Subregion-specific modulation of excitatory input and dopaminergic output in the striatum by tonically activated glycine and GABAA receptors
The flow of cortical information through the basal ganglia is a complex spatiotemporal pattern of increased and decreased firing. The striatum is the biggest input nucleus to the basal ganglia and the aim of this study was to assess the role of inhibitory GABAA and glycine receptors in regulating sy...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2011-10-01
|
| Series: | Frontiers in Systems Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnsys.2011.00085/full |
| _version_ | 1818578122391420928 |
|---|---|
| author | Louise eAdermark Rhona B Clarke Mia eEricson Bo eSöderpalm |
| author_facet | Louise eAdermark Rhona B Clarke Mia eEricson Bo eSöderpalm |
| author_sort | Louise eAdermark |
| collection | DOAJ |
| description | The flow of cortical information through the basal ganglia is a complex spatiotemporal pattern of increased and decreased firing. The striatum is the biggest input nucleus to the basal ganglia and the aim of this study was to assess the role of inhibitory GABAA and glycine receptors in regulating synaptic activity in the dorsolateral (DLS) and ventral striatum (nucleus accumbens, nAc). Local field potential recordings from coronal brain slices of juvenile and adult Wistar rats showed that GABAA receptors and strychnine-sensitive glycine receptors are tonically activated and inhibit excitatory input to the DLS and to the nAc. Strychnine-induced disinhibition of glutamatergic transmission was insensitive to the muscarinic receptor inhibitor scopolamine (10 µM), inhibited by the nicotinic acetylcholine receptor antagonist mecamylamine (10 µM) and blocked by GABAA receptor inhibitors, suggesting that tonically activated glycine receptors depress excitatory input to the striatum through modulation of cholinergic and GABAergic neurotransmission. As an end-product example of striatal GABAergic output in vivo we measured dopamine release in the DLS and nAc by microdialysis in the awake and freely moving rat. Reversed dialysis of bicuculline (50 μM in perfusate) only increased extrasynaptic dopamine levels in the nAc, while strychnine administered locally (200 μM in perfusate) decreased dopamine output by 60% in both the DLS and nAc. Our data suggest that GABAA and glycine receptors are tonically activated and modulate striatal transmission in a partially sub-region specific manner. |
| first_indexed | 2024-12-16T06:40:47Z |
| format | Article |
| id | doaj.art-daf3cdb9c7a543cfa6fc6447b015c75d |
| institution | Directory Open Access Journal |
| issn | 1662-5137 |
| language | English |
| last_indexed | 2024-12-16T06:40:47Z |
| publishDate | 2011-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Systems Neuroscience |
| spelling | doaj.art-daf3cdb9c7a543cfa6fc6447b015c75d2022-12-21T22:40:42ZengFrontiers Media S.A.Frontiers in Systems Neuroscience1662-51372011-10-01510.3389/fnsys.2011.0008513753Subregion-specific modulation of excitatory input and dopaminergic output in the striatum by tonically activated glycine and GABAA receptorsLouise eAdermark0Rhona B Clarke1Mia eEricson2Bo eSöderpalm3University of GothenburgUniversity of GothenburgUniversity of GothenburgUniversity of GothenburgThe flow of cortical information through the basal ganglia is a complex spatiotemporal pattern of increased and decreased firing. The striatum is the biggest input nucleus to the basal ganglia and the aim of this study was to assess the role of inhibitory GABAA and glycine receptors in regulating synaptic activity in the dorsolateral (DLS) and ventral striatum (nucleus accumbens, nAc). Local field potential recordings from coronal brain slices of juvenile and adult Wistar rats showed that GABAA receptors and strychnine-sensitive glycine receptors are tonically activated and inhibit excitatory input to the DLS and to the nAc. Strychnine-induced disinhibition of glutamatergic transmission was insensitive to the muscarinic receptor inhibitor scopolamine (10 µM), inhibited by the nicotinic acetylcholine receptor antagonist mecamylamine (10 µM) and blocked by GABAA receptor inhibitors, suggesting that tonically activated glycine receptors depress excitatory input to the striatum through modulation of cholinergic and GABAergic neurotransmission. As an end-product example of striatal GABAergic output in vivo we measured dopamine release in the DLS and nAc by microdialysis in the awake and freely moving rat. Reversed dialysis of bicuculline (50 μM in perfusate) only increased extrasynaptic dopamine levels in the nAc, while strychnine administered locally (200 μM in perfusate) decreased dopamine output by 60% in both the DLS and nAc. Our data suggest that GABAA and glycine receptors are tonically activated and modulate striatal transmission in a partially sub-region specific manner.http://journal.frontiersin.org/Journal/10.3389/fnsys.2011.00085/fullBasal GangliaBicucullineNucleus AccumbensStrychnineratdorsolateral striatum |
| spellingShingle | Louise eAdermark Rhona B Clarke Mia eEricson Bo eSöderpalm Subregion-specific modulation of excitatory input and dopaminergic output in the striatum by tonically activated glycine and GABAA receptors Frontiers in Systems Neuroscience Basal Ganglia Bicuculline Nucleus Accumbens Strychnine rat dorsolateral striatum |
| title | Subregion-specific modulation of excitatory input and dopaminergic output in the striatum by tonically activated glycine and GABAA receptors |
| title_full | Subregion-specific modulation of excitatory input and dopaminergic output in the striatum by tonically activated glycine and GABAA receptors |
| title_fullStr | Subregion-specific modulation of excitatory input and dopaminergic output in the striatum by tonically activated glycine and GABAA receptors |
| title_full_unstemmed | Subregion-specific modulation of excitatory input and dopaminergic output in the striatum by tonically activated glycine and GABAA receptors |
| title_short | Subregion-specific modulation of excitatory input and dopaminergic output in the striatum by tonically activated glycine and GABAA receptors |
| title_sort | subregion specific modulation of excitatory input and dopaminergic output in the striatum by tonically activated glycine and gabaa receptors |
| topic | Basal Ganglia Bicuculline Nucleus Accumbens Strychnine rat dorsolateral striatum |
| url | http://journal.frontiersin.org/Journal/10.3389/fnsys.2011.00085/full |
| work_keys_str_mv | AT louiseeadermark subregionspecificmodulationofexcitatoryinputanddopaminergicoutputinthestriatumbytonicallyactivatedglycineandgabaareceptors AT rhonabclarke subregionspecificmodulationofexcitatoryinputanddopaminergicoutputinthestriatumbytonicallyactivatedglycineandgabaareceptors AT miaeericson subregionspecificmodulationofexcitatoryinputanddopaminergicoutputinthestriatumbytonicallyactivatedglycineandgabaareceptors AT boesoderpalm subregionspecificmodulationofexcitatoryinputanddopaminergicoutputinthestriatumbytonicallyactivatedglycineandgabaareceptors |