Promoter PPSP1–5-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana
Isolation of phloem-specific promoters is one of the basic conditions for improving the fiber development and resistance of ramie phloem using genetic engineering. In this study, we isolated a ramie endogenous promoter (named PPSP1-BnPSP-1) and analyzed the function of its truncated fragments in Ara...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-12-01
|
| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2020.553265/full |
| _version_ | 1818727367530512384 |
|---|---|
| author | Yunhe Wang Yaning Bao Yaning Bao Yancheng Zheng Ping’an Guo Dingxiang Peng Bo Wang |
| author_facet | Yunhe Wang Yaning Bao Yaning Bao Yancheng Zheng Ping’an Guo Dingxiang Peng Bo Wang |
| author_sort | Yunhe Wang |
| collection | DOAJ |
| description | Isolation of phloem-specific promoters is one of the basic conditions for improving the fiber development and resistance of ramie phloem using genetic engineering. In this study, we isolated a ramie endogenous promoter (named PPSP1-BnPSP-1) and analyzed the function of its truncated fragments in Arabidopsis. The results show that PPSP1-BnPSP-1 can drive the GUS reporter gene to be specifically expressed in the veins of Arabidopsis. After hormone and simulated drought treatment of the independent Arabidopsis lines carrying PPSP1-BnPSP-1 and its truncated fragments, only PPSP1–5-BnPSP-1 (−600 to −1 bp region of PPSP1-BnPSP-1) is stably expressed and exhibits phloem specificity. Our findings suggest that PPSP1–5-BnPSP-1 can be used as a phloem specific promoter for further research. |
| first_indexed | 2024-12-17T22:12:58Z |
| format | Article |
| id | doaj.art-dabf75b8cf4e4e94b359cc645cf4786e |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-17T22:12:58Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-dabf75b8cf4e4e94b359cc645cf4786e2022-12-21T21:30:42ZengFrontiers Media S.A.Frontiers in Genetics1664-80212020-12-011110.3389/fgene.2020.553265553265Promoter PPSP1–5-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thalianaYunhe Wang0Yaning Bao1Yaning Bao2Yancheng Zheng3Ping’an Guo4Dingxiang Peng5Bo Wang6MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Tobacco Science, University of Guizhou, Guiyang, ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, University of Hubei Normal, Huangshi, ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaIsolation of phloem-specific promoters is one of the basic conditions for improving the fiber development and resistance of ramie phloem using genetic engineering. In this study, we isolated a ramie endogenous promoter (named PPSP1-BnPSP-1) and analyzed the function of its truncated fragments in Arabidopsis. The results show that PPSP1-BnPSP-1 can drive the GUS reporter gene to be specifically expressed in the veins of Arabidopsis. After hormone and simulated drought treatment of the independent Arabidopsis lines carrying PPSP1-BnPSP-1 and its truncated fragments, only PPSP1–5-BnPSP-1 (−600 to −1 bp region of PPSP1-BnPSP-1) is stably expressed and exhibits phloem specificity. Our findings suggest that PPSP1–5-BnPSP-1 can be used as a phloem specific promoter for further research.https://www.frontiersin.org/articles/10.3389/fgene.2020.553265/fulltissue-specificpromoterUFW cloningGUS expressionramie [Boehmeria nivea (L.) Gaud.] |
| spellingShingle | Yunhe Wang Yaning Bao Yaning Bao Yancheng Zheng Ping’an Guo Dingxiang Peng Bo Wang Promoter PPSP1–5-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana Frontiers in Genetics tissue-specific promoter UFW cloning GUS expression ramie [Boehmeria nivea (L.) Gaud.] |
| title | Promoter PPSP1–5-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana |
| title_full | Promoter PPSP1–5-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana |
| title_fullStr | Promoter PPSP1–5-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana |
| title_full_unstemmed | Promoter PPSP1–5-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana |
| title_short | Promoter PPSP1–5-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana |
| title_sort | promoter ppsp1 5 bnpsp 1 from ramie boehmeria nivea l gaud can drive phloem specific gus expression in arabidopsis thaliana |
| topic | tissue-specific promoter UFW cloning GUS expression ramie [Boehmeria nivea (L.) Gaud.] |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2020.553265/full |
| work_keys_str_mv | AT yunhewang promoterppsp15bnpsp1fromramieboehmerianivealgaudcandrivephloemspecificgusexpressioninarabidopsisthaliana AT yaningbao promoterppsp15bnpsp1fromramieboehmerianivealgaudcandrivephloemspecificgusexpressioninarabidopsisthaliana AT yaningbao promoterppsp15bnpsp1fromramieboehmerianivealgaudcandrivephloemspecificgusexpressioninarabidopsisthaliana AT yanchengzheng promoterppsp15bnpsp1fromramieboehmerianivealgaudcandrivephloemspecificgusexpressioninarabidopsisthaliana AT pinganguo promoterppsp15bnpsp1fromramieboehmerianivealgaudcandrivephloemspecificgusexpressioninarabidopsisthaliana AT dingxiangpeng promoterppsp15bnpsp1fromramieboehmerianivealgaudcandrivephloemspecificgusexpressioninarabidopsisthaliana AT bowang promoterppsp15bnpsp1fromramieboehmerianivealgaudcandrivephloemspecificgusexpressioninarabidopsisthaliana |