Features of cellular and molecular mechanisms of re-gulation of reparative processes in chronic wounds using photobiomodulation therapy
Introduction: Treatment of chronic wounds is an urgent worldwide problem for medicine. Among the many efforts in wound healing techniques, photobiomodulation therapy has shown promising results. Aim: To study the effect of photobiomodulation therapy on optimisation of the regulation...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pensoft Publishers
2022-04-01
|
| Series: | Folia Medica |
| Online Access: | https://foliamedica.bg/article/61539/download/pdf/ |
| _version_ | 1817986570115874816 |
|---|---|
| author | Sergey Pavlov Nataliia Babenko Marina Kumetchko Olga Litvinova Mykyta Valilshchykov |
| author_facet | Sergey Pavlov Nataliia Babenko Marina Kumetchko Olga Litvinova Mykyta Valilshchykov |
| author_sort | Sergey Pavlov |
| collection | DOAJ |
| description | Introduction: Treatment of chronic wounds is an urgent worldwide problem for medicine. Among the many efforts in wound healing techniques, photobiomodulation therapy has shown promising results. Aim: To study the effect of photobiomodulation therapy on optimisation of the regulation of reparative processes in chronic wounds by cytokines (through the example of interleukin-1β, tumour necrosis factor-alpha, interleukin-6, interleukin-4, interleukin-10, and granulocyte macrophage colony stimulating factor). Materials and methods: The study involved 12 Wistar rats. A trophic wound was modelled in the animals. After the operation, the animals were divided into two groups of 6 animals each. The wound defects of animals in the experimental group were exposed to low-intensity laser radiation. Photobiomodulation therapy was applied once a day for 5 days, starting the day after wound modelling. The device Lika-therapist M (Ukraine) was used in a continuous mode at a wavelength of 660 nm, an output power of 50 mW, and an energy density of 1 J/cm², and 60 s exposure time. Results: The study showed the following changes in the expression of intercellular mediators in the blood serum of animals with chronic wounds when using photobiomodulation therapy: a decrease in the levels of interleukin-1β (p>0.05) and tumour necrosis factor-alpha (p<0.05); increased concentrations of interleukin-4 (p<0.05); the concentrations of interleukin-6, granulocyte macrophage colony stimulating factor, and interleukin-10 were not significantly changed. The histological study showed better organization of collagen fibers in the experimental group. Conclusions: Photobiomodulation therapy can be an instrument for optimizing the reparative process by correcting the regulation by intercellular mediators. |
| first_indexed | 2024-04-14T00:10:55Z |
| format | Article |
| id | doaj.art-dac91cf91e3c42338258ab790cd4ce49 |
| institution | Directory Open Access Journal |
| issn | 1314-2143 |
| language | English |
| last_indexed | 2024-04-14T00:10:55Z |
| publishDate | 2022-04-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Folia Medica |
| spelling | doaj.art-dac91cf91e3c42338258ab790cd4ce492022-12-22T02:23:20ZengPensoft PublishersFolia Medica1314-21432022-04-0164226026610.3897/folmed.64.e6153961539Features of cellular and molecular mechanisms of re-gulation of reparative processes in chronic wounds using photobiomodulation therapySergey Pavlov0Nataliia Babenko1Marina Kumetchko2Olga Litvinova3Mykyta Valilshchykov4Kharkiv Medical Academy of Postgraduate EducationKharkiv Medical Academy of Postgraduate EducationKharkiv Medical Academy of Postgraduate EducationKharkiv Medical Academy of Postgraduate EducationKharkiv Medical Academy of Postgraduate EducationIntroduction: Treatment of chronic wounds is an urgent worldwide problem for medicine. Among the many efforts in wound healing techniques, photobiomodulation therapy has shown promising results. Aim: To study the effect of photobiomodulation therapy on optimisation of the regulation of reparative processes in chronic wounds by cytokines (through the example of interleukin-1β, tumour necrosis factor-alpha, interleukin-6, interleukin-4, interleukin-10, and granulocyte macrophage colony stimulating factor). Materials and methods: The study involved 12 Wistar rats. A trophic wound was modelled in the animals. After the operation, the animals were divided into two groups of 6 animals each. The wound defects of animals in the experimental group were exposed to low-intensity laser radiation. Photobiomodulation therapy was applied once a day for 5 days, starting the day after wound modelling. The device Lika-therapist M (Ukraine) was used in a continuous mode at a wavelength of 660 nm, an output power of 50 mW, and an energy density of 1 J/cm², and 60 s exposure time. Results: The study showed the following changes in the expression of intercellular mediators in the blood serum of animals with chronic wounds when using photobiomodulation therapy: a decrease in the levels of interleukin-1β (p>0.05) and tumour necrosis factor-alpha (p<0.05); increased concentrations of interleukin-4 (p<0.05); the concentrations of interleukin-6, granulocyte macrophage colony stimulating factor, and interleukin-10 were not significantly changed. The histological study showed better organization of collagen fibers in the experimental group. Conclusions: Photobiomodulation therapy can be an instrument for optimizing the reparative process by correcting the regulation by intercellular mediators.https://foliamedica.bg/article/61539/download/pdf/ |
| spellingShingle | Sergey Pavlov Nataliia Babenko Marina Kumetchko Olga Litvinova Mykyta Valilshchykov Features of cellular and molecular mechanisms of re-gulation of reparative processes in chronic wounds using photobiomodulation therapy Folia Medica |
| title | Features of cellular and molecular mechanisms of re-gulation of reparative processes in chronic wounds using photobiomodulation therapy |
| title_full | Features of cellular and molecular mechanisms of re-gulation of reparative processes in chronic wounds using photobiomodulation therapy |
| title_fullStr | Features of cellular and molecular mechanisms of re-gulation of reparative processes in chronic wounds using photobiomodulation therapy |
| title_full_unstemmed | Features of cellular and molecular mechanisms of re-gulation of reparative processes in chronic wounds using photobiomodulation therapy |
| title_short | Features of cellular and molecular mechanisms of re-gulation of reparative processes in chronic wounds using photobiomodulation therapy |
| title_sort | features of cellular and molecular mechanisms of re gulation of reparative processes in chronic wounds using photobiomodulation therapy |
| url | https://foliamedica.bg/article/61539/download/pdf/ |
| work_keys_str_mv | AT sergeypavlov featuresofcellularandmolecularmechanismsofregulationofreparativeprocessesinchronicwoundsusingphotobiomodulationtherapy AT nataliiababenko featuresofcellularandmolecularmechanismsofregulationofreparativeprocessesinchronicwoundsusingphotobiomodulationtherapy AT marinakumetchko featuresofcellularandmolecularmechanismsofregulationofreparativeprocessesinchronicwoundsusingphotobiomodulationtherapy AT olgalitvinova featuresofcellularandmolecularmechanismsofregulationofreparativeprocessesinchronicwoundsusingphotobiomodulationtherapy AT mykytavalilshchykov featuresofcellularandmolecularmechanismsofregulationofreparativeprocessesinchronicwoundsusingphotobiomodulationtherapy |