The full recovery of mice (Mus Musculus C57BL/6 strain) from virus–induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAs
Background: Virus-induced cellular genetic modifications result in the development of many human cancers. Methods: In our experiments, we used the RVP3 cell line, which produce primary mouse virus-induced sarcoma in 100% of cases. Inbreed 4-week-old female C57BL/6 mice were injected subcutaneously i...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2019-06-01
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| Series: | Non-coding RNA Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054018301045 |
| _version_ | 1797205252038983680 |
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| author | Oxana V. Klimenko Alexey Sidorov |
| author_facet | Oxana V. Klimenko Alexey Sidorov |
| author_sort | Oxana V. Klimenko |
| collection | DOAJ |
| description | Background: Virus-induced cellular genetic modifications result in the development of many human cancers. Methods: In our experiments, we used the RVP3 cell line, which produce primary mouse virus-induced sarcoma in 100% of cases. Inbreed 4-week-old female C57BL/6 mice were injected subcutaneously in the interscapular region with RVP3 cells. Three groups of mice were used. For treatment, one and/or two intravenous injections of a complex of small non-coding RNAs (sncRNAs) a-miR-155, piR-30074, and miR-125b with a 2-diethylaminoethyl-dextran methyl methacrylate copolymer (DDMC) delivery system were used. The first group consisted of untreated animals (control). The second group was treated with one injection of complex DDMC/sncRNAs (1st group). The third group was treated with two injections of complex DDMC/sncRNAs (2nd group). The tumors were removed aseptically, freed of necrotic material, and used with spleen and lungs for subsequent RT-PCR and immunofluorescence experiments, or stained with Leishman-Romanowski dye. Results: As a result, the mice fully recovered from virus-induced sarcoma after two treatments with a complex including the DDMC vector and a-miR-155, piR-30074, and miR-125b. In vitro studies showed genetic and morphological transformations of murine cancer cells after the injections. Conclusions: Treatment of virus-induced sarcoma of mice with a-miR-155, piR-30074, and miR-125b as active component of anti-cancer complex and DDMC vector as delivery system due to epigenetic-regulated transformation of cancer cells into cells with non-cancerous physiology and morphology and full recovery of disease. Keywords: Small non-coding RNAs, Src tyrosine kinase, Epigenetic therapy, Mice, DDMC vector, Sarcoma |
| first_indexed | 2024-04-24T08:48:10Z |
| format | Article |
| id | doaj.art-daf3578e692349f789e50a5e2fb6c55c |
| institution | Directory Open Access Journal |
| issn | 2468-0540 |
| language | English |
| last_indexed | 2024-04-24T08:48:10Z |
| publishDate | 2019-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Non-coding RNA Research |
| spelling | doaj.art-daf3578e692349f789e50a5e2fb6c55c2024-04-16T12:59:24ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402019-06-01426978The full recovery of mice (Mus Musculus C57BL/6 strain) from virus–induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAsOxana V. Klimenko0Alexey Sidorov1Corresponding author.; SID ALEX GROUP, Ltd., Kyselova 1185/2, Prague, 182 00, Czech RepublicSID ALEX GROUP, Ltd., Kyselova 1185/2, Prague, 182 00, Czech RepublicBackground: Virus-induced cellular genetic modifications result in the development of many human cancers. Methods: In our experiments, we used the RVP3 cell line, which produce primary mouse virus-induced sarcoma in 100% of cases. Inbreed 4-week-old female C57BL/6 mice were injected subcutaneously in the interscapular region with RVP3 cells. Three groups of mice were used. For treatment, one and/or two intravenous injections of a complex of small non-coding RNAs (sncRNAs) a-miR-155, piR-30074, and miR-125b with a 2-diethylaminoethyl-dextran methyl methacrylate copolymer (DDMC) delivery system were used. The first group consisted of untreated animals (control). The second group was treated with one injection of complex DDMC/sncRNAs (1st group). The third group was treated with two injections of complex DDMC/sncRNAs (2nd group). The tumors were removed aseptically, freed of necrotic material, and used with spleen and lungs for subsequent RT-PCR and immunofluorescence experiments, or stained with Leishman-Romanowski dye. Results: As a result, the mice fully recovered from virus-induced sarcoma after two treatments with a complex including the DDMC vector and a-miR-155, piR-30074, and miR-125b. In vitro studies showed genetic and morphological transformations of murine cancer cells after the injections. Conclusions: Treatment of virus-induced sarcoma of mice with a-miR-155, piR-30074, and miR-125b as active component of anti-cancer complex and DDMC vector as delivery system due to epigenetic-regulated transformation of cancer cells into cells with non-cancerous physiology and morphology and full recovery of disease. Keywords: Small non-coding RNAs, Src tyrosine kinase, Epigenetic therapy, Mice, DDMC vector, Sarcomahttp://www.sciencedirect.com/science/article/pii/S2468054018301045 |
| spellingShingle | Oxana V. Klimenko Alexey Sidorov The full recovery of mice (Mus Musculus C57BL/6 strain) from virus–induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAs Non-coding RNA Research |
| title | The full recovery of mice (Mus Musculus C57BL/6 strain) from virus–induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAs |
| title_full | The full recovery of mice (Mus Musculus C57BL/6 strain) from virus–induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAs |
| title_fullStr | The full recovery of mice (Mus Musculus C57BL/6 strain) from virus–induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAs |
| title_full_unstemmed | The full recovery of mice (Mus Musculus C57BL/6 strain) from virus–induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAs |
| title_short | The full recovery of mice (Mus Musculus C57BL/6 strain) from virus–induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAs |
| title_sort | full recovery of mice mus musculus c57bl 6 strain from virus induced sarcoma after treatment with a complex of ddmc delivery system and sncrnas |
| url | http://www.sciencedirect.com/science/article/pii/S2468054018301045 |
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