virulence factor of Brucella abortus, successfully characterized with the appearance of one band with a molecular weight of 59.25 kDa by using Western blotting method. |
| first_indexed | 2024-03-07T16:17:19Z |
| format | Article |
| id | doaj.art-dab5324d5ac04241860ca8bcda582b8d |
| institution | Directory Open Access Journal |
| issn | 1978-225X 2502-5600 |
| language | Indonesian |
| last_indexed | 2024-03-07T16:17:19Z |
| publishDate | 2018-06-01 |
| publisher | Syiah Kuala University |
| record_format | Article |
| series | Jurnal Kedokteran Hewan |
| spelling | doaj.art-dab5324d5ac04241860ca8bcda582b8d2024-03-04T10:29:00ZindSyiah Kuala UniversityJurnal Kedokteran Hewan1978-225X2502-56002018-06-0112110.21157/j.ked.hewan.v12i1.80918334CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUERatih Novita Praja0Didik Handijatno1Setiawan Koesdarto2Aditya Yudhana31Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, IndonesiaThis research aimed to characterize VirB4 protein of local isolate Brucella abortus with Western blotting method. The result showed that there were four protein bands with molecular weights of 64.61, 59.25, 21.63, and 16.70 kDa by triggering a reaction between the whole Brucella abortus and anti-Brucella abortus serum. The results also revealed that there was only one protein band with a molecular weight of 59.25 kDa triggering a reaction between the whole Brucella abortus and anti-VirB Brucella abortus serum. Finally, it can be concluded that VirB4 protein can affect the virulence factor of Brucella abortus, successfully characterized with the appearance of one band with a molecular weight of 59.25 kDa by using Western blotting method.https://jurnal.usk.ac.id/JKH/article/view/8091 |
| spellingShingle | Ratih Novita Praja Didik Handijatno Setiawan Koesdarto Aditya Yudhana CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE Jurnal Kedokteran Hewan |
| title | CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE |
| title_full | CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE |
| title_fullStr | CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE |
| title_full_unstemmed | CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE |
| title_short | CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE |
| title_sort | characterization of virb4 protein of local isolate brucella abortus with western blotting technique |
| url | https://jurnal.usk.ac.id/JKH/article/view/8091 |
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