Comparison of effects of aminosalicylic acid, glucocorticoids and immunosuppressive agents on the expression of multidrug-resistant genes in ulcerative colitis
Abstract To compare the effects of aminosalicylic acid, glucocorticoids and immunosuppressants on the expression levels of multidrug resistance genes in patients with ulcerative colitis (UC), with the aim of providing a theoretical and therapeutic basis for the diagnosis, treatment, and prevention o...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-19612-8 |
| _version_ | 1811190434560475136 |
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| author | Yan Chen Ping Wang Yin Zhang Xiao-Yu Du Ying-Jian Zhang |
| author_facet | Yan Chen Ping Wang Yin Zhang Xiao-Yu Du Ying-Jian Zhang |
| author_sort | Yan Chen |
| collection | DOAJ |
| description | Abstract To compare the effects of aminosalicylic acid, glucocorticoids and immunosuppressants on the expression levels of multidrug resistance genes in patients with ulcerative colitis (UC), with the aim of providing a theoretical and therapeutic basis for the diagnosis, treatment, and prevention of UC. Fresh colonic mucosal tissues or postoperative pathological biopsies from 148 UC patients were collected, and the distribution sites and morphology of P-glycoprotein (P-gp) were detected using immunohistochemical staining. RT-PCR was used to quantify the expression levels of multidrug resistance gene (MDR1) mRNA before and after the corresponding treatment, and the effects of aminosalicylic acid, glucocorticoids and immunosuppressive drugs on P-gp were compared. In addition, the effects of the three drugs on MDR1 mRNA were analyzed. Administration of 5-aminosalicylic acid (5-ASA) drugs did not correlate with MDR1 expression in UC, whereas administration of glucocorticoids and immunosuppressive drugs was positively correlated with MDR1 expression profile. The expression levels of MDR1 mRNA and its product P-gp were significantly upregulated in patients who did not respond to glucocorticoids and immunosuppressive drugs. 5-ASA had no effect on the expression levels of MDR1 and its product P-gp in patients with a confirmed diagnosis of UC. However, the use of glucocorticoids and immunosuppressants can increase the expression level of MDR1. |
| first_indexed | 2024-04-11T14:51:07Z |
| format | Article |
| id | doaj.art-dad27cb803c14285bfb4c26f630b054a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T14:51:07Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-dad27cb803c14285bfb4c26f630b054a2022-12-22T04:17:28ZengNature PortfolioScientific Reports2045-23222022-11-0112111210.1038/s41598-022-19612-8Comparison of effects of aminosalicylic acid, glucocorticoids and immunosuppressive agents on the expression of multidrug-resistant genes in ulcerative colitisYan Chen0Ping Wang1Yin Zhang2Xiao-Yu Du3Ying-Jian Zhang4Department of Gastroenterology, The First Affiliated Hospital, College of Clinical Medicine of Henan University of Science and TechnologyDepartment of Public Health, School of Medicine, Henan University of Science and TechnologyDepartment of Gastroenterology, The First Affiliated Hospital, College of Clinical Medicine of Henan University of Science and TechnologyDepartment of Gastroenterology, The First Affiliated Hospital, College of Clinical Medicine of Henan University of Science and TechnologyDepartment of Gastroenterology, The First Affiliated Hospital, College of Clinical Medicine of Henan University of Science and TechnologyAbstract To compare the effects of aminosalicylic acid, glucocorticoids and immunosuppressants on the expression levels of multidrug resistance genes in patients with ulcerative colitis (UC), with the aim of providing a theoretical and therapeutic basis for the diagnosis, treatment, and prevention of UC. Fresh colonic mucosal tissues or postoperative pathological biopsies from 148 UC patients were collected, and the distribution sites and morphology of P-glycoprotein (P-gp) were detected using immunohistochemical staining. RT-PCR was used to quantify the expression levels of multidrug resistance gene (MDR1) mRNA before and after the corresponding treatment, and the effects of aminosalicylic acid, glucocorticoids and immunosuppressive drugs on P-gp were compared. In addition, the effects of the three drugs on MDR1 mRNA were analyzed. Administration of 5-aminosalicylic acid (5-ASA) drugs did not correlate with MDR1 expression in UC, whereas administration of glucocorticoids and immunosuppressive drugs was positively correlated with MDR1 expression profile. The expression levels of MDR1 mRNA and its product P-gp were significantly upregulated in patients who did not respond to glucocorticoids and immunosuppressive drugs. 5-ASA had no effect on the expression levels of MDR1 and its product P-gp in patients with a confirmed diagnosis of UC. However, the use of glucocorticoids and immunosuppressants can increase the expression level of MDR1.https://doi.org/10.1038/s41598-022-19612-8 |
| spellingShingle | Yan Chen Ping Wang Yin Zhang Xiao-Yu Du Ying-Jian Zhang Comparison of effects of aminosalicylic acid, glucocorticoids and immunosuppressive agents on the expression of multidrug-resistant genes in ulcerative colitis Scientific Reports |
| title | Comparison of effects of aminosalicylic acid, glucocorticoids and immunosuppressive agents on the expression of multidrug-resistant genes in ulcerative colitis |
| title_full | Comparison of effects of aminosalicylic acid, glucocorticoids and immunosuppressive agents on the expression of multidrug-resistant genes in ulcerative colitis |
| title_fullStr | Comparison of effects of aminosalicylic acid, glucocorticoids and immunosuppressive agents on the expression of multidrug-resistant genes in ulcerative colitis |
| title_full_unstemmed | Comparison of effects of aminosalicylic acid, glucocorticoids and immunosuppressive agents on the expression of multidrug-resistant genes in ulcerative colitis |
| title_short | Comparison of effects of aminosalicylic acid, glucocorticoids and immunosuppressive agents on the expression of multidrug-resistant genes in ulcerative colitis |
| title_sort | comparison of effects of aminosalicylic acid glucocorticoids and immunosuppressive agents on the expression of multidrug resistant genes in ulcerative colitis |
| url | https://doi.org/10.1038/s41598-022-19612-8 |
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