Highly Sensitive Uric Acid Detection Based on a Graphene Chemoresistor and Magnetic Beads
In this study, we developed a low-cost, reusable, and highly sensitive analytical platform for the detection of the human metabolite uric acid (UA). This novel analysis platform combines the graphene chemoresistor detection technique with a magnetic bead (MB) system. The heterojunction (single-layer...
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MDPI AG
2021-08-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/9/304 |
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| author | Wangyang Zhang Xiaoqiang Zhao Lina Diao Hao Li Zhonghao Tong Zhiqi Gu Bin Miao Zhan Xu Han Zhang Yue Wu Jiadong Li |
| author_facet | Wangyang Zhang Xiaoqiang Zhao Lina Diao Hao Li Zhonghao Tong Zhiqi Gu Bin Miao Zhan Xu Han Zhang Yue Wu Jiadong Li |
| author_sort | Wangyang Zhang |
| collection | DOAJ |
| description | In this study, we developed a low-cost, reusable, and highly sensitive analytical platform for the detection of the human metabolite uric acid (UA). This novel analysis platform combines the graphene chemoresistor detection technique with a magnetic bead (MB) system. The heterojunction (single-layer graphene and HfO<sub>2</sub> thin-film material) of our graphene-based biosensor worked as a transducer to detect the pH change caused by the specific catalytic reaction between UA and uricase, and hence acquires a UA concentration. Immobilization of uricase on MBs can decouple the functionalization steps from the sensor surface, which allows the sensor to be reusable. Our microsensor platform exhibits a relatively lower detection limit (1 μM), high sensitivity (5.6 mV/decade), a linear range (from 1 μM to 1000 μM), and excellent linearity (R<sup>2</sup> = 0.9945). In addition, interference assay and repeatability tests were conducted, and the result suggests that our method is highly stable and not affected by common interfering substances (glucose and urea). The integration of this high-performance and compact biosensor device can create a point-of-care diagnosis system with reduced cost, test time, and reagent volume. |
| first_indexed | 2024-03-10T07:51:43Z |
| format | Article |
| id | doaj.art-dacf71b500364dbfac629c514dc50d0d |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T07:51:43Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-dacf71b500364dbfac629c514dc50d0d2023-11-22T12:12:20ZengMDPI AGBiosensors2079-63742021-08-0111930410.3390/bios11090304Highly Sensitive Uric Acid Detection Based on a Graphene Chemoresistor and Magnetic BeadsWangyang Zhang0Xiaoqiang Zhao1Lina Diao2Hao Li3Zhonghao Tong4Zhiqi Gu5Bin Miao6Zhan Xu7Han Zhang8Yue Wu9Jiadong Li10Key Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, ChinaCollege of Mechatronic Engineering, North University of China, Taiyuan 030051, ChinaKey Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, ChinaKey Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, ChinaKey Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, ChinaKey Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, ChinaKey Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, ChinaKey Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, ChinaElectrical and Computer Engineering, Texas A&M University, College Station, TX 77843, USACollege of Mechatronic Engineering, North University of China, Taiyuan 030051, ChinaKey Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, ChinaIn this study, we developed a low-cost, reusable, and highly sensitive analytical platform for the detection of the human metabolite uric acid (UA). This novel analysis platform combines the graphene chemoresistor detection technique with a magnetic bead (MB) system. The heterojunction (single-layer graphene and HfO<sub>2</sub> thin-film material) of our graphene-based biosensor worked as a transducer to detect the pH change caused by the specific catalytic reaction between UA and uricase, and hence acquires a UA concentration. Immobilization of uricase on MBs can decouple the functionalization steps from the sensor surface, which allows the sensor to be reusable. Our microsensor platform exhibits a relatively lower detection limit (1 μM), high sensitivity (5.6 mV/decade), a linear range (from 1 μM to 1000 μM), and excellent linearity (R<sup>2</sup> = 0.9945). In addition, interference assay and repeatability tests were conducted, and the result suggests that our method is highly stable and not affected by common interfering substances (glucose and urea). The integration of this high-performance and compact biosensor device can create a point-of-care diagnosis system with reduced cost, test time, and reagent volume.https://www.mdpi.com/2079-6374/11/9/304graphenepH detectionuric acid detectionmagnetic beadschemoresistor |
| spellingShingle | Wangyang Zhang Xiaoqiang Zhao Lina Diao Hao Li Zhonghao Tong Zhiqi Gu Bin Miao Zhan Xu Han Zhang Yue Wu Jiadong Li Highly Sensitive Uric Acid Detection Based on a Graphene Chemoresistor and Magnetic Beads Biosensors graphene pH detection uric acid detection magnetic beads chemoresistor |
| title | Highly Sensitive Uric Acid Detection Based on a Graphene Chemoresistor and Magnetic Beads |
| title_full | Highly Sensitive Uric Acid Detection Based on a Graphene Chemoresistor and Magnetic Beads |
| title_fullStr | Highly Sensitive Uric Acid Detection Based on a Graphene Chemoresistor and Magnetic Beads |
| title_full_unstemmed | Highly Sensitive Uric Acid Detection Based on a Graphene Chemoresistor and Magnetic Beads |
| title_short | Highly Sensitive Uric Acid Detection Based on a Graphene Chemoresistor and Magnetic Beads |
| title_sort | highly sensitive uric acid detection based on a graphene chemoresistor and magnetic beads |
| topic | graphene pH detection uric acid detection magnetic beads chemoresistor |
| url | https://www.mdpi.com/2079-6374/11/9/304 |
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