Synergistic and Additive Interactions of Zhongshengmycin to the Chemical Insecticide Pymetrozine for Controlling Nilaparvata lugens (Hemiptera: Delphacidae)
Management of the rice brown planthopper Nilaparvata lugens Stål is challenging because it can rapidly adapt to new pesticides within several generations. Combined use of chemical insecticides and antimicrobials was proposed as an alternative strategy to control N. lugens. Our previous experiments i...
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.875610/full |
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| author | Ruoying Zhao Danting Li Xinlong Wang Zhong Li Xiaoping Yu Xuping Shentu |
| author_facet | Ruoying Zhao Danting Li Xinlong Wang Zhong Li Xiaoping Yu Xuping Shentu |
| author_sort | Ruoying Zhao |
| collection | DOAJ |
| description | Management of the rice brown planthopper Nilaparvata lugens Stål is challenging because it can rapidly adapt to new pesticides within several generations. Combined use of chemical insecticides and antimicrobials was proposed as an alternative strategy to control N. lugens. Our previous experiments identified two effective agents (chemical insecticide: pymetrozine and antimicrobial: zhongshengmycin) that act on different targets in N. lugens. However, conditions and effectiveness of combinations of antimicrobials and insecticides against N. lugens are still unknown. Here, we evaluated separate and combined effects of pymetrozine and zhongshengmycin on third instar nymphs of N. lugens under laboratory and greenhouse conditions. Results showed that zhongshengmycin exerts significant inhibitory effects on the three endosymbionts Pichia guilliermondii, Cryptococcus peneaus, and Pichia anomala cultured in vitro of N. lugens. Combinations of pymetrozine and zhongshengmycin under laboratory conditions produced additive or synergistic effects on N. lugens and caused higher mortality in third instar nymphs than either of them used alone. Experiments under greenhouse conditions further demonstrated that effective component quality ratio of pymetrozine to zhongshengmycin of 1:10 and 1:40 with co-toxicity coefficients of 221.63 and 672.87, respectively, also produced significant synergistic effects against N. lugens. Our results indicated that chemical insecticides combined with antimicrobials may provide a potential novel strategy for controlling N. lugens by inhibiting its endosymbionts. |
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| language | English |
| last_indexed | 2024-04-13T14:23:31Z |
| publishDate | 2022-05-01 |
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| series | Frontiers in Physiology |
| spelling | doaj.art-dacabb5c86914c4e82d3c06ad3a1270a2022-12-22T02:43:24ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-05-011310.3389/fphys.2022.875610875610Synergistic and Additive Interactions of Zhongshengmycin to the Chemical Insecticide Pymetrozine for Controlling Nilaparvata lugens (Hemiptera: Delphacidae)Ruoying Zhao0Danting Li1Xinlong Wang2Zhong Li3Xiaoping Yu4Xuping Shentu5Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, ChinaZhejiang Tonglu Huifeng Biosciences Co., Ltd., Hangzhou, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, ChinaManagement of the rice brown planthopper Nilaparvata lugens Stål is challenging because it can rapidly adapt to new pesticides within several generations. Combined use of chemical insecticides and antimicrobials was proposed as an alternative strategy to control N. lugens. Our previous experiments identified two effective agents (chemical insecticide: pymetrozine and antimicrobial: zhongshengmycin) that act on different targets in N. lugens. However, conditions and effectiveness of combinations of antimicrobials and insecticides against N. lugens are still unknown. Here, we evaluated separate and combined effects of pymetrozine and zhongshengmycin on third instar nymphs of N. lugens under laboratory and greenhouse conditions. Results showed that zhongshengmycin exerts significant inhibitory effects on the three endosymbionts Pichia guilliermondii, Cryptococcus peneaus, and Pichia anomala cultured in vitro of N. lugens. Combinations of pymetrozine and zhongshengmycin under laboratory conditions produced additive or synergistic effects on N. lugens and caused higher mortality in third instar nymphs than either of them used alone. Experiments under greenhouse conditions further demonstrated that effective component quality ratio of pymetrozine to zhongshengmycin of 1:10 and 1:40 with co-toxicity coefficients of 221.63 and 672.87, respectively, also produced significant synergistic effects against N. lugens. Our results indicated that chemical insecticides combined with antimicrobials may provide a potential novel strategy for controlling N. lugens by inhibiting its endosymbionts.https://www.frontiersin.org/articles/10.3389/fphys.2022.875610/fullNilaparvata lugenschemical insecticideantimicrobialscombinationsynergistic effect |
| spellingShingle | Ruoying Zhao Danting Li Xinlong Wang Zhong Li Xiaoping Yu Xuping Shentu Synergistic and Additive Interactions of Zhongshengmycin to the Chemical Insecticide Pymetrozine for Controlling Nilaparvata lugens (Hemiptera: Delphacidae) Frontiers in Physiology Nilaparvata lugens chemical insecticide antimicrobials combination synergistic effect |
| title | Synergistic and Additive Interactions of Zhongshengmycin to the Chemical Insecticide Pymetrozine for Controlling Nilaparvata lugens (Hemiptera: Delphacidae) |
| title_full | Synergistic and Additive Interactions of Zhongshengmycin to the Chemical Insecticide Pymetrozine for Controlling Nilaparvata lugens (Hemiptera: Delphacidae) |
| title_fullStr | Synergistic and Additive Interactions of Zhongshengmycin to the Chemical Insecticide Pymetrozine for Controlling Nilaparvata lugens (Hemiptera: Delphacidae) |
| title_full_unstemmed | Synergistic and Additive Interactions of Zhongshengmycin to the Chemical Insecticide Pymetrozine for Controlling Nilaparvata lugens (Hemiptera: Delphacidae) |
| title_short | Synergistic and Additive Interactions of Zhongshengmycin to the Chemical Insecticide Pymetrozine for Controlling Nilaparvata lugens (Hemiptera: Delphacidae) |
| title_sort | synergistic and additive interactions of zhongshengmycin to the chemical insecticide pymetrozine for controlling nilaparvata lugens hemiptera delphacidae |
| topic | Nilaparvata lugens chemical insecticide antimicrobials combination synergistic effect |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.875610/full |
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