Spiking Pattern of the Mouse Developing Inner Hair Cells Is Mostly Invariant Along the Tonotopic Axis
During development, the sensory cells of the cochlea, the inner hair cells (IHCs), fire spontaneous calcium action potentials. This activity at the pre-hearing stage allows the IHCs to autonomously excite the auditory nerve fibers and hence, represents an efficient mechanism to shape the tonotopic o...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-11-01
|
| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fncel.2018.00407/full |
| _version_ | 1831811008167936000 |
|---|---|
| author | Anne-Gabrielle Harrus Jean-Charles Ceccato Gaston Sendin Jérôme Bourien Jean-Luc Puel Régis Nouvian |
| author_facet | Anne-Gabrielle Harrus Jean-Charles Ceccato Gaston Sendin Jérôme Bourien Jean-Luc Puel Régis Nouvian |
| author_sort | Anne-Gabrielle Harrus |
| collection | DOAJ |
| description | During development, the sensory cells of the cochlea, the inner hair cells (IHCs), fire spontaneous calcium action potentials. This activity at the pre-hearing stage allows the IHCs to autonomously excite the auditory nerve fibers and hence, represents an efficient mechanism to shape the tonotopic organization along the ascending auditory pathway. Using calcium imaging, we show that the activity in the developing cochlea consists of calcium waves that propagate across the supporting and sensory cells. Both basal and apical IHCs were characterized by similar spontaneous calcium transients interspaced with silent periods, consistent with bursts of action potentials recorded in patch-clamp. In addition, adjacent auditory hair cells tend to have a synchronized [Ca2+]i activity, irrespective of their location along the base-to-apex gradient of the cochlea. Finally, we show that the mechanical ablation of the inner phalangeal cells (IPCs), a class of supporting cells, reduces the synchronized [Ca2+]i activity between neighboring sensory cells. These findings support the hypothesis that the tonotopic map refinement in higher auditory centers would depend on the synchronization of a discrete number of auditory sensory cells. |
| first_indexed | 2024-12-22T21:09:01Z |
| format | Article |
| id | doaj.art-dac5475ff19b49b081a168a4584ffd38 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-22T21:09:01Z |
| publishDate | 2018-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-dac5475ff19b49b081a168a4584ffd382022-12-21T18:12:35ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022018-11-011210.3389/fncel.2018.00407405883Spiking Pattern of the Mouse Developing Inner Hair Cells Is Mostly Invariant Along the Tonotopic AxisAnne-Gabrielle HarrusJean-Charles CeccatoGaston SendinJérôme BourienJean-Luc PuelRégis NouvianDuring development, the sensory cells of the cochlea, the inner hair cells (IHCs), fire spontaneous calcium action potentials. This activity at the pre-hearing stage allows the IHCs to autonomously excite the auditory nerve fibers and hence, represents an efficient mechanism to shape the tonotopic organization along the ascending auditory pathway. Using calcium imaging, we show that the activity in the developing cochlea consists of calcium waves that propagate across the supporting and sensory cells. Both basal and apical IHCs were characterized by similar spontaneous calcium transients interspaced with silent periods, consistent with bursts of action potentials recorded in patch-clamp. In addition, adjacent auditory hair cells tend to have a synchronized [Ca2+]i activity, irrespective of their location along the base-to-apex gradient of the cochlea. Finally, we show that the mechanical ablation of the inner phalangeal cells (IPCs), a class of supporting cells, reduces the synchronized [Ca2+]i activity between neighboring sensory cells. These findings support the hypothesis that the tonotopic map refinement in higher auditory centers would depend on the synchronization of a discrete number of auditory sensory cells.https://www.frontiersin.org/article/10.3389/fncel.2018.00407/fullcochleasensory cellsaction potentialcalcium transientsspontaneous activity |
| spellingShingle | Anne-Gabrielle Harrus Jean-Charles Ceccato Gaston Sendin Jérôme Bourien Jean-Luc Puel Régis Nouvian Spiking Pattern of the Mouse Developing Inner Hair Cells Is Mostly Invariant Along the Tonotopic Axis Frontiers in Cellular Neuroscience cochlea sensory cells action potential calcium transients spontaneous activity |
| title | Spiking Pattern of the Mouse Developing Inner Hair Cells Is Mostly Invariant Along the Tonotopic Axis |
| title_full | Spiking Pattern of the Mouse Developing Inner Hair Cells Is Mostly Invariant Along the Tonotopic Axis |
| title_fullStr | Spiking Pattern of the Mouse Developing Inner Hair Cells Is Mostly Invariant Along the Tonotopic Axis |
| title_full_unstemmed | Spiking Pattern of the Mouse Developing Inner Hair Cells Is Mostly Invariant Along the Tonotopic Axis |
| title_short | Spiking Pattern of the Mouse Developing Inner Hair Cells Is Mostly Invariant Along the Tonotopic Axis |
| title_sort | spiking pattern of the mouse developing inner hair cells is mostly invariant along the tonotopic axis |
| topic | cochlea sensory cells action potential calcium transients spontaneous activity |
| url | https://www.frontiersin.org/article/10.3389/fncel.2018.00407/full |
| work_keys_str_mv | AT annegabrielleharrus spikingpatternofthemousedevelopinginnerhaircellsismostlyinvariantalongthetonotopicaxis AT jeancharlesceccato spikingpatternofthemousedevelopinginnerhaircellsismostlyinvariantalongthetonotopicaxis AT gastonsendin spikingpatternofthemousedevelopinginnerhaircellsismostlyinvariantalongthetonotopicaxis AT jeromebourien spikingpatternofthemousedevelopinginnerhaircellsismostlyinvariantalongthetonotopicaxis AT jeanlucpuel spikingpatternofthemousedevelopinginnerhaircellsismostlyinvariantalongthetonotopicaxis AT regisnouvian spikingpatternofthemousedevelopinginnerhaircellsismostlyinvariantalongthetonotopicaxis |