LncRNA FOXP4-AS1 Promotes Progression of Ewing Sarcoma and Is Associated With Immune Infiltrates
Ewing sarcoma (ES) is a highly malignant primary bone tumor with poor prognosis. Studies have shown that abnormal expression of lncRNA influences the prognosis of tumor patients. Herein, we established that FOXP4-AS1 was up-regulated in ES and this correlated with poor prognosis. Further analysis il...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2021.718876/full |
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| author | Jiachao Xiong Jiachao Xiong Liang Wu Lu Huang Chunyang Wu Zhiming Liu Wenqiang Deng Shengbiao Ma Zhenhai Zhou Honggui Yu Kai Cao |
| author_facet | Jiachao Xiong Jiachao Xiong Liang Wu Lu Huang Chunyang Wu Zhiming Liu Wenqiang Deng Shengbiao Ma Zhenhai Zhou Honggui Yu Kai Cao |
| author_sort | Jiachao Xiong |
| collection | DOAJ |
| description | Ewing sarcoma (ES) is a highly malignant primary bone tumor with poor prognosis. Studies have shown that abnormal expression of lncRNA influences the prognosis of tumor patients. Herein, we established that FOXP4-AS1 was up-regulated in ES and this correlated with poor prognosis. Further analysis illustrated that FOXP4-AS1 down-regulation repression growth, migration, along with invasion of ES. On the contrary, up-regulation of FOXP4-AS1 promoted the growth, migration, as well as invasion of ES. To explore the mechanism of FOXP4-AS1, Spearman correlation analysis was carried out to determine genes that were remarkably linked to FOXP4-AS1 expression. The potential functions and pathways involving FOXP4-AS1 were identified by GO analysis, Hallmark gene set enrichment analysis, GSEA, and GSVA. The subcellular fractionation results illustrated that FOXP4-AS1 was primarily located in the cytoplasm of ES cells. Then a ceRNA network of FOXP4-AS1 was constructed. Analysis of the ceRNA network and GSEA yielded two candidate mRNAs for FOXP4-AS1. Results of the combined survival analysis led us to speculate that FOXP4-AS1 may affect the expression of TMPO by sponging miR-298, thereby regulating the malignant phenotype of ES. Finally, we found that FOXP4-AS1 may modulates the tumor immune microenvironment in an extracellular vesicle-mediated manner. In summary, FOXP4-AS1 correlates with poor prognosis of ES. It promotes the growth, migration, as well as invasion of ES cells and may modulate the tumor immune microenvironment. |
| first_indexed | 2024-12-13T16:24:14Z |
| format | Article |
| id | doaj.art-dacdcac9cc2748539b6011b727eb1150 |
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| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-13T16:24:14Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-dacdcac9cc2748539b6011b727eb11502022-12-21T23:38:38ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-10-011110.3389/fonc.2021.718876718876LncRNA FOXP4-AS1 Promotes Progression of Ewing Sarcoma and Is Associated With Immune InfiltratesJiachao Xiong0Jiachao Xiong1Liang Wu2Lu Huang3Chunyang Wu4Zhiming Liu5Wenqiang Deng6Shengbiao Ma7Zhenhai Zhou8Honggui Yu9Kai Cao10The Orthopedics Hospital, The First Affiliated Hospital of Nanchang University, Nanchang, ChinaDepartment of Orthopedics, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaThe Orthopedics Hospital, The First Affiliated Hospital of Nanchang University, Nanchang, ChinaChild Health Department of the Maternal and Children Health Hospital of Jiangxi Province, Nanchang, ChinaThe Orthopedics Hospital, The First Affiliated Hospital of Nanchang University, Nanchang, ChinaThe Orthopedics Hospital, The First Affiliated Hospital of Nanchang University, Nanchang, ChinaThe Orthopedics Hospital, The First Affiliated Hospital of Nanchang University, Nanchang, ChinaThe Orthopedics Hospital, The First Affiliated Hospital of Nanchang University, Nanchang, ChinaThe Orthopedics Hospital, The First Affiliated Hospital of Nanchang University, Nanchang, ChinaThe Orthopedics Hospital, The First Affiliated Hospital of Nanchang University, Nanchang, ChinaThe Orthopedics Hospital, The First Affiliated Hospital of Nanchang University, Nanchang, ChinaEwing sarcoma (ES) is a highly malignant primary bone tumor with poor prognosis. Studies have shown that abnormal expression of lncRNA influences the prognosis of tumor patients. Herein, we established that FOXP4-AS1 was up-regulated in ES and this correlated with poor prognosis. Further analysis illustrated that FOXP4-AS1 down-regulation repression growth, migration, along with invasion of ES. On the contrary, up-regulation of FOXP4-AS1 promoted the growth, migration, as well as invasion of ES. To explore the mechanism of FOXP4-AS1, Spearman correlation analysis was carried out to determine genes that were remarkably linked to FOXP4-AS1 expression. The potential functions and pathways involving FOXP4-AS1 were identified by GO analysis, Hallmark gene set enrichment analysis, GSEA, and GSVA. The subcellular fractionation results illustrated that FOXP4-AS1 was primarily located in the cytoplasm of ES cells. Then a ceRNA network of FOXP4-AS1 was constructed. Analysis of the ceRNA network and GSEA yielded two candidate mRNAs for FOXP4-AS1. Results of the combined survival analysis led us to speculate that FOXP4-AS1 may affect the expression of TMPO by sponging miR-298, thereby regulating the malignant phenotype of ES. Finally, we found that FOXP4-AS1 may modulates the tumor immune microenvironment in an extracellular vesicle-mediated manner. In summary, FOXP4-AS1 correlates with poor prognosis of ES. It promotes the growth, migration, as well as invasion of ES cells and may modulate the tumor immune microenvironment.https://www.frontiersin.org/articles/10.3389/fonc.2021.718876/fullFOXP4-AS1Ewing sarcomaextracellular vesiclestumor immune microenvironmentceRNA |
| spellingShingle | Jiachao Xiong Jiachao Xiong Liang Wu Lu Huang Chunyang Wu Zhiming Liu Wenqiang Deng Shengbiao Ma Zhenhai Zhou Honggui Yu Kai Cao LncRNA FOXP4-AS1 Promotes Progression of Ewing Sarcoma and Is Associated With Immune Infiltrates Frontiers in Oncology FOXP4-AS1 Ewing sarcoma extracellular vesicles tumor immune microenvironment ceRNA |
| title | LncRNA FOXP4-AS1 Promotes Progression of Ewing Sarcoma and Is Associated With Immune Infiltrates |
| title_full | LncRNA FOXP4-AS1 Promotes Progression of Ewing Sarcoma and Is Associated With Immune Infiltrates |
| title_fullStr | LncRNA FOXP4-AS1 Promotes Progression of Ewing Sarcoma and Is Associated With Immune Infiltrates |
| title_full_unstemmed | LncRNA FOXP4-AS1 Promotes Progression of Ewing Sarcoma and Is Associated With Immune Infiltrates |
| title_short | LncRNA FOXP4-AS1 Promotes Progression of Ewing Sarcoma and Is Associated With Immune Infiltrates |
| title_sort | lncrna foxp4 as1 promotes progression of ewing sarcoma and is associated with immune infiltrates |
| topic | FOXP4-AS1 Ewing sarcoma extracellular vesicles tumor immune microenvironment ceRNA |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2021.718876/full |
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