Description and Pathogenicity of Colletotrichum Species Causing Chili Anthracnose in Yogyakarta, Indonesia
Chili anthracnose caused by Colletotrichum spp. is a significant disease of chili cultivation in Indonesia. The current taxonomic status of Colletotrichum spp. has been rearranged due to a well-developed molecular study. Several species of Colletotrichum were re-identified after being analyzed by a...
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| Format: | Article |
| Language: | English |
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Universitas Brawijaya
2022-06-01
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| Series: | AGRIVITA Journal of Agricultural Science |
| Subjects: | |
| Online Access: | https://agrivita.ub.ac.id/index.php/agrivita/article/view/3705 |
| _version_ | 1811313497930203136 |
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| author | Rosa Chryse Sutomo Siti Subandiyah Arif Wibowo Ani Widiastuti |
| author_facet | Rosa Chryse Sutomo Siti Subandiyah Arif Wibowo Ani Widiastuti |
| author_sort | Rosa Chryse Sutomo |
| collection | DOAJ |
| description | Chili anthracnose caused by Colletotrichum spp. is a significant disease of chili cultivation in Indonesia. The current taxonomic status of Colletotrichum spp. has been rearranged due to a well-developed molecular study. Several species of Colletotrichum were re-identified after being analyzed by a polyphasic approach. The polyphasic method in this research combines morpho-cultural and molecular identification using ITS-rDNA and GADPH region. The pathogenicity test would be more reliable in identifying the species complexes of Colletotrichum that are difficult to differentiate. This study applies a polyphasic approach to identify Colletotrichum species causing chili anthracnose, especially in fruits collected from chili fields in Yogyakarta, Indonesia, and observe its pathogenicity in wounded and unwounded inoculation. The results of those combination methods showed that isolates collected from chili fields in Yogyakarta were C. scovillei. The pathogenicity test revealed that the fungus caused anthracnose disease in wounded and unwounded OR Twist 42 chili fruits. The unwounded infection was confirmed by amplification of the ChEC3 pathogenicity gene 24 h after inoculation, showing that appressoria production may possess an essential role in the unwounded inoculation. This study revealed that C. scovillei does not always need a wound for its pathogenicity |
| first_indexed | 2024-04-13T10:55:58Z |
| format | Article |
| id | doaj.art-dac92f8258e4434abb3ae92b0863d7ae |
| institution | Directory Open Access Journal |
| issn | 0126-0537 2477-8516 |
| language | English |
| last_indexed | 2024-04-13T10:55:58Z |
| publishDate | 2022-06-01 |
| publisher | Universitas Brawijaya |
| record_format | Article |
| series | AGRIVITA Journal of Agricultural Science |
| spelling | doaj.art-dac92f8258e4434abb3ae92b0863d7ae2022-12-22T02:49:32ZengUniversitas BrawijayaAGRIVITA Journal of Agricultural Science0126-05372477-85162022-06-0144231232110.17503/agrivita.v44i2.3705671Description and Pathogenicity of Colletotrichum Species Causing Chili Anthracnose in Yogyakarta, IndonesiaRosa Chryse Sutomo0Siti Subandiyah1Arif Wibowo2Ani Widiastuti3The Graduate School of Universitas Gadjah Mada, Yogyakarta, Indonesia 552811) The Graduate School of Universitas Gadjah Mada, Yogyakarta, Indonesia 55281 2) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281Faculty of Agriculture, Universitas Gadjah Mada1) The Graduate School of Universitas Gadjah Mada, Yogyakarta, Indonesia 55281 2) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281Chili anthracnose caused by Colletotrichum spp. is a significant disease of chili cultivation in Indonesia. The current taxonomic status of Colletotrichum spp. has been rearranged due to a well-developed molecular study. Several species of Colletotrichum were re-identified after being analyzed by a polyphasic approach. The polyphasic method in this research combines morpho-cultural and molecular identification using ITS-rDNA and GADPH region. The pathogenicity test would be more reliable in identifying the species complexes of Colletotrichum that are difficult to differentiate. This study applies a polyphasic approach to identify Colletotrichum species causing chili anthracnose, especially in fruits collected from chili fields in Yogyakarta, Indonesia, and observe its pathogenicity in wounded and unwounded inoculation. The results of those combination methods showed that isolates collected from chili fields in Yogyakarta were C. scovillei. The pathogenicity test revealed that the fungus caused anthracnose disease in wounded and unwounded OR Twist 42 chili fruits. The unwounded infection was confirmed by amplification of the ChEC3 pathogenicity gene 24 h after inoculation, showing that appressoria production may possess an essential role in the unwounded inoculation. This study revealed that C. scovillei does not always need a wound for its pathogenicityhttps://agrivita.ub.ac.id/index.php/agrivita/article/view/3705appresoriachec3gadphpolyphasic approach |
| spellingShingle | Rosa Chryse Sutomo Siti Subandiyah Arif Wibowo Ani Widiastuti Description and Pathogenicity of Colletotrichum Species Causing Chili Anthracnose in Yogyakarta, Indonesia AGRIVITA Journal of Agricultural Science appresoria chec3 gadph polyphasic approach |
| title | Description and Pathogenicity of Colletotrichum Species Causing Chili Anthracnose in Yogyakarta, Indonesia |
| title_full | Description and Pathogenicity of Colletotrichum Species Causing Chili Anthracnose in Yogyakarta, Indonesia |
| title_fullStr | Description and Pathogenicity of Colletotrichum Species Causing Chili Anthracnose in Yogyakarta, Indonesia |
| title_full_unstemmed | Description and Pathogenicity of Colletotrichum Species Causing Chili Anthracnose in Yogyakarta, Indonesia |
| title_short | Description and Pathogenicity of Colletotrichum Species Causing Chili Anthracnose in Yogyakarta, Indonesia |
| title_sort | description and pathogenicity of colletotrichum species causing chili anthracnose in yogyakarta indonesia |
| topic | appresoria chec3 gadph polyphasic approach |
| url | https://agrivita.ub.ac.id/index.php/agrivita/article/view/3705 |
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