Global mapping of translation initiation sites by TIS profiling in budding yeast

Global mapping of translation initiation sites by TIS profiling in budding yeast

Summary: Translation initiation site (TIS) profiling allows for the genome-wide identification of TISs in vivo by exclusively capturing mRNA fragments within ribosomes that have just completed translation initiation. It leverages translation inhibitors, such as harringtonine and lactimidomycin (LTM)...

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Bibliographic Details
Main Authors:	Ina Hollerer, Emily N. Powers, Gloria A. Brar
Format:	Article
Language:	English
Published:	Elsevier 2021-03-01
Series:	STAR Protocols
Subjects:	Cell biology Genomics High-throughput screening Model organisms Molecular biology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166720302379

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