Insertion site preference of Mu, Tn5, and Tn7 transposons
<p>Abstract</p> <p>Background</p> <p>Transposons, segments of DNA that can mobilize to other locations in a genome, are often used for insertion mutagenesis or to generate priming sites for sequencing of large DNA molecules. For both of these uses, a transposon with min...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-02-01
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| Series: | Mobile DNA |
| Subjects: | |
| Online Access: | http://www.mobilednajournal.com/content/3/1/3 |
| _version_ | 1818177825650245632 |
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| author | Green Brian Bouchier Christiane Fairhead Cécile Craig Nancy L Cormack Brendan P |
| author_facet | Green Brian Bouchier Christiane Fairhead Cécile Craig Nancy L Cormack Brendan P |
| author_sort | Green Brian |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Transposons, segments of DNA that can mobilize to other locations in a genome, are often used for insertion mutagenesis or to generate priming sites for sequencing of large DNA molecules. For both of these uses, a transposon with minimal insertion bias is desired to allow complete coverage with minimal oversampling.</p> <p>Findings</p> <p>Three transposons, Mu, Tn5, and Tn7, were used to generate insertions in the same set of fosmids containing <it>Candida glabrata </it>genomic DNA. Tn7 demonstrates markedly less insertion bias than either Mu or Tn5, with both Mu and Tn5 biased toward sequences containing guanosine (G) and cytidine (C). This preference of Mu and Tn5 yields less uniform spacing of insertions than for Tn7, in the adenosine (A) and thymidine (T) rich genome of <it>C. glabrata </it>(39% GC).</p> <p>Conclusions</p> <p>In light of its more uniform distribution of insertions, Tn7 should be considered for applications in which insertion bias is deleterious.</p> |
| first_indexed | 2024-12-11T20:38:14Z |
| format | Article |
| id | doaj.art-dad4530c81ca44348b125a5deaeb7b4b |
| institution | Directory Open Access Journal |
| issn | 1759-8753 |
| language | English |
| last_indexed | 2024-12-11T20:38:14Z |
| publishDate | 2012-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Mobile DNA |
| spelling | doaj.art-dad4530c81ca44348b125a5deaeb7b4b2022-12-22T00:51:35ZengBMCMobile DNA1759-87532012-02-0131310.1186/1759-8753-3-3Insertion site preference of Mu, Tn5, and Tn7 transposonsGreen BrianBouchier ChristianeFairhead CécileCraig Nancy LCormack Brendan P<p>Abstract</p> <p>Background</p> <p>Transposons, segments of DNA that can mobilize to other locations in a genome, are often used for insertion mutagenesis or to generate priming sites for sequencing of large DNA molecules. For both of these uses, a transposon with minimal insertion bias is desired to allow complete coverage with minimal oversampling.</p> <p>Findings</p> <p>Three transposons, Mu, Tn5, and Tn7, were used to generate insertions in the same set of fosmids containing <it>Candida glabrata </it>genomic DNA. Tn7 demonstrates markedly less insertion bias than either Mu or Tn5, with both Mu and Tn5 biased toward sequences containing guanosine (G) and cytidine (C). This preference of Mu and Tn5 yields less uniform spacing of insertions than for Tn7, in the adenosine (A) and thymidine (T) rich genome of <it>C. glabrata </it>(39% GC).</p> <p>Conclusions</p> <p>In light of its more uniform distribution of insertions, Tn7 should be considered for applications in which insertion bias is deleterious.</p>http://www.mobilednajournal.com/content/3/1/3Tn7MuTn5MutagenesisInsertion siteDNA transposonMobile element |
| spellingShingle | Green Brian Bouchier Christiane Fairhead Cécile Craig Nancy L Cormack Brendan P Insertion site preference of Mu, Tn5, and Tn7 transposons Mobile DNA Tn7 Mu Tn5 Mutagenesis Insertion site DNA transposon Mobile element |
| title | Insertion site preference of Mu, Tn5, and Tn7 transposons |
| title_full | Insertion site preference of Mu, Tn5, and Tn7 transposons |
| title_fullStr | Insertion site preference of Mu, Tn5, and Tn7 transposons |
| title_full_unstemmed | Insertion site preference of Mu, Tn5, and Tn7 transposons |
| title_short | Insertion site preference of Mu, Tn5, and Tn7 transposons |
| title_sort | insertion site preference of mu tn5 and tn7 transposons |
| topic | Tn7 Mu Tn5 Mutagenesis Insertion site DNA transposon Mobile element |
| url | http://www.mobilednajournal.com/content/3/1/3 |
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