Identification of novel elements of the Drosophila blisterome sheds light on potential pathological mechanisms of several human diseases.
Main developmental programs are highly conserved among species of the animal kingdom. Improper execution of these programs often leads to progression of various diseases and disorders. Here we focused on Drosophila wing tissue morphogenesis, a fairly complex developmental program, one of the steps o...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4072764?pdf=render |
| _version_ | 1818261547065016320 |
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| author | Oleksii Bilousov Alexey Koval Amiran Keshelava Vladimir L Katanaev |
| author_facet | Oleksii Bilousov Alexey Koval Amiran Keshelava Vladimir L Katanaev |
| author_sort | Oleksii Bilousov |
| collection | DOAJ |
| description | Main developmental programs are highly conserved among species of the animal kingdom. Improper execution of these programs often leads to progression of various diseases and disorders. Here we focused on Drosophila wing tissue morphogenesis, a fairly complex developmental program, one of the steps of which--apposition of the dorsal and ventral wing sheets during metamorphosis--is mediated by integrins. Disruption of this apposition leads to wing blistering which serves as an easily screenable phenotype for components regulating this process. By means of RNAi-silencing technique and the blister phenotype as readout, we identify numerous novel proteins potentially involved in wing sheet adhesion. Remarkably, our results reveal not only participants of the integrin-mediated machinery, but also components of other cellular processes, e.g. cell cycle, RNA splicing, and vesicular trafficking. With the use of bioinformatics tools, these data are assembled into a large blisterome network. Analysis of human orthologues of the Drosophila blisterome components shows that many disease-related genes may contribute to cell adhesion implementation, providing hints on possible mechanisms of these human pathologies. |
| first_indexed | 2024-12-12T18:48:57Z |
| format | Article |
| id | doaj.art-dae44ba8c5274a5889ac89bc74a52e4c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T18:48:57Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-dae44ba8c5274a5889ac89bc74a52e4c2022-12-22T00:15:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e10113310.1371/journal.pone.0101133Identification of novel elements of the Drosophila blisterome sheds light on potential pathological mechanisms of several human diseases.Oleksii BilousovAlexey KovalAmiran KeshelavaVladimir L KatanaevMain developmental programs are highly conserved among species of the animal kingdom. Improper execution of these programs often leads to progression of various diseases and disorders. Here we focused on Drosophila wing tissue morphogenesis, a fairly complex developmental program, one of the steps of which--apposition of the dorsal and ventral wing sheets during metamorphosis--is mediated by integrins. Disruption of this apposition leads to wing blistering which serves as an easily screenable phenotype for components regulating this process. By means of RNAi-silencing technique and the blister phenotype as readout, we identify numerous novel proteins potentially involved in wing sheet adhesion. Remarkably, our results reveal not only participants of the integrin-mediated machinery, but also components of other cellular processes, e.g. cell cycle, RNA splicing, and vesicular trafficking. With the use of bioinformatics tools, these data are assembled into a large blisterome network. Analysis of human orthologues of the Drosophila blisterome components shows that many disease-related genes may contribute to cell adhesion implementation, providing hints on possible mechanisms of these human pathologies.http://europepmc.org/articles/PMC4072764?pdf=render |
| spellingShingle | Oleksii Bilousov Alexey Koval Amiran Keshelava Vladimir L Katanaev Identification of novel elements of the Drosophila blisterome sheds light on potential pathological mechanisms of several human diseases. PLoS ONE |
| title | Identification of novel elements of the Drosophila blisterome sheds light on potential pathological mechanisms of several human diseases. |
| title_full | Identification of novel elements of the Drosophila blisterome sheds light on potential pathological mechanisms of several human diseases. |
| title_fullStr | Identification of novel elements of the Drosophila blisterome sheds light on potential pathological mechanisms of several human diseases. |
| title_full_unstemmed | Identification of novel elements of the Drosophila blisterome sheds light on potential pathological mechanisms of several human diseases. |
| title_short | Identification of novel elements of the Drosophila blisterome sheds light on potential pathological mechanisms of several human diseases. |
| title_sort | identification of novel elements of the drosophila blisterome sheds light on potential pathological mechanisms of several human diseases |
| url | http://europepmc.org/articles/PMC4072764?pdf=render |
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