A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEX
General cancer-targeted ligands that can deliver drugs to cells have been given considerable attention. In this paper, a high-affinity DNA aptamer (HG1) generally binding to human tumor cells was evolved by cell-SELEX, and was further optimized to have 35 deoxynucleotides (HG1-9). Aptamer HG1-9 coul...
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/16/8923 |
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| author | Nan Zhang Tao Bing Luyao Shen Le Feng Xiangjun Liu Dihua Shangguan |
| author_facet | Nan Zhang Tao Bing Luyao Shen Le Feng Xiangjun Liu Dihua Shangguan |
| author_sort | Nan Zhang |
| collection | DOAJ |
| description | General cancer-targeted ligands that can deliver drugs to cells have been given considerable attention. In this paper, a high-affinity DNA aptamer (HG1) generally binding to human tumor cells was evolved by cell-SELEX, and was further optimized to have 35 deoxynucleotides (HG1-9). Aptamer HG1-9 could be taken up by live cells, and its target protein on a cell was identified to be human transferrin receptor (TfR). As a man-made ligand of TfR, aptamer HG1-9 was demonstrated to bind at the same site of human TfR as transferrin with comparable binding affinity, and was proved to cross the epithelium barrier through transferrin receptor-mediated transcytosis. These results suggest that aptamer HG1-9 holds potential as a promising ligand to develop general cancer-targeted diagnostics and therapeutics. |
| first_indexed | 2024-03-10T08:44:20Z |
| format | Article |
| id | doaj.art-dacab00e61a2453eb66e43c4459ad367 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:44:20Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-dacab00e61a2453eb66e43c4459ad3672023-11-22T08:02:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216892310.3390/ijms22168923A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEXNan Zhang0Tao Bing1Luyao Shen2Le Feng3Xiangjun Liu4Dihua Shangguan5Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, ChinaBeijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, ChinaBeijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, ChinaBeijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, ChinaBeijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, ChinaBeijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, ChinaGeneral cancer-targeted ligands that can deliver drugs to cells have been given considerable attention. In this paper, a high-affinity DNA aptamer (HG1) generally binding to human tumor cells was evolved by cell-SELEX, and was further optimized to have 35 deoxynucleotides (HG1-9). Aptamer HG1-9 could be taken up by live cells, and its target protein on a cell was identified to be human transferrin receptor (TfR). As a man-made ligand of TfR, aptamer HG1-9 was demonstrated to bind at the same site of human TfR as transferrin with comparable binding affinity, and was proved to cross the epithelium barrier through transferrin receptor-mediated transcytosis. These results suggest that aptamer HG1-9 holds potential as a promising ligand to develop general cancer-targeted diagnostics and therapeutics.https://www.mdpi.com/1422-0067/22/16/8923aptameraptameric ligandcell-SELEXtransferrin receptortranscytosis |
| spellingShingle | Nan Zhang Tao Bing Luyao Shen Le Feng Xiangjun Liu Dihua Shangguan A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEX International Journal of Molecular Sciences aptamer aptameric ligand cell-SELEX transferrin receptor transcytosis |
| title | A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEX |
| title_full | A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEX |
| title_fullStr | A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEX |
| title_full_unstemmed | A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEX |
| title_short | A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEX |
| title_sort | dna aptameric ligand of human transferrin receptor generated by cell selex |
| topic | aptamer aptameric ligand cell-SELEX transferrin receptor transcytosis |
| url | https://www.mdpi.com/1422-0067/22/16/8923 |
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