Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture
The embryonic ontogeny of pineal secretory activity in birds has been investigated almost exclusively in chickens. This study aimed to characterize this process in domestic geese. The pineal organs of embryos aged 18–28 days were incubated in superfusion culture under different light conditions for...
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MDPI AG
2021-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/21/6329 |
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| author | Maria Hanuszewska-Dominiak Kamila Martyniuk Bogdan Lewczuk |
| author_facet | Maria Hanuszewska-Dominiak Kamila Martyniuk Bogdan Lewczuk |
| author_sort | Maria Hanuszewska-Dominiak |
| collection | DOAJ |
| description | The embryonic ontogeny of pineal secretory activity in birds has been investigated almost exclusively in chickens. This study aimed to characterize this process in domestic geese. The pineal organs of embryos aged 18–28 days were incubated in superfusion culture under different light conditions for 4–5 days and treated with norepinephrine (NE). Melatonin (MLT) was measured by radioimmunoassay and other indoles by HPLC with fluorescence detection. Additionally, pineal organs were collected from embryos at 14–28 days of age and used to measure catecholamines by HPLC with electrochemical detection. MLT secretion increased with embryo age, most intensively between the 22nd and 24th days of life. The daily changes in MLT secretion under the 12 L:12D cycle occurred on the first day of culture, starting from an embryonic age of 24 days. MLT secretion was controlled by the light-dark cycle in all age groups studied. However, exposure to light during the scotophase did not alter the secretion of MLT. The endogenous oscillator expressed its activity in regulating MLT secretion in the pineal organs of embryos aged 24 days and older but could not generate a rhythm after one cycle. The rhythm of 5-hydroxytryptophan release during the first day of culture was found in the pineal organs of all embryos, while the rhythmic release of <i>N</i>-acetylserotonin and 5-methoxyindole acetic acid started at the age of 24 days. The proportion of released indoles changed with embryo age. NE caused a decrease in MLT secretion and provoked an increase in serotonin release. Incubation of the pineal organs induced the development of MLT secretory machinery and its diurnal rhythmicity. The pineal content of catecholamines increased prominently at the end of embryonic development. |
| first_indexed | 2024-03-10T05:57:15Z |
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| id | doaj.art-dae146ec85e44beb96dd4ad3685c7174 |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:57:15Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-dae146ec85e44beb96dd4ad3685c71742023-11-22T21:19:38ZengMDPI AGMolecules1420-30492021-10-012621632910.3390/molecules26216329Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion CultureMaria Hanuszewska-Dominiak0Kamila Martyniuk1Bogdan Lewczuk2Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, PolandDepartment of Histology and Embryology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, PolandDepartment of Histology and Embryology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, PolandThe embryonic ontogeny of pineal secretory activity in birds has been investigated almost exclusively in chickens. This study aimed to characterize this process in domestic geese. The pineal organs of embryos aged 18–28 days were incubated in superfusion culture under different light conditions for 4–5 days and treated with norepinephrine (NE). Melatonin (MLT) was measured by radioimmunoassay and other indoles by HPLC with fluorescence detection. Additionally, pineal organs were collected from embryos at 14–28 days of age and used to measure catecholamines by HPLC with electrochemical detection. MLT secretion increased with embryo age, most intensively between the 22nd and 24th days of life. The daily changes in MLT secretion under the 12 L:12D cycle occurred on the first day of culture, starting from an embryonic age of 24 days. MLT secretion was controlled by the light-dark cycle in all age groups studied. However, exposure to light during the scotophase did not alter the secretion of MLT. The endogenous oscillator expressed its activity in regulating MLT secretion in the pineal organs of embryos aged 24 days and older but could not generate a rhythm after one cycle. The rhythm of 5-hydroxytryptophan release during the first day of culture was found in the pineal organs of all embryos, while the rhythmic release of <i>N</i>-acetylserotonin and 5-methoxyindole acetic acid started at the age of 24 days. The proportion of released indoles changed with embryo age. NE caused a decrease in MLT secretion and provoked an increase in serotonin release. Incubation of the pineal organs induced the development of MLT secretory machinery and its diurnal rhythmicity. The pineal content of catecholamines increased prominently at the end of embryonic development.https://www.mdpi.com/1420-3049/26/21/6329pineal organmelatoninindolesembryoontogenynorepinephrine |
| spellingShingle | Maria Hanuszewska-Dominiak Kamila Martyniuk Bogdan Lewczuk Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture Molecules pineal organ melatonin indoles embryo ontogeny norepinephrine |
| title | Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture |
| title_full | Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture |
| title_fullStr | Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture |
| title_full_unstemmed | Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture |
| title_short | Embryonic Development of Avian Pineal Secretory Activity—A Lesson from the Goose Pineal Organs in Superfusion Culture |
| title_sort | embryonic development of avian pineal secretory activity a lesson from the goose pineal organs in superfusion culture |
| topic | pineal organ melatonin indoles embryo ontogeny norepinephrine |
| url | https://www.mdpi.com/1420-3049/26/21/6329 |
| work_keys_str_mv | AT mariahanuszewskadominiak embryonicdevelopmentofavianpinealsecretoryactivityalessonfromthegoosepinealorgansinsuperfusionculture AT kamilamartyniuk embryonicdevelopmentofavianpinealsecretoryactivityalessonfromthegoosepinealorgansinsuperfusionculture AT bogdanlewczuk embryonicdevelopmentofavianpinealsecretoryactivityalessonfromthegoosepinealorgansinsuperfusionculture |