Identification and Expression Analysis of an Atypical Alkaline Phosphatase in Emiliania huxleyi
Emiliania huxleyi, a cosmopolitan coccolithophore in the modern ocean, plays an important role in the carbon cycle and local climate feedback as it can form extensive blooms, calcify, and produce dimethylsulfoniopropionate (DMSP) leading to the generation of dimethyl sulfide (DMS) which affects clim...
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Frontiers Media S.A.
2018-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02156/full |
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| author | Tangcheng Li Chentao Guo Yaqun Zhang Cong Wang Xin Lin Senjie Lin Senjie Lin |
| author_facet | Tangcheng Li Chentao Guo Yaqun Zhang Cong Wang Xin Lin Senjie Lin Senjie Lin |
| author_sort | Tangcheng Li |
| collection | DOAJ |
| description | Emiliania huxleyi, a cosmopolitan coccolithophore in the modern ocean, plays an important role in the carbon cycle and local climate feedback as it can form extensive blooms, calcify, and produce dimethylsulfoniopropionate (DMSP) leading to the generation of dimethyl sulfide (DMS) which affects climate when oxidized in the atmosphere. It is known to be able to utilize dissolved organic phosphorus (DOP) by expressing a specific type of alkaline phosphatase (EHAP1) under phosphorus-limited conditions. In this study, we identified a new alkaline phosphatase (EH-PhoAaty) in this species, which we found belongs to the newly classified PhoAaty family. The expression of this atypical phosphatase was up-regulated under P-depleted conditions at both the transcriptional and translational levels, suggesting that E. huxleyi is able to express this AP to cope with phosphorus limitation. Comparative analysis revealed different transcriptional expression dynamics between eh-PhoAaty and ehap1, although both genes exhibited inducible expression under phosphate deficiency. In addition, after AP activity was eliminated by using EDTA to chelate metal ions, we found that AP activity was recovered with the supplement of Ca2+ and Zn2+, indicative of the adoption of Ca2+ as the cofactor under Zn-P co-limited conditions, likely a result of adaptation to oceanic environments where Zn2+ is often limiting. |
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| language | English |
| last_indexed | 2024-12-12T19:53:02Z |
| publishDate | 2018-09-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-dacbed4483b84b22a8cd35cabb57a3f32022-12-22T00:13:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-09-01910.3389/fmicb.2018.02156310905Identification and Expression Analysis of an Atypical Alkaline Phosphatase in Emiliania huxleyiTangcheng Li0Chentao Guo1Yaqun Zhang2Cong Wang3Xin Lin4Senjie Lin5Senjie Lin6State Key Laboratory of Marine Environmental Science and Xiamen City Key Laboratory of Urban Sea Ecological Conservation and Restoration, Xiamen University, Xiamen, ChinaState Key Laboratory of Marine Environmental Science and Xiamen City Key Laboratory of Urban Sea Ecological Conservation and Restoration, Xiamen University, Xiamen, ChinaState Key Laboratory of Marine Environmental Science and Xiamen City Key Laboratory of Urban Sea Ecological Conservation and Restoration, Xiamen University, Xiamen, ChinaState Key Laboratory of Marine Environmental Science and Xiamen City Key Laboratory of Urban Sea Ecological Conservation and Restoration, Xiamen University, Xiamen, ChinaState Key Laboratory of Marine Environmental Science and Xiamen City Key Laboratory of Urban Sea Ecological Conservation and Restoration, Xiamen University, Xiamen, ChinaState Key Laboratory of Marine Environmental Science and Xiamen City Key Laboratory of Urban Sea Ecological Conservation and Restoration, Xiamen University, Xiamen, ChinaDepartment of Marine Sciences, University of Connecticut, Groton, CT, United StatesEmiliania huxleyi, a cosmopolitan coccolithophore in the modern ocean, plays an important role in the carbon cycle and local climate feedback as it can form extensive blooms, calcify, and produce dimethylsulfoniopropionate (DMSP) leading to the generation of dimethyl sulfide (DMS) which affects climate when oxidized in the atmosphere. It is known to be able to utilize dissolved organic phosphorus (DOP) by expressing a specific type of alkaline phosphatase (EHAP1) under phosphorus-limited conditions. In this study, we identified a new alkaline phosphatase (EH-PhoAaty) in this species, which we found belongs to the newly classified PhoAaty family. The expression of this atypical phosphatase was up-regulated under P-depleted conditions at both the transcriptional and translational levels, suggesting that E. huxleyi is able to express this AP to cope with phosphorus limitation. Comparative analysis revealed different transcriptional expression dynamics between eh-PhoAaty and ehap1, although both genes exhibited inducible expression under phosphate deficiency. In addition, after AP activity was eliminated by using EDTA to chelate metal ions, we found that AP activity was recovered with the supplement of Ca2+ and Zn2+, indicative of the adoption of Ca2+ as the cofactor under Zn-P co-limited conditions, likely a result of adaptation to oceanic environments where Zn2+ is often limiting.https://www.frontiersin.org/article/10.3389/fmicb.2018.02156/fullEmiliania huxleyialkaline phosphatasegene expressionphosphorus limitationcofactor |
| spellingShingle | Tangcheng Li Chentao Guo Yaqun Zhang Cong Wang Xin Lin Senjie Lin Senjie Lin Identification and Expression Analysis of an Atypical Alkaline Phosphatase in Emiliania huxleyi Frontiers in Microbiology Emiliania huxleyi alkaline phosphatase gene expression phosphorus limitation cofactor |
| title | Identification and Expression Analysis of an Atypical Alkaline Phosphatase in Emiliania huxleyi |
| title_full | Identification and Expression Analysis of an Atypical Alkaline Phosphatase in Emiliania huxleyi |
| title_fullStr | Identification and Expression Analysis of an Atypical Alkaline Phosphatase in Emiliania huxleyi |
| title_full_unstemmed | Identification and Expression Analysis of an Atypical Alkaline Phosphatase in Emiliania huxleyi |
| title_short | Identification and Expression Analysis of an Atypical Alkaline Phosphatase in Emiliania huxleyi |
| title_sort | identification and expression analysis of an atypical alkaline phosphatase in emiliania huxleyi |
| topic | Emiliania huxleyi alkaline phosphatase gene expression phosphorus limitation cofactor |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02156/full |
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