In vitro evaluation of the cytotoxic and genotoxic effects of Al and Mn in ambient concentrations detected in groundwater intended for human consumption
Environmental exposure to metals can induce cytotoxic and genotoxic effects in cells and affect the health of the exposed population. To investigate the effects of aluminum (Al) and manganese (Mn), we evaluated their cytogenotoxicity using peripheral blood mononuclear cells (PBMCs) exposed to these...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323009193 |
| _version_ | 1797676716159664128 |
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| author | Luiza Flavia Veiga Francisco Debora da Silva Baldivia Bruno do Amaral Crispim Adrivanio Baranoski Syla Maria Farias Ferraz Klafke Edson Lucas dos Santos Rodrigo Juliano Oliveira Alexeia Barufatti |
| author_facet | Luiza Flavia Veiga Francisco Debora da Silva Baldivia Bruno do Amaral Crispim Adrivanio Baranoski Syla Maria Farias Ferraz Klafke Edson Lucas dos Santos Rodrigo Juliano Oliveira Alexeia Barufatti |
| author_sort | Luiza Flavia Veiga Francisco |
| collection | DOAJ |
| description | Environmental exposure to metals can induce cytotoxic and genotoxic effects in cells and affect the health of the exposed population. To investigate the effects of aluminum (Al) and manganese (Mn), we evaluated their cytogenotoxicity using peripheral blood mononuclear cells (PBMCs) exposed to these metals at previously quantified concentrations in groundwater intended for human consumption. The cell viability, membrane integrity, nuclear division index (NDI), oxidative stress, cell death, cell cycle, and DNA damage were analyzed in PBMCs exposed to Al (0.2, 0.6, and 0.8 mg/L) and Mn (0.1, 0.3, 1.0, and 1.5 for 48 h. We found that Al induced late apoptosis; decreased cell viability, NDI, membrane integrity; and increased DNA damage. However, no significant alterations in the early apoptosis, cell cycle, and reactive oxygen species levels were observed. In contrast, exposure to Mn altered all evaluated parameters related to cytogenotoxicity. Our data show that even concentrations allowed by the Brazilian legislation for Al and Mn in groundwater intended for human consumption cause cytotoxic and genotoxic effects in PBMCs. Therefore, in view of the results found, a comprehensive approach through in vivo investigations is needed to give robustness and validity to the results obtained, thus broadening the understanding of the impacts of metals on the health of environmentally exposed people. |
| first_indexed | 2024-03-11T22:32:19Z |
| format | Article |
| id | doaj.art-dad89385e3514319a41c3ac9e996c71b |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-11T22:32:19Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-dad89385e3514319a41c3ac9e996c71b2023-09-23T05:09:56ZengElsevierEcotoxicology and Environmental Safety0147-65132023-10-01264115415In vitro evaluation of the cytotoxic and genotoxic effects of Al and Mn in ambient concentrations detected in groundwater intended for human consumptionLuiza Flavia Veiga Francisco0Debora da Silva Baldivia1Bruno do Amaral Crispim2Adrivanio Baranoski3Syla Maria Farias Ferraz Klafke4Edson Lucas dos Santos5Rodrigo Juliano Oliveira6Alexeia Barufatti7Postgraduate Program in Environmental Science and Technology, Faculty of Exact Sciences and Technology, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul 79804-970, Brazil; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, São Paulo 14784-400, BrazilResearch Group on Biotechnology and Bioprospecting Applied to Metabolism, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul 79804-970, BrazilPostgraduate Program in Biodiversity and Environment, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul 79804-970, BrazilStem Cell, Cell Therapy and Toxicological Genetics Research Centre, Medical School, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul 79080-190, BrazilFaculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul 79804-970, BrazilResearch Group on Biotechnology and Bioprospecting Applied to Metabolism, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul 79804-970, BrazilStem Cell, Cell Therapy and Toxicological Genetics Research Centre, Medical School, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul 79080-190, BrazilPostgraduate Program in Environmental Science and Technology, Faculty of Exact Sciences and Technology, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul 79804-970, Brazil; Postgraduate Program in Biodiversity and Environment, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul 79804-970, Brazil; Corresponding author at: Postgraduate Program in Environmental Science and Technology, Faculty of Exact Sciences and Technology, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul 79804-970, Brazil.Environmental exposure to metals can induce cytotoxic and genotoxic effects in cells and affect the health of the exposed population. To investigate the effects of aluminum (Al) and manganese (Mn), we evaluated their cytogenotoxicity using peripheral blood mononuclear cells (PBMCs) exposed to these metals at previously quantified concentrations in groundwater intended for human consumption. The cell viability, membrane integrity, nuclear division index (NDI), oxidative stress, cell death, cell cycle, and DNA damage were analyzed in PBMCs exposed to Al (0.2, 0.6, and 0.8 mg/L) and Mn (0.1, 0.3, 1.0, and 1.5 for 48 h. We found that Al induced late apoptosis; decreased cell viability, NDI, membrane integrity; and increased DNA damage. However, no significant alterations in the early apoptosis, cell cycle, and reactive oxygen species levels were observed. In contrast, exposure to Mn altered all evaluated parameters related to cytogenotoxicity. Our data show that even concentrations allowed by the Brazilian legislation for Al and Mn in groundwater intended for human consumption cause cytotoxic and genotoxic effects in PBMCs. Therefore, in view of the results found, a comprehensive approach through in vivo investigations is needed to give robustness and validity to the results obtained, thus broadening the understanding of the impacts of metals on the health of environmentally exposed people.http://www.sciencedirect.com/science/article/pii/S0147651323009193CytotoxicityGenotoxicityPeripheral blood mononuclear cellsEnvironmental exposureMetals |
| spellingShingle | Luiza Flavia Veiga Francisco Debora da Silva Baldivia Bruno do Amaral Crispim Adrivanio Baranoski Syla Maria Farias Ferraz Klafke Edson Lucas dos Santos Rodrigo Juliano Oliveira Alexeia Barufatti In vitro evaluation of the cytotoxic and genotoxic effects of Al and Mn in ambient concentrations detected in groundwater intended for human consumption Ecotoxicology and Environmental Safety Cytotoxicity Genotoxicity Peripheral blood mononuclear cells Environmental exposure Metals |
| title | In vitro evaluation of the cytotoxic and genotoxic effects of Al and Mn in ambient concentrations detected in groundwater intended for human consumption |
| title_full | In vitro evaluation of the cytotoxic and genotoxic effects of Al and Mn in ambient concentrations detected in groundwater intended for human consumption |
| title_fullStr | In vitro evaluation of the cytotoxic and genotoxic effects of Al and Mn in ambient concentrations detected in groundwater intended for human consumption |
| title_full_unstemmed | In vitro evaluation of the cytotoxic and genotoxic effects of Al and Mn in ambient concentrations detected in groundwater intended for human consumption |
| title_short | In vitro evaluation of the cytotoxic and genotoxic effects of Al and Mn in ambient concentrations detected in groundwater intended for human consumption |
| title_sort | in vitro evaluation of the cytotoxic and genotoxic effects of al and mn in ambient concentrations detected in groundwater intended for human consumption |
| topic | Cytotoxicity Genotoxicity Peripheral blood mononuclear cells Environmental exposure Metals |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323009193 |
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