A longitudinal census of the bacterial community in raw milk correlated with Staphylococcus aureus clinical mastitis infections in dairy cattle
Abstract Background Staphylococcus aureus is a common cause of clinical mastitis (CM) in dairy cattle. Optimizing the bovine mammary gland microbiota to resist S. aureus colonization is a growing area of research. However, the details of the interbacterial interactions between S. aureus and commensa...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | Animal Microbiome |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42523-022-00211-x |
| _version_ | 1797659805184163840 |
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| author | Soyoun Park Dongyun Jung Ianina Altshuler Daryna Kurban Simon Dufour Jennifer Ronholm |
| author_facet | Soyoun Park Dongyun Jung Ianina Altshuler Daryna Kurban Simon Dufour Jennifer Ronholm |
| author_sort | Soyoun Park |
| collection | DOAJ |
| description | Abstract Background Staphylococcus aureus is a common cause of clinical mastitis (CM) in dairy cattle. Optimizing the bovine mammary gland microbiota to resist S. aureus colonization is a growing area of research. However, the details of the interbacterial interactions between S. aureus and commensal bacteria, which would be required to manipulate the microbiome to resist infection, are still unknown. This study aims to characterize changes in the bovine milk bacterial community before, during, and after S. aureus CM and to compare bacterial communities present in milk between infected and healthy quarters. Methods We collected quarter-level milk samples from 698 Holstein dairy cows over an entire lactation. A total of 11 quarters from 10 cows were affected by S. aureus CM and milk samples from these 10 cows (n = 583) regardless of health status were analyzed by performing 16S rRNA gene amplicon sequencing. Results The milk microbiota of healthy quarters was distinguishable from that of S. aureus CM quarters two weeks before CM diagnosis via visual inspection. Microbial network analysis showed that 11 OTUs had negative associations with OTU0001 (Staphylococcus). A low diversity or dysbiotic milk microbiome did not necessarily correlate with increased inflammation. Specifically, Staphylococcus xylosus, Staphylococcus epidermidis, and Aerococcus urinaeequi were each abundant in milk from the quarters with low levels of inflammation. Conclusion Our results show that the udder microbiome is highly dynamic, yet a change in the abundance in certain bacteria can be a potential indicator of future S. aureus CM. This study has identified potential prophylactic bacterial species that could act as a barrier against S. aureus colonization and prevent future instances of S. aureus CM. |
| first_indexed | 2024-03-11T18:21:30Z |
| format | Article |
| id | doaj.art-dabb971d9408451fa0be5be58e40e8c5 |
| institution | Directory Open Access Journal |
| issn | 2524-4671 |
| language | English |
| last_indexed | 2024-03-11T18:21:30Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Animal Microbiome |
| spelling | doaj.art-dabb971d9408451fa0be5be58e40e8c52023-10-15T11:29:40ZengBMCAnimal Microbiome2524-46712022-11-014111310.1186/s42523-022-00211-xA longitudinal census of the bacterial community in raw milk correlated with Staphylococcus aureus clinical mastitis infections in dairy cattleSoyoun Park0Dongyun Jung1Ianina Altshuler2Daryna Kurban3Simon Dufour4Jennifer Ronholm5Faculty of Agricultural and Environmental Sciences, Macdonald Campus, McGill UniversityFaculty of Agricultural and Environmental Sciences, Macdonald Campus, McGill UniversityFaculty of Agricultural and Environmental Sciences, Macdonald Campus, McGill UniversityFaculté de Médecine Vétérinaire, Université de MontréalFaculté de Médecine Vétérinaire, Université de MontréalFaculty of Agricultural and Environmental Sciences, Macdonald Campus, McGill UniversityAbstract Background Staphylococcus aureus is a common cause of clinical mastitis (CM) in dairy cattle. Optimizing the bovine mammary gland microbiota to resist S. aureus colonization is a growing area of research. However, the details of the interbacterial interactions between S. aureus and commensal bacteria, which would be required to manipulate the microbiome to resist infection, are still unknown. This study aims to characterize changes in the bovine milk bacterial community before, during, and after S. aureus CM and to compare bacterial communities present in milk between infected and healthy quarters. Methods We collected quarter-level milk samples from 698 Holstein dairy cows over an entire lactation. A total of 11 quarters from 10 cows were affected by S. aureus CM and milk samples from these 10 cows (n = 583) regardless of health status were analyzed by performing 16S rRNA gene amplicon sequencing. Results The milk microbiota of healthy quarters was distinguishable from that of S. aureus CM quarters two weeks before CM diagnosis via visual inspection. Microbial network analysis showed that 11 OTUs had negative associations with OTU0001 (Staphylococcus). A low diversity or dysbiotic milk microbiome did not necessarily correlate with increased inflammation. Specifically, Staphylococcus xylosus, Staphylococcus epidermidis, and Aerococcus urinaeequi were each abundant in milk from the quarters with low levels of inflammation. Conclusion Our results show that the udder microbiome is highly dynamic, yet a change in the abundance in certain bacteria can be a potential indicator of future S. aureus CM. This study has identified potential prophylactic bacterial species that could act as a barrier against S. aureus colonization and prevent future instances of S. aureus CM.https://doi.org/10.1186/s42523-022-00211-xStaphylococcus aureusBovine mastitisMicrobiomeBacterial interactions |
| spellingShingle | Soyoun Park Dongyun Jung Ianina Altshuler Daryna Kurban Simon Dufour Jennifer Ronholm A longitudinal census of the bacterial community in raw milk correlated with Staphylococcus aureus clinical mastitis infections in dairy cattle Animal Microbiome Staphylococcus aureus Bovine mastitis Microbiome Bacterial interactions |
| title | A longitudinal census of the bacterial community in raw milk correlated with Staphylococcus aureus clinical mastitis infections in dairy cattle |
| title_full | A longitudinal census of the bacterial community in raw milk correlated with Staphylococcus aureus clinical mastitis infections in dairy cattle |
| title_fullStr | A longitudinal census of the bacterial community in raw milk correlated with Staphylococcus aureus clinical mastitis infections in dairy cattle |
| title_full_unstemmed | A longitudinal census of the bacterial community in raw milk correlated with Staphylococcus aureus clinical mastitis infections in dairy cattle |
| title_short | A longitudinal census of the bacterial community in raw milk correlated with Staphylococcus aureus clinical mastitis infections in dairy cattle |
| title_sort | longitudinal census of the bacterial community in raw milk correlated with staphylococcus aureus clinical mastitis infections in dairy cattle |
| topic | Staphylococcus aureus Bovine mastitis Microbiome Bacterial interactions |
| url | https://doi.org/10.1186/s42523-022-00211-x |
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