MicroRNA profile of polyunsaturated fatty acid treated glioma cells reveal apoptosis-specific expression changes

<p>Abstract</p> <p>Background</p> <p>Polyunsaturated fatty acids (PUFAs) such as γ-linolenic acid (GLA), arachidonic acid (AA) and docosahexaenoic acid (DHA) have cytotoxic action on glioma cells.</p> <p>Results</p> <p>We evaluated the cytotoxic...

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Bibliographic Details
Main Authors:	Das Undurti N, Fehér Liliána Z, Kitajka Klára, Faragó Nóra, Puskás László G
Format:	Article
Language:	English
Published:	BMC 2011-09-01
Series:	Lipids in Health and Disease
Subjects:	PUFA micro RNA glioblastoma apoptosis
Online Access:	http://www.lipidworld.com/content/10/1/173

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Summary:	<p>Abstract</p> <p>Background</p> <p>Polyunsaturated fatty acids (PUFAs) such as γ-linolenic acid (GLA), arachidonic acid (AA) and docosahexaenoic acid (DHA) have cytotoxic action on glioma cells.</p> <p>Results</p> <p>We evaluated the cytotoxic action of GLA, AA and DHA on glioma cells with specific reference to the expression of miRNAs. Relative expression of miRNAs were assessed by using high throughput nanocapillary real-time PCR. Most of the miRNA target genes that showed altered expression could be classified as apoptotic genes and were up-regulated by PUFA or temozolomide treatment, while similar treatments resulted in repression of the corresponding mRNAs, such as <it>cox2</it>, <it>irs1</it>, <it>irs2</it>, <it>ccnd1</it>, <it>itgb3</it>, <it>bcl2</it>, <it>sirt1</it>, <it>tp53inp1 </it>and <it>k-ras</it>.</p> <p>Conclusions</p> <p><it>Our </it>results highlight involvement of miRNAs in the induction of apoptosis in glioma cells by fatty acids and temozolomide.</p>
ISSN:	1476-511X