Fecal Metaproteomics Reveals Reduced Gut Inflammation and Changed Microbial Metabolism Following Lifestyle-Induced Weight Loss
Gut microbiota-mediated inflammation promotes obesity-associated low-grade inflammation, which represents a hallmark of metabolic syndrome. To investigate if lifestyle-induced weight loss (WL) may modulate the gut microbiome composition and its interaction with the host on a functional level, we ana...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/5/726 |
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| author | Ronald Biemann Enrico Buß Dirk Benndorf Theresa Lehmann Kay Schallert Sebastian Püttker Udo Reichl Berend Isermann Jochen G. Schneider Gunter Saake Robert Heyer |
| author_facet | Ronald Biemann Enrico Buß Dirk Benndorf Theresa Lehmann Kay Schallert Sebastian Püttker Udo Reichl Berend Isermann Jochen G. Schneider Gunter Saake Robert Heyer |
| author_sort | Ronald Biemann |
| collection | DOAJ |
| description | Gut microbiota-mediated inflammation promotes obesity-associated low-grade inflammation, which represents a hallmark of metabolic syndrome. To investigate if lifestyle-induced weight loss (WL) may modulate the gut microbiome composition and its interaction with the host on a functional level, we analyzed the fecal metaproteome of 33 individuals with metabolic syndrome in a longitudinal study before and after lifestyle-induced WL in a well-defined cohort. The 6-month WL intervention resulted in reduced BMI (−13.7%), improved insulin sensitivity (HOMA-IR, −46.1%), and reduced levels of circulating hsCRP (−39.9%), indicating metabolic syndrome reversal. The metaprotein spectra revealed a decrease of human proteins associated with gut inflammation. Taxonomic analysis revealed only minor changes in the bacterial composition with an increase of the families Desulfovibrionaceae, Leptospiraceae, Syntrophomonadaceae, Thermotogaceae and Verrucomicrobiaceae. Yet we detected an increased abundance of microbial metaprotein spectra that suggest an enhanced hydrolysis of complex carbohydrates. Hence, lifestyle-induced WL was associated with reduced gut inflammation and functional changes of human and microbial enzymes for carbohydrate hydrolysis while the taxonomic composition of the gut microbiome remained almost stable. The metaproteomics workflow has proven to be a suitable method for monitoring inflammatory changes in the fecal metaproteome. |
| first_indexed | 2024-03-10T11:30:09Z |
| format | Article |
| id | doaj.art-dade76ade6da4dffa4198cb0f9ccc5a4 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T11:30:09Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-dade76ade6da4dffa4198cb0f9ccc5a42023-11-21T19:21:32ZengMDPI AGBiomolecules2218-273X2021-05-0111572610.3390/biom11050726Fecal Metaproteomics Reveals Reduced Gut Inflammation and Changed Microbial Metabolism Following Lifestyle-Induced Weight LossRonald Biemann0Enrico Buß1Dirk Benndorf2Theresa Lehmann3Kay Schallert4Sebastian Püttker5Udo Reichl6Berend Isermann7Jochen G. Schneider8Gunter Saake9Robert Heyer10Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, Leipzig University, Paul-List-Str. 13/15, 04103 Leipzig, GermanyBioprocess Engineering, Otto von Guericke University, Universitätsplatz 2, 39106 Magdeburg, GermanyBioprocess Engineering, Otto von Guericke University, Universitätsplatz 2, 39106 Magdeburg, GermanyBioprocess Engineering, Otto von Guericke University, Universitätsplatz 2, 39106 Magdeburg, GermanyBioprocess Engineering, Otto von Guericke University, Universitätsplatz 2, 39106 Magdeburg, GermanyBioprocess Engineering, Otto von Guericke University, Universitätsplatz 2, 39106 Magdeburg, GermanyBioprocess Engineering, Otto von Guericke University, Universitätsplatz 2, 39106 Magdeburg, GermanyInstitute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, Leipzig University, Paul-List-Str. 13/15, 04103 Leipzig, GermanyLuxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, 6, Avenue du Swing, L-4367 Belvaux, LuxembourgDatabase and Software Engineering Group, Otto von Guericke University, Universitätsplatz 2, 39106 Magdeburg, GermanyBioprocess Engineering, Otto von Guericke University, Universitätsplatz 2, 39106 Magdeburg, GermanyGut microbiota-mediated inflammation promotes obesity-associated low-grade inflammation, which represents a hallmark of metabolic syndrome. To investigate if lifestyle-induced weight loss (WL) may modulate the gut microbiome composition and its interaction with the host on a functional level, we analyzed the fecal metaproteome of 33 individuals with metabolic syndrome in a longitudinal study before and after lifestyle-induced WL in a well-defined cohort. The 6-month WL intervention resulted in reduced BMI (−13.7%), improved insulin sensitivity (HOMA-IR, −46.1%), and reduced levels of circulating hsCRP (−39.9%), indicating metabolic syndrome reversal. The metaprotein spectra revealed a decrease of human proteins associated with gut inflammation. Taxonomic analysis revealed only minor changes in the bacterial composition with an increase of the families Desulfovibrionaceae, Leptospiraceae, Syntrophomonadaceae, Thermotogaceae and Verrucomicrobiaceae. Yet we detected an increased abundance of microbial metaprotein spectra that suggest an enhanced hydrolysis of complex carbohydrates. Hence, lifestyle-induced WL was associated with reduced gut inflammation and functional changes of human and microbial enzymes for carbohydrate hydrolysis while the taxonomic composition of the gut microbiome remained almost stable. The metaproteomics workflow has proven to be a suitable method for monitoring inflammatory changes in the fecal metaproteome.https://www.mdpi.com/2218-273X/11/5/726metaproteomicsfecal samplesobesitymetabolic syndromegut inflammationmicrobiome |
| spellingShingle | Ronald Biemann Enrico Buß Dirk Benndorf Theresa Lehmann Kay Schallert Sebastian Püttker Udo Reichl Berend Isermann Jochen G. Schneider Gunter Saake Robert Heyer Fecal Metaproteomics Reveals Reduced Gut Inflammation and Changed Microbial Metabolism Following Lifestyle-Induced Weight Loss Biomolecules metaproteomics fecal samples obesity metabolic syndrome gut inflammation microbiome |
| title | Fecal Metaproteomics Reveals Reduced Gut Inflammation and Changed Microbial Metabolism Following Lifestyle-Induced Weight Loss |
| title_full | Fecal Metaproteomics Reveals Reduced Gut Inflammation and Changed Microbial Metabolism Following Lifestyle-Induced Weight Loss |
| title_fullStr | Fecal Metaproteomics Reveals Reduced Gut Inflammation and Changed Microbial Metabolism Following Lifestyle-Induced Weight Loss |
| title_full_unstemmed | Fecal Metaproteomics Reveals Reduced Gut Inflammation and Changed Microbial Metabolism Following Lifestyle-Induced Weight Loss |
| title_short | Fecal Metaproteomics Reveals Reduced Gut Inflammation and Changed Microbial Metabolism Following Lifestyle-Induced Weight Loss |
| title_sort | fecal metaproteomics reveals reduced gut inflammation and changed microbial metabolism following lifestyle induced weight loss |
| topic | metaproteomics fecal samples obesity metabolic syndrome gut inflammation microbiome |
| url | https://www.mdpi.com/2218-273X/11/5/726 |
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