Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars
Understanding the sequence diversity of rice blast resistance genes is important for breeding new resistant rice cultivars against the rice blast fungus Magnaporthe oryzae. In this study, we selected 24 rice cultivars with different genetic backgrounds to study the allelic diversity of rice blast re...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-07-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915612072 |
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| author | Yan WANG Jia-ming ZHAO Li-xia ZHANG Ping WANG Shi-wei WANG Hui WANG Xiao-xi WANG Zhi-heng LIU Wen-jing ZHENG |
| author_facet | Yan WANG Jia-ming ZHAO Li-xia ZHANG Ping WANG Shi-wei WANG Hui WANG Xiao-xi WANG Zhi-heng LIU Wen-jing ZHENG |
| author_sort | Yan WANG |
| collection | DOAJ |
| description | Understanding the sequence diversity of rice blast resistance genes is important for breeding new resistant rice cultivars against the rice blast fungus Magnaporthe oryzae. In this study, we selected 24 rice cultivars with different genetic backgrounds to study the allelic diversity of rice blast resistance genes Piz-t, Pita and Pik. For Piz-t, a total of 17 allelic types were found within the 24 cultivars. Blast inoculations showed that most of the mutations can affect the function of the resistance gene. For Pita, except for the difference at the 918th amino acid, a majority of the 21 mutations were detected among the cultivars. Inoculations with blast isolates carrying Avr-Pita revealed that cultivars with mutations in other sites except for the 918th amino acid did not affect the function of the Pita gene. For Pik, a total of six allelic types were found within the 24 cultivars, but five of them lost the function of the resistance gene. In addition, we found that Piz-t, Pita and Pik were expressed constitutively in the 24 rice cultivars and the expression level was not related to resistance. Our results have provided the sequence diversity information of the resistance genes Piz-t, Pita and Pik among the popular rice cultivars grown in the northeast region of China. |
| first_indexed | 2024-12-14T20:24:12Z |
| format | Article |
| id | doaj.art-dabf34b80f614055b99bacfc74fd6a9b |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-14T20:24:12Z |
| publishDate | 2016-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-dabf34b80f614055b99bacfc74fd6a9b2022-12-21T22:48:39ZengElsevierJournal of Integrative Agriculture2095-31192016-07-0115714231431Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivarsYan WANG0Jia-ming ZHAO1Li-xia ZHANG2Ping WANG3Shi-wei WANG4Hui WANG5Xiao-xi WANG6Zhi-heng LIU7Wen-jing ZHENG8Plant Protection College, Shenyang Agricultural University, Shenyang 110866, P.R. China; Agricultural Crops Molecular Improving Laboratory, Liaoning Academy of Agricultural Sciences, Shenyang 110161, P.R. ChinaAgricultural Crops Molecular Improving Laboratory, Liaoning Academy of Agricultural Sciences, Shenyang 110161, P.R. ChinaAgricultural Crops Molecular Improving Laboratory, Liaoning Academy of Agricultural Sciences, Shenyang 110161, P.R. ChinaAgricultural Crops Molecular Improving Laboratory, Liaoning Academy of Agricultural Sciences, Shenyang 110161, P.R. ChinaDepartment of Plant Pathology, China Agricultural University, Beijing 100193, P.R. ChinaAgricultural Crops Molecular Improving Laboratory, Liaoning Academy of Agricultural Sciences, Shenyang 110161, P.R. ChinaPlant Protection College, Shenyang Agricultural University, Shenyang 110866, P.R. ChinaPlant Protection College, Shenyang Agricultural University, Shenyang 110866, P.R. China; Correspondence LIU Zhi-heng, Tel: +86-24-23738857Agricultural Crops Molecular Improving Laboratory, Liaoning Academy of Agricultural Sciences, Shenyang 110161, P.R. China; ZHENG Wen-jing, Tel: +86-24-31021081Understanding the sequence diversity of rice blast resistance genes is important for breeding new resistant rice cultivars against the rice blast fungus Magnaporthe oryzae. In this study, we selected 24 rice cultivars with different genetic backgrounds to study the allelic diversity of rice blast resistance genes Piz-t, Pita and Pik. For Piz-t, a total of 17 allelic types were found within the 24 cultivars. Blast inoculations showed that most of the mutations can affect the function of the resistance gene. For Pita, except for the difference at the 918th amino acid, a majority of the 21 mutations were detected among the cultivars. Inoculations with blast isolates carrying Avr-Pita revealed that cultivars with mutations in other sites except for the 918th amino acid did not affect the function of the Pita gene. For Pik, a total of six allelic types were found within the 24 cultivars, but five of them lost the function of the resistance gene. In addition, we found that Piz-t, Pita and Pik were expressed constitutively in the 24 rice cultivars and the expression level was not related to resistance. Our results have provided the sequence diversity information of the resistance genes Piz-t, Pita and Pik among the popular rice cultivars grown in the northeast region of China.http://www.sciencedirect.com/science/article/pii/S2095311915612072resistance geneavirulence geneallelesfunctiongenetic evolution |
| spellingShingle | Yan WANG Jia-ming ZHAO Li-xia ZHANG Ping WANG Shi-wei WANG Hui WANG Xiao-xi WANG Zhi-heng LIU Wen-jing ZHENG Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars Journal of Integrative Agriculture resistance gene avirulence gene alleles function genetic evolution |
| title | Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars |
| title_full | Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars |
| title_fullStr | Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars |
| title_full_unstemmed | Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars |
| title_short | Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars |
| title_sort | analysis of the diversity and function of the alleles of the rice blast resistance genes piz t pita and pik in 24 rice cultivars |
| topic | resistance gene avirulence gene alleles function genetic evolution |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915612072 |
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