Quantitative study of biofilm yield and biomass distribution of a multi-stage moving-bed biofilm system
A multi-stage anoxic/oxic (A/O) moving-bed biofilm reactor (MBBR) system with multiple chambers was established for municipal wastewater treatment. The active biomass quantity, bioactivity, and biomass yield of a pilot-scale multi-stage MBBR were investigated in this study. The microbial activity an...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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IWA Publishing
2023-06-01
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| Series: | Journal of Water Reuse and Desalination |
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| Online Access: | http://wreuse.iwaponline.com/content/13/2/250 |
| _version_ | 1797783061119631360 |
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| author | Ning Chen Xiaodong Wang Mei Huang Zakhar Maletskyi Harsha Ratnaweera Xuejun Bi |
| author_facet | Ning Chen Xiaodong Wang Mei Huang Zakhar Maletskyi Harsha Ratnaweera Xuejun Bi |
| author_sort | Ning Chen |
| collection | DOAJ |
| description | A multi-stage anoxic/oxic (A/O) moving-bed biofilm reactor (MBBR) system with multiple chambers was established for municipal wastewater treatment. The active biomass quantity, bioactivity, and biomass yield of a pilot-scale multi-stage MBBR were investigated in this study. The microbial activity and heterotrophic yield coefficients (YH) were measured using respirometric techniques in each chamber at different temperature conditions. Meanwhile, the growth, nitrification, and denitrification rates of functional biomass were also quantified as specific respiration rate (SOUR). The total active biomass in the multi-stage A/O-MBBR system was 0.71–1.68 g COD/m2 for the aerobic reactor and 0.39–1.44 g COD/m2 for the anoxic reactor at 10–19 °C. The YH values for the anoxic reactors were 0.61–0.69, which were comparable to the recommended value of the activated sludge model (ASM1). The correlation coefficient between Nitrospira and the autotrophic specific respiration rate (SOURA) was 0.82. Meanwhile, denitrifying genera showed a significant correlation with the heterotrophic specific respiration rate (SOURH) and the active heterotrophic biomass (XH). This study provided insights into biomass distribution and the corresponding kinetic parameters for the multi-stage MBBR systems, which may serve as a reference for process design and trouble shooting.
HIGHLIGHTS
The active biomass was quantitatively investigated in a multi-stage MBBR.;
Microbial activity and heterotrophic yield coefficients were quantified in each reactor.;
The use of respirometric techniques to assess microbial performance is feasible.;
The correlation analysis of microbial community with active biomass and SOUR was carried out.;
Data and facts were provided via long-term comprehensive investigation.; |
| first_indexed | 2024-03-13T00:20:46Z |
| format | Article |
| id | doaj.art-dac47abcd45d43aeb14227cbb0d4ab7f |
| institution | Directory Open Access Journal |
| issn | 2220-1319 2408-9370 |
| language | English |
| last_indexed | 2024-03-13T00:20:46Z |
| publishDate | 2023-06-01 |
| publisher | IWA Publishing |
| record_format | Article |
| series | Journal of Water Reuse and Desalination |
| spelling | doaj.art-dac47abcd45d43aeb14227cbb0d4ab7f2023-07-11T16:27:44ZengIWA PublishingJournal of Water Reuse and Desalination2220-13192408-93702023-06-0113225026810.2166/wrd.2023.009009Quantitative study of biofilm yield and biomass distribution of a multi-stage moving-bed biofilm systemNing Chen0Xiaodong Wang1Mei Huang2Zakhar Maletskyi3Harsha Ratnaweera4Xuejun Bi5 School of Environmental and Municipal Engineering, Qingdao University of Technology, Fushun Road 11, Qingdao 266033, China School of Environmental and Municipal Engineering, Qingdao University of Technology, Fushun Road 11, Qingdao 266033, China School of Environmental and Municipal Engineering, Qingdao University of Technology, Fushun Road 11, Qingdao 266033, China Faculty of Science and Technology, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Aas, Oslo, Norway Faculty of Science and Technology, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Aas, Oslo, Norway School of Environmental and Municipal Engineering, Qingdao University of Technology, Fushun Road 11, Qingdao 266033, China A multi-stage anoxic/oxic (A/O) moving-bed biofilm reactor (MBBR) system with multiple chambers was established for municipal wastewater treatment. The active biomass quantity, bioactivity, and biomass yield of a pilot-scale multi-stage MBBR were investigated in this study. The microbial activity and heterotrophic yield coefficients (YH) were measured using respirometric techniques in each chamber at different temperature conditions. Meanwhile, the growth, nitrification, and denitrification rates of functional biomass were also quantified as specific respiration rate (SOUR). The total active biomass in the multi-stage A/O-MBBR system was 0.71–1.68 g COD/m2 for the aerobic reactor and 0.39–1.44 g COD/m2 for the anoxic reactor at 10–19 °C. The YH values for the anoxic reactors were 0.61–0.69, which were comparable to the recommended value of the activated sludge model (ASM1). The correlation coefficient between Nitrospira and the autotrophic specific respiration rate (SOURA) was 0.82. Meanwhile, denitrifying genera showed a significant correlation with the heterotrophic specific respiration rate (SOURH) and the active heterotrophic biomass (XH). This study provided insights into biomass distribution and the corresponding kinetic parameters for the multi-stage MBBR systems, which may serve as a reference for process design and trouble shooting. HIGHLIGHTS The active biomass was quantitatively investigated in a multi-stage MBBR.; Microbial activity and heterotrophic yield coefficients were quantified in each reactor.; The use of respirometric techniques to assess microbial performance is feasible.; The correlation analysis of microbial community with active biomass and SOUR was carried out.; Data and facts were provided via long-term comprehensive investigation.;http://wreuse.iwaponline.com/content/13/2/250active biomass distributionlow temperaturemoving-bed biofilm reactorrespirometric technique |
| spellingShingle | Ning Chen Xiaodong Wang Mei Huang Zakhar Maletskyi Harsha Ratnaweera Xuejun Bi Quantitative study of biofilm yield and biomass distribution of a multi-stage moving-bed biofilm system Journal of Water Reuse and Desalination active biomass distribution low temperature moving-bed biofilm reactor respirometric technique |
| title | Quantitative study of biofilm yield and biomass distribution of a multi-stage moving-bed biofilm system |
| title_full | Quantitative study of biofilm yield and biomass distribution of a multi-stage moving-bed biofilm system |
| title_fullStr | Quantitative study of biofilm yield and biomass distribution of a multi-stage moving-bed biofilm system |
| title_full_unstemmed | Quantitative study of biofilm yield and biomass distribution of a multi-stage moving-bed biofilm system |
| title_short | Quantitative study of biofilm yield and biomass distribution of a multi-stage moving-bed biofilm system |
| title_sort | quantitative study of biofilm yield and biomass distribution of a multi stage moving bed biofilm system |
| topic | active biomass distribution low temperature moving-bed biofilm reactor respirometric technique |
| url | http://wreuse.iwaponline.com/content/13/2/250 |
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