Effects of Herbal Compound (IMOD) on Behavior and Expression of Alzheimer's Disease Related Genes in Streptozotocin-Rat Model of Sporadic Alzheimer’s Disease
Purpose: Sporadic Alzheimer’s disease (AD) accounts for over 95% of cases. Possible mechanisms of AD such as inflammation and oxidative stresses in the brain motivate researchers to follow many therapies which would be effective, especially in the early stages of the disease. IMOD, the herbal extrac...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2017-09-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-7-491.pdf |
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| author | Niloofar Bazazzadegan Marzieh Dehghan Shasaltaneh Kioomars Saliminejad Koorosh Kamali Mehdi Banan Hamid Reza Khorram Khorshid |
| author_facet | Niloofar Bazazzadegan Marzieh Dehghan Shasaltaneh Kioomars Saliminejad Koorosh Kamali Mehdi Banan Hamid Reza Khorram Khorshid |
| author_sort | Niloofar Bazazzadegan |
| collection | DOAJ |
| description | Purpose: Sporadic Alzheimer’s disease (AD) accounts for
over 95% of cases. Possible mechanisms of AD such as inflammation and oxidative
stresses in the brain motivate researchers to follow many therapies which would
be effective, especially in the early stages of the disease. IMOD, the herbal
extract of R. Canina, T. Vulgare and U. Dioica plant species
enriched with selenium, has anti-inflammatory, immunoregulatory and protective
effects against oxidative stress.
Methods: In this study three AD-related genes, DAXX,
NFκβ and VEGF, were chosen as candidate to investigate the
neuroprotective effect of the extract by comparing their expression levels in
the hippocampus of rat model of sporadic AD, using qPCR in the herbal-treated
and control groups. The therapeutic effects on learning and memory levels were
evaluated by Morris Water Maze (MWM) test.
Results: Gene expression results were indicative of
significant up-regulation of Vegf in
rat’s hippocampus after treatment with the herbal extract comparing to model
group (P-value= 0.001). The MWM results showed
significant changes in path length and time for finding the hidden platform in
all groups during test and the same change in the treated comparing to the
control group in memory level.
Conclusion: It could be concluded
that the herbal extract may have significant effect on gene expression but not
on behavioral level. |
| first_indexed | 2024-12-13T21:21:59Z |
| format | Article |
| id | doaj.art-dac0240b61a54b7f8110730f89fe301e |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-12-13T21:21:59Z |
| publishDate | 2017-09-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-dac0240b61a54b7f8110730f89fe301e2022-12-21T23:31:05ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082017-09-017349149410.15171/apb.2017.060APB_19604_20170728234634Effects of Herbal Compound (IMOD) on Behavior and Expression of Alzheimer's Disease Related Genes in Streptozotocin-Rat Model of Sporadic Alzheimer’s DiseaseNiloofar Bazazzadegan0Marzieh Dehghan Shasaltaneh1Kioomars Saliminejad2Koorosh Kamali3Mehdi Banan4Hamid Reza Khorram Khorshid5Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.Laboratory of Neuro-organic Chemistry, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran.Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.Purpose: Sporadic Alzheimer’s disease (AD) accounts for over 95% of cases. Possible mechanisms of AD such as inflammation and oxidative stresses in the brain motivate researchers to follow many therapies which would be effective, especially in the early stages of the disease. IMOD, the herbal extract of R. Canina, T. Vulgare and U. Dioica plant species enriched with selenium, has anti-inflammatory, immunoregulatory and protective effects against oxidative stress. Methods: In this study three AD-related genes, DAXX, NFκβ and VEGF, were chosen as candidate to investigate the neuroprotective effect of the extract by comparing their expression levels in the hippocampus of rat model of sporadic AD, using qPCR in the herbal-treated and control groups. The therapeutic effects on learning and memory levels were evaluated by Morris Water Maze (MWM) test. Results: Gene expression results were indicative of significant up-regulation of Vegf in rat’s hippocampus after treatment with the herbal extract comparing to model group (P-value= 0.001). The MWM results showed significant changes in path length and time for finding the hidden platform in all groups during test and the same change in the treated comparing to the control group in memory level. Conclusion: It could be concluded that the herbal extract may have significant effect on gene expression but not on behavioral level.http://journals.tbzmed.ac.ir/APB/Manuscript/APB-7-491.pdfAlzheimer’s diseaseGene expressionHerbal extractRat model |
| spellingShingle | Niloofar Bazazzadegan Marzieh Dehghan Shasaltaneh Kioomars Saliminejad Koorosh Kamali Mehdi Banan Hamid Reza Khorram Khorshid Effects of Herbal Compound (IMOD) on Behavior and Expression of Alzheimer's Disease Related Genes in Streptozotocin-Rat Model of Sporadic Alzheimer’s Disease Advanced Pharmaceutical Bulletin Alzheimer’s disease Gene expression Herbal extract Rat model |
| title | Effects of Herbal
Compound (IMOD) on
Behavior and Expression of Alzheimer's Disease Related Genes in
Streptozotocin-Rat Model of Sporadic Alzheimer’s Disease |
| title_full | Effects of Herbal
Compound (IMOD) on
Behavior and Expression of Alzheimer's Disease Related Genes in
Streptozotocin-Rat Model of Sporadic Alzheimer’s Disease |
| title_fullStr | Effects of Herbal
Compound (IMOD) on
Behavior and Expression of Alzheimer's Disease Related Genes in
Streptozotocin-Rat Model of Sporadic Alzheimer’s Disease |
| title_full_unstemmed | Effects of Herbal
Compound (IMOD) on
Behavior and Expression of Alzheimer's Disease Related Genes in
Streptozotocin-Rat Model of Sporadic Alzheimer’s Disease |
| title_short | Effects of Herbal
Compound (IMOD) on
Behavior and Expression of Alzheimer's Disease Related Genes in
Streptozotocin-Rat Model of Sporadic Alzheimer’s Disease |
| title_sort | effects of herbal compound imod on behavior and expression of alzheimer s disease related genes in streptozotocin rat model of sporadic alzheimer s disease |
| topic | Alzheimer’s disease Gene expression Herbal extract Rat model |
| url | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-7-491.pdf |
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