Faecal Microbiota Analysis of Piglets During Lactation
Antimicrobial use in animals and the potential development of antimicrobial resistance is a global concern. So, non-antimicrobial techniques for animal disease control are needed. This study aimed to determine whether neonatal ceftiofur (CF) treatment affects piglet faecal microbiomes and whether fa...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/5/762 |
| _version_ | 1797569529958629376 |
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| author | Tanya L. Nowland Valeria A. Torok Wai Y. Low Mary D. Barton Kate J. Plush Roy N. Kirkwood |
| author_facet | Tanya L. Nowland Valeria A. Torok Wai Y. Low Mary D. Barton Kate J. Plush Roy N. Kirkwood |
| author_sort | Tanya L. Nowland |
| collection | DOAJ |
| description | Antimicrobial use in animals and the potential development of antimicrobial resistance is a global concern. So, non-antimicrobial techniques for animal disease control are needed. This study aimed to determine whether neonatal ceftiofur (CF) treatment affects piglet faecal microbiomes and whether faecal microbiome transplantation (FMT) can correct it. Two focal piglets per sow were assigned to treatments as follows: cffresh (<i>n</i> = 6) received CF (3 mg/kg intramuscular) at 7 d and fresh FMT at 13 d; cffrozen (<i>n</i> = 7) received CF at 7 d and frozen FMT at 13 d; CF (<i>n</i> = 8) received CF at 7 d and no FMT; and no CF (<i>n</i> = 5) received no CF or FMT. DNA was extracted from faecal samples collected on days 7, 13, and 18 for 16S rRNA amplicon analysis. All faecal blends used for the FMT consisted of pooled donor pig faeces at 1:2 ratio with saline, delivered orally at 3 mL/kg. Alpha and beta diversity metrics increased with age (<i>p</i> < 0.05). However, no effect of antibiotic or FMT treatment was evident in 13 and 18 d old piglets (<i>p</i> > 0.05). Although no effect of treatment was observed, information regarding microbial membership during lactation was gained. |
| first_indexed | 2024-03-10T20:11:52Z |
| format | Article |
| id | doaj.art-daba163dc7ef4a7dab3c30c6fad6661a |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T20:11:52Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-daba163dc7ef4a7dab3c30c6fad6661a2023-11-19T22:52:55ZengMDPI AGAnimals2076-26152020-04-0110576210.3390/ani10050762Faecal Microbiota Analysis of Piglets During LactationTanya L. Nowland0Valeria A. Torok1Wai Y. Low2Mary D. Barton3Kate J. Plush4Roy N. Kirkwood5School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA 5400, AustraliaSouth Australian Research and Development Institute, Food Sciences, SA 5064, AustraliaThe Davies Research Centre, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, SA 5371, AustraliaSchool of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, AustraliaSunPork Group, Murarrie, QLD 4172, AustraliaSchool of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA 5400, AustraliaAntimicrobial use in animals and the potential development of antimicrobial resistance is a global concern. So, non-antimicrobial techniques for animal disease control are needed. This study aimed to determine whether neonatal ceftiofur (CF) treatment affects piglet faecal microbiomes and whether faecal microbiome transplantation (FMT) can correct it. Two focal piglets per sow were assigned to treatments as follows: cffresh (<i>n</i> = 6) received CF (3 mg/kg intramuscular) at 7 d and fresh FMT at 13 d; cffrozen (<i>n</i> = 7) received CF at 7 d and frozen FMT at 13 d; CF (<i>n</i> = 8) received CF at 7 d and no FMT; and no CF (<i>n</i> = 5) received no CF or FMT. DNA was extracted from faecal samples collected on days 7, 13, and 18 for 16S rRNA amplicon analysis. All faecal blends used for the FMT consisted of pooled donor pig faeces at 1:2 ratio with saline, delivered orally at 3 mL/kg. Alpha and beta diversity metrics increased with age (<i>p</i> < 0.05). However, no effect of antibiotic or FMT treatment was evident in 13 and 18 d old piglets (<i>p</i> > 0.05). Although no effect of treatment was observed, information regarding microbial membership during lactation was gained.https://www.mdpi.com/2076-2615/10/5/762faecal microbiome transplantationceftiofurantibioticbacteriadiversity |
| spellingShingle | Tanya L. Nowland Valeria A. Torok Wai Y. Low Mary D. Barton Kate J. Plush Roy N. Kirkwood Faecal Microbiota Analysis of Piglets During Lactation Animals faecal microbiome transplantation ceftiofur antibiotic bacteria diversity |
| title | Faecal Microbiota Analysis of Piglets During Lactation |
| title_full | Faecal Microbiota Analysis of Piglets During Lactation |
| title_fullStr | Faecal Microbiota Analysis of Piglets During Lactation |
| title_full_unstemmed | Faecal Microbiota Analysis of Piglets During Lactation |
| title_short | Faecal Microbiota Analysis of Piglets During Lactation |
| title_sort | faecal microbiota analysis of piglets during lactation |
| topic | faecal microbiome transplantation ceftiofur antibiotic bacteria diversity |
| url | https://www.mdpi.com/2076-2615/10/5/762 |
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