Influence of Salinity on Growth and Biochemical Composition of Green Alga Botryococcus braunii Kütz IPPAS H-252
The effect of 0,3 and 0,7 M NaCl on biomass yield, total nitrogen content, intracellular lipid content, hydrocarbons and fatty acid profile of the lipids of the alga Botryococcus braunii Kütz IPPAS H-252 in different phases of the culture cycle was studied. The presence of sodium chloride in the med...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Siberian Federal University
2011-09-01
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| Series: | Журнал Сибирского федерального университета: Серия Биология |
| Subjects: | |
| Online Access: | http://elib.sfu-kras.ru/bitstream/2311/2724/1/Zhila.pdf |
| Summary: | The effect of 0,3 and 0,7 M NaCl on biomass yield, total nitrogen content, intracellular lipid content, hydrocarbons and fatty acid profile of the lipids of the alga Botryococcus braunii Kütz IPPAS H-252 in different phases of the culture cycle was studied. The presence of sodium chloride in the medium inhibited the growth of algal cells for the first three days of the experiment, causing a decrease in total nitrogen, enhanced synthesis of triacylglycerols. In addition, considerable changes in the lipid fatty acid profile were found, i.e. decrease in polyunsaturated fatty acid (PUFA) levels (29,38 % and 12,8 %) and levels of long-chain saturated acids (5,3 % and 14,13 % of the total) at 0,3 M NaCl and 0,7 M NaCl, respectively. In later phases of the culture, at 0,3 M NaCl, the PUFA content increased up to the values characteristic of the active growth phase of the alga. At 0,7 M NaCl, the proportion of PUFA enhanced at less extent, but biomass concentration and total nitrogen increased, similarly to the experiment with 0,3 M NaCl, that may also be indicative of adaptation of the alga to the studied concentrations of NaCl. |
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| ISSN: | 1997-1389 2313-5530 |