Can mobilization of bone marrow stem cells be an alternative regenerative therapy to stem cell injection in a rat model of chronic kidney disease?
Abstract Chronic kidney disease (CKD) is a priority health problem affecting 36% of Egyptians. Adipose‐derived mesenchymal stem cells (ADMSCs) have multidifferentiation capacity and the ability to restore several types of cells including damaged renal cells. Granulocyte colony‐stimulating factor (G‐...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-09-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.15448 |
| _version_ | 1797397170662408192 |
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| author | Shereen Morsy Mona F. Mansour Mohamed Abdo Yasser El‐Wazir |
| author_facet | Shereen Morsy Mona F. Mansour Mohamed Abdo Yasser El‐Wazir |
| author_sort | Shereen Morsy |
| collection | DOAJ |
| description | Abstract Chronic kidney disease (CKD) is a priority health problem affecting 36% of Egyptians. Adipose‐derived mesenchymal stem cells (ADMSCs) have multidifferentiation capacity and the ability to restore several types of cells including damaged renal cells. Granulocyte colony‐stimulating factor (G‐CSF) is known to mobilize hematopoietic stem cells from bone marrow to the peripheral circulation. The aim of this study was to compare the effect of endogenous CD34+ cells mobilization and exogenous ADMSCs administration in the treatment of a rat model of adriamycin (ADR)‐induced CKD. A total of 48 male albino rats of the local strain (200 ± 50 g) were equally divided into four groups: control negative, ADR (control positive), ADMSCs group, and G‐CSF group. Six rats from each group were sacrificed after 4 weeks and the other 6 after 12 weeks. Renal function was assessed frequently by measuring serum creatinine, albumin, urea, 24‐h urinary protein level, and hemoglobin level throughout the study. Oxidative stress markers malondialdehyde (MDA) and total antioxidant (TAO) were measured on day 28. CD‐34+ cell percentage was measured on day 9. After the sacrification of the rats, kidneys were removed for histopathological assessment. Results revealed that both ADMSCs and G‐CSF significantly improved serum creatinine, albumin, urea, 24‐h urinary protein level, and histopathological damage score, with the G‐CSF‐treated group showing better improvement in 24‐h urinary protein level, serum albumin, and histopathological damage score compared with ADMSCs‐treated group. The G‐CSF group also had significantly higher levels of CD34+ cells. Oxidative stress markers (MDA and TAO) levels were significantly improved with both therapies. We conclude that mobilization of endogenous hematopoietic stem cells by G‐CSF is more effective than exogenously injected ADMSCs in protecting the kidneys against AD‐induced toxicity. |
| first_indexed | 2024-03-09T01:06:06Z |
| format | Article |
| id | doaj.art-dabd6d0d7cb6404ea872e5ae3ffa829e |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-03-09T01:06:06Z |
| publishDate | 2022-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-dabd6d0d7cb6404ea872e5ae3ffa829e2023-12-11T09:36:42ZengWileyPhysiological Reports2051-817X2022-09-011017n/an/a10.14814/phy2.15448Can mobilization of bone marrow stem cells be an alternative regenerative therapy to stem cell injection in a rat model of chronic kidney disease?Shereen Morsy0Mona F. Mansour1Mohamed Abdo2Yasser El‐Wazir3Physiology Department, Faculty of Medicine Suez Canal University Ismailia EgyptPhysiology Department, Faculty of Medicine Suez Canal University Ismailia EgyptPhysiology Department, Faculty of Medicine Suez Canal University Ismailia EgyptPhysiology Department, Faculty of Medicine Suez Canal University Ismailia EgyptAbstract Chronic kidney disease (CKD) is a priority health problem affecting 36% of Egyptians. Adipose‐derived mesenchymal stem cells (ADMSCs) have multidifferentiation capacity and the ability to restore several types of cells including damaged renal cells. Granulocyte colony‐stimulating factor (G‐CSF) is known to mobilize hematopoietic stem cells from bone marrow to the peripheral circulation. The aim of this study was to compare the effect of endogenous CD34+ cells mobilization and exogenous ADMSCs administration in the treatment of a rat model of adriamycin (ADR)‐induced CKD. A total of 48 male albino rats of the local strain (200 ± 50 g) were equally divided into four groups: control negative, ADR (control positive), ADMSCs group, and G‐CSF group. Six rats from each group were sacrificed after 4 weeks and the other 6 after 12 weeks. Renal function was assessed frequently by measuring serum creatinine, albumin, urea, 24‐h urinary protein level, and hemoglobin level throughout the study. Oxidative stress markers malondialdehyde (MDA) and total antioxidant (TAO) were measured on day 28. CD‐34+ cell percentage was measured on day 9. After the sacrification of the rats, kidneys were removed for histopathological assessment. Results revealed that both ADMSCs and G‐CSF significantly improved serum creatinine, albumin, urea, 24‐h urinary protein level, and histopathological damage score, with the G‐CSF‐treated group showing better improvement in 24‐h urinary protein level, serum albumin, and histopathological damage score compared with ADMSCs‐treated group. The G‐CSF group also had significantly higher levels of CD34+ cells. Oxidative stress markers (MDA and TAO) levels were significantly improved with both therapies. We conclude that mobilization of endogenous hematopoietic stem cells by G‐CSF is more effective than exogenously injected ADMSCs in protecting the kidneys against AD‐induced toxicity.https://doi.org/10.14814/phy2.15448adipose tissue‐derived mesenchymal stem cellsadriamycinCD34+ stem cellschronic kidney diseasegranulocyte colony‐stimulating factormalondialdehyde |
| spellingShingle | Shereen Morsy Mona F. Mansour Mohamed Abdo Yasser El‐Wazir Can mobilization of bone marrow stem cells be an alternative regenerative therapy to stem cell injection in a rat model of chronic kidney disease? Physiological Reports adipose tissue‐derived mesenchymal stem cells adriamycin CD34+ stem cells chronic kidney disease granulocyte colony‐stimulating factor malondialdehyde |
| title | Can mobilization of bone marrow stem cells be an alternative regenerative therapy to stem cell injection in a rat model of chronic kidney disease? |
| title_full | Can mobilization of bone marrow stem cells be an alternative regenerative therapy to stem cell injection in a rat model of chronic kidney disease? |
| title_fullStr | Can mobilization of bone marrow stem cells be an alternative regenerative therapy to stem cell injection in a rat model of chronic kidney disease? |
| title_full_unstemmed | Can mobilization of bone marrow stem cells be an alternative regenerative therapy to stem cell injection in a rat model of chronic kidney disease? |
| title_short | Can mobilization of bone marrow stem cells be an alternative regenerative therapy to stem cell injection in a rat model of chronic kidney disease? |
| title_sort | can mobilization of bone marrow stem cells be an alternative regenerative therapy to stem cell injection in a rat model of chronic kidney disease |
| topic | adipose tissue‐derived mesenchymal stem cells adriamycin CD34+ stem cells chronic kidney disease granulocyte colony‐stimulating factor malondialdehyde |
| url | https://doi.org/10.14814/phy2.15448 |
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