Mutants of Xanthomonas oryzae pv. oryzae Deficient in General Secretory Pathway Are Virulence Deficient and Unable to Secrete Xylanase
Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight, a serious disease of rice. A virulence- and xylanase-deficient mutant of Xoo was isolated following ethyl methane sulfonate (EMS) mutagenesis. A cosmid clone that restored virulence and xylanase secretion was obtained from a genomic l...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2000-04-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.4.394 |
| _version_ | 1818647046310068224 |
|---|---|
| author | Suvendra K. Ray R. Rajeshwari Ramesh V. Sonti |
| author_facet | Suvendra K. Ray R. Rajeshwari Ramesh V. Sonti |
| author_sort | Suvendra K. Ray |
| collection | DOAJ |
| description | Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight, a serious disease of rice. A virulence- and xylanase-deficient mutant of Xoo was isolated following ethyl methane sulfonate (EMS) mutagenesis. A cosmid clone that restored virulence and xylanase secretion was obtained from a genomic library by functional complementation. Trans-poson mutagenesis and marker exchange studies revealed genes on the cloned DNA that were required for xylanase production and virulence. Sequence analysis with trans-poson-specific primers revealed that these genes were homologues of xps F and xps D, which encode components of a protein secretion system in Xanthomonas campestris pv. campestris. Enzyme assays showed xylanase accumulation in the periplasmic space and cytoplasm of the xps F mutant and the complementing clone restored transport to the extracellular space. |
| first_indexed | 2024-12-17T00:56:18Z |
| format | Article |
| id | doaj.art-dabac42ae2654357aa4d075db66794d2 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T00:56:18Z |
| publishDate | 2000-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-dabac42ae2654357aa4d075db66794d22022-12-21T22:09:36ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062000-04-0113439440110.1094/MPMI.2000.13.4.394Mutants of Xanthomonas oryzae pv. oryzae Deficient in General Secretory Pathway Are Virulence Deficient and Unable to Secrete XylanaseSuvendra K. RayR. RajeshwariRamesh V. SontiXanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight, a serious disease of rice. A virulence- and xylanase-deficient mutant of Xoo was isolated following ethyl methane sulfonate (EMS) mutagenesis. A cosmid clone that restored virulence and xylanase secretion was obtained from a genomic library by functional complementation. Trans-poson mutagenesis and marker exchange studies revealed genes on the cloned DNA that were required for xylanase production and virulence. Sequence analysis with trans-poson-specific primers revealed that these genes were homologues of xps F and xps D, which encode components of a protein secretion system in Xanthomonas campestris pv. campestris. Enzyme assays showed xylanase accumulation in the periplasmic space and cytoplasm of the xps F mutant and the complementing clone restored transport to the extracellular space.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.4.394type II protein secretion |
| spellingShingle | Suvendra K. Ray R. Rajeshwari Ramesh V. Sonti Mutants of Xanthomonas oryzae pv. oryzae Deficient in General Secretory Pathway Are Virulence Deficient and Unable to Secrete Xylanase Molecular Plant-Microbe Interactions type II protein secretion |
| title | Mutants of Xanthomonas oryzae pv. oryzae Deficient in General Secretory Pathway Are Virulence Deficient and Unable to Secrete Xylanase |
| title_full | Mutants of Xanthomonas oryzae pv. oryzae Deficient in General Secretory Pathway Are Virulence Deficient and Unable to Secrete Xylanase |
| title_fullStr | Mutants of Xanthomonas oryzae pv. oryzae Deficient in General Secretory Pathway Are Virulence Deficient and Unable to Secrete Xylanase |
| title_full_unstemmed | Mutants of Xanthomonas oryzae pv. oryzae Deficient in General Secretory Pathway Are Virulence Deficient and Unable to Secrete Xylanase |
| title_short | Mutants of Xanthomonas oryzae pv. oryzae Deficient in General Secretory Pathway Are Virulence Deficient and Unable to Secrete Xylanase |
| title_sort | mutants of xanthomonas oryzae pv oryzae deficient in general secretory pathway are virulence deficient and unable to secrete xylanase |
| topic | type II protein secretion |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.4.394 |
| work_keys_str_mv | AT suvendrakray mutantsofxanthomonasoryzaepvoryzaedeficientingeneralsecretorypathwayarevirulencedeficientandunabletosecretexylanase AT rrajeshwari mutantsofxanthomonasoryzaepvoryzaedeficientingeneralsecretorypathwayarevirulencedeficientandunabletosecretexylanase AT rameshvsonti mutantsofxanthomonasoryzaepvoryzaedeficientingeneralsecretorypathwayarevirulencedeficientandunabletosecretexylanase |