GENETIC SURVEY OF INVASIVE S. PNEUMONIAE SEROTYPES IN BULGARIA FOR A 5-YEAR PERIOD
Streptococcus pneumoniae colonises the mucosal lining of the upper respiratory tract and is an important cause of invasive infections affecting young children, adults over 65 years of age, the immunocompromised and individuals with chronic diseases. Recent studies have shown variations in virulence...
| Үндсэн зохиолчид: | , , , |
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| Формат: | Өгүүллэг |
| Хэл сонгох: | English |
| Хэвлэсэн: |
National Center of Infectious and Parasitic Diseases
2018-12-01
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| Цуврал: | Problems of Infectious and Parasitic Diseases |
| Нөхцлүүд: | |
| Онлайн хандалт: | https://pipd.ncipd.org/index.php/pipd/article/view/17 |
| _version_ | 1827946668037767168 |
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| author | Ivan Simeonovski Viktoriya Levterova Mariya Malcheva Todor Kantardjiev |
| author_facet | Ivan Simeonovski Viktoriya Levterova Mariya Malcheva Todor Kantardjiev |
| author_sort | Ivan Simeonovski |
| collection | DOAJ |
| description |
Streptococcus pneumoniae colonises the mucosal lining of the upper respiratory tract and is an important cause of invasive infections affecting young children, adults over 65 years of age, the immunocompromised and individuals with chronic diseases. Recent studies have shown variations in virulence based on the high rate of pneumococcal recombination. PCR-based molecular methods are highly sensitive, specific and are becoming the preferred tool for quick and accurate diagnosis of bacterial meningitis which is required to be defined within 2-3 hours.
During the 5-year survey period (2013-2017), 202 materials received as cerebrospinal fluid samples and pneumococcal strains isolated from patients diagnosed with meningitis, were examined by Real-time PCR in the reference laboratory at NCIPD. Serotyping of S. pneumoniae-positive materials was performed with conventional multiplex PCR and Real-time PCR with primers for 41serotypes/serogroups.
There is a high incidence of S. pneumoniae serotypes not covered by the pneumococcal conjugate vaccine (PCV10) currently used in Bulgaria. It was found that all cases of meningitis caused by S. pneumoniae vaccine serotypes occurred in patients that were not vaccinated.
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| first_indexed | 2024-03-13T11:04:51Z |
| format | Article |
| id | doaj.art-dae2e86a6f95464b8f67f9dd2889373f |
| institution | Directory Open Access Journal |
| issn | 0204-9155 2815-2808 |
| language | English |
| last_indexed | 2024-03-13T11:04:51Z |
| publishDate | 2018-12-01 |
| publisher | National Center of Infectious and Parasitic Diseases |
| record_format | Article |
| series | Problems of Infectious and Parasitic Diseases |
| spelling | doaj.art-dae2e86a6f95464b8f67f9dd2889373f2023-05-16T09:17:25ZengNational Center of Infectious and Parasitic DiseasesProblems of Infectious and Parasitic Diseases0204-91552815-28082018-12-0146210.58395/pipd.v46i2.17GENETIC SURVEY OF INVASIVE S. PNEUMONIAE SEROTYPES IN BULGARIA FOR A 5-YEAR PERIODIvan Simeonovski0Viktoriya Levterova1Mariya Malcheva2Todor Kantardjiev3National Centre of Infectious and Parasitic DiseasesNational Centre of Infectious and Parasitic DiseasesNational Centre of Infectious and Parasitic DiseasesNational Centre of Infectious and Parasitic Diseases Streptococcus pneumoniae colonises the mucosal lining of the upper respiratory tract and is an important cause of invasive infections affecting young children, adults over 65 years of age, the immunocompromised and individuals with chronic diseases. Recent studies have shown variations in virulence based on the high rate of pneumococcal recombination. PCR-based molecular methods are highly sensitive, specific and are becoming the preferred tool for quick and accurate diagnosis of bacterial meningitis which is required to be defined within 2-3 hours. During the 5-year survey period (2013-2017), 202 materials received as cerebrospinal fluid samples and pneumococcal strains isolated from patients diagnosed with meningitis, were examined by Real-time PCR in the reference laboratory at NCIPD. Serotyping of S. pneumoniae-positive materials was performed with conventional multiplex PCR and Real-time PCR with primers for 41serotypes/serogroups. There is a high incidence of S. pneumoniae serotypes not covered by the pneumococcal conjugate vaccine (PCV10) currently used in Bulgaria. It was found that all cases of meningitis caused by S. pneumoniae vaccine serotypes occurred in patients that were not vaccinated. https://pipd.ncipd.org/index.php/pipd/article/view/17serotypinginvasive S. pneumoniaePCR |
| spellingShingle | Ivan Simeonovski Viktoriya Levterova Mariya Malcheva Todor Kantardjiev GENETIC SURVEY OF INVASIVE S. PNEUMONIAE SEROTYPES IN BULGARIA FOR A 5-YEAR PERIOD Problems of Infectious and Parasitic Diseases serotyping invasive S. pneumoniae PCR |
| title | GENETIC SURVEY OF INVASIVE S. PNEUMONIAE SEROTYPES IN BULGARIA FOR A 5-YEAR PERIOD |
| title_full | GENETIC SURVEY OF INVASIVE S. PNEUMONIAE SEROTYPES IN BULGARIA FOR A 5-YEAR PERIOD |
| title_fullStr | GENETIC SURVEY OF INVASIVE S. PNEUMONIAE SEROTYPES IN BULGARIA FOR A 5-YEAR PERIOD |
| title_full_unstemmed | GENETIC SURVEY OF INVASIVE S. PNEUMONIAE SEROTYPES IN BULGARIA FOR A 5-YEAR PERIOD |
| title_short | GENETIC SURVEY OF INVASIVE S. PNEUMONIAE SEROTYPES IN BULGARIA FOR A 5-YEAR PERIOD |
| title_sort | genetic survey of invasive s pneumoniae serotypes in bulgaria for a 5 year period |
| topic | serotyping invasive S. pneumoniae PCR |
| url | https://pipd.ncipd.org/index.php/pipd/article/view/17 |
| work_keys_str_mv | AT ivansimeonovski geneticsurveyofinvasivespneumoniaeserotypesinbulgariafora5yearperiod AT viktoriyalevterova geneticsurveyofinvasivespneumoniaeserotypesinbulgariafora5yearperiod AT mariyamalcheva geneticsurveyofinvasivespneumoniaeserotypesinbulgariafora5yearperiod AT todorkantardjiev geneticsurveyofinvasivespneumoniaeserotypesinbulgariafora5yearperiod |