Transcriptome and metabolome reveal the accumulation of secondary metabolites in different varieties of Cinnamomum longepaniculatum
Abstract Background Cinnamomum longepaniculatum (Gamble) N. Chao ex H. W. Li, whose leaves produce essential oils, is a traditional Chinese medicine and economically important tree species. In our study, two C. longepaniculatum varieties that have significantly different essential oil contents and l...
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-022-03637-2 |
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| author | Xin Zhao Yue Yan Wan-hai Zhou Rui-zhang Feng Yong-kang Shuai Li Yang Meng-jie Liu Xiu-yan He Qin Wei |
| author_facet | Xin Zhao Yue Yan Wan-hai Zhou Rui-zhang Feng Yong-kang Shuai Li Yang Meng-jie Liu Xiu-yan He Qin Wei |
| author_sort | Xin Zhao |
| collection | DOAJ |
| description | Abstract Background Cinnamomum longepaniculatum (Gamble) N. Chao ex H. W. Li, whose leaves produce essential oils, is a traditional Chinese medicine and economically important tree species. In our study, two C. longepaniculatum varieties that have significantly different essential oil contents and leaf phenotypes were selected as the materials to investigate secondary metabolism. Result The essential oil content and leaf phenotypes were different between the two varieties. When the results of both transcriptome and metabolomic analyses were combined, it was found that the differences were related to phenylalanine metabolic pathways, particularly the metabolism of flavonoids and terpenoids. The transcriptome results based on KEGG pathway enrichment analysis showed that pathways involving phenylpropanoids, tryptophan biosynthesis and terpenoids significantly differed between the two varieties; 11 DEGs (2 upregulated and 9 downregulated) were associated with the biosynthesis of other secondary metabolites, and 12 DEGs (2 upregulated and 10 downregulated) were related to the metabolism of terpenoids and polyketides. Through further analysis of the leaves, we detected 196 metabolites in C. longepaniculatum. The abundance of 49 (26 downregulated and 23 upregulated) metabolites differed between the two varieties, which is likely related to the differences in the accumulation of these metabolites. We identified 12 flavonoids, 8 terpenoids and 8 alkaloids and identified 4 kinds of PMFs from the leaves of C. longepaniculatum. Conclusions The combined results of transcriptome and metabolomic analyses revealed a strong correlation between metabolite contents and gene expression. We speculate that light leads to differences in the secondary metabolism and phenotypes of leaves of different varieties of C. longepaniculatum. This research provides data for secondary metabolite studies and lays a solid foundation for breeding ideal C. longepaniculatum plants. |
| first_indexed | 2024-04-13T18:52:10Z |
| format | Article |
| id | doaj.art-dadba7fe44f54e2798189d7ab4283ea1 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-13T18:52:10Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-dadba7fe44f54e2798189d7ab4283ea12022-12-22T02:34:24ZengBMCBMC Plant Biology1471-22292022-05-0122111410.1186/s12870-022-03637-2Transcriptome and metabolome reveal the accumulation of secondary metabolites in different varieties of Cinnamomum longepaniculatumXin Zhao0Yue Yan1Wan-hai Zhou2Rui-zhang Feng3Yong-kang Shuai4Li Yang5Meng-jie Liu6Xiu-yan He7Qin Wei8Faculty of Agriculture, Forestry and Food Engineering, YiBin UniversityFaculty of Agriculture, Forestry and Food Engineering, YiBin UniversityFaculty of Agriculture, Forestry and Food Engineering, YiBin UniversityFaculty of Agriculture, Forestry and Food Engineering, YiBin UniversityFaculty of Agriculture, Forestry and Food Engineering, YiBin UniversityFaculty of Agriculture, Forestry and Food Engineering, YiBin UniversityFaculty of Agriculture, Forestry and Food Engineering, YiBin UniversityFaculty of Agriculture, Forestry and Food Engineering, YiBin UniversityFaculty of Agriculture, Forestry and Food Engineering, YiBin UniversityAbstract Background Cinnamomum longepaniculatum (Gamble) N. Chao ex H. W. Li, whose leaves produce essential oils, is a traditional Chinese medicine and economically important tree species. In our study, two C. longepaniculatum varieties that have significantly different essential oil contents and leaf phenotypes were selected as the materials to investigate secondary metabolism. Result The essential oil content and leaf phenotypes were different between the two varieties. When the results of both transcriptome and metabolomic analyses were combined, it was found that the differences were related to phenylalanine metabolic pathways, particularly the metabolism of flavonoids and terpenoids. The transcriptome results based on KEGG pathway enrichment analysis showed that pathways involving phenylpropanoids, tryptophan biosynthesis and terpenoids significantly differed between the two varieties; 11 DEGs (2 upregulated and 9 downregulated) were associated with the biosynthesis of other secondary metabolites, and 12 DEGs (2 upregulated and 10 downregulated) were related to the metabolism of terpenoids and polyketides. Through further analysis of the leaves, we detected 196 metabolites in C. longepaniculatum. The abundance of 49 (26 downregulated and 23 upregulated) metabolites differed between the two varieties, which is likely related to the differences in the accumulation of these metabolites. We identified 12 flavonoids, 8 terpenoids and 8 alkaloids and identified 4 kinds of PMFs from the leaves of C. longepaniculatum. Conclusions The combined results of transcriptome and metabolomic analyses revealed a strong correlation between metabolite contents and gene expression. We speculate that light leads to differences in the secondary metabolism and phenotypes of leaves of different varieties of C. longepaniculatum. This research provides data for secondary metabolite studies and lays a solid foundation for breeding ideal C. longepaniculatum plants.https://doi.org/10.1186/s12870-022-03637-2Cinnamomum longepaniculatumSecondary metabolismFlavonoidsTerpenoids |
| spellingShingle | Xin Zhao Yue Yan Wan-hai Zhou Rui-zhang Feng Yong-kang Shuai Li Yang Meng-jie Liu Xiu-yan He Qin Wei Transcriptome and metabolome reveal the accumulation of secondary metabolites in different varieties of Cinnamomum longepaniculatum BMC Plant Biology Cinnamomum longepaniculatum Secondary metabolism Flavonoids Terpenoids |
| title | Transcriptome and metabolome reveal the accumulation of secondary metabolites in different varieties of Cinnamomum longepaniculatum |
| title_full | Transcriptome and metabolome reveal the accumulation of secondary metabolites in different varieties of Cinnamomum longepaniculatum |
| title_fullStr | Transcriptome and metabolome reveal the accumulation of secondary metabolites in different varieties of Cinnamomum longepaniculatum |
| title_full_unstemmed | Transcriptome and metabolome reveal the accumulation of secondary metabolites in different varieties of Cinnamomum longepaniculatum |
| title_short | Transcriptome and metabolome reveal the accumulation of secondary metabolites in different varieties of Cinnamomum longepaniculatum |
| title_sort | transcriptome and metabolome reveal the accumulation of secondary metabolites in different varieties of cinnamomum longepaniculatum |
| topic | Cinnamomum longepaniculatum Secondary metabolism Flavonoids Terpenoids |
| url | https://doi.org/10.1186/s12870-022-03637-2 |
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