Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future Perspectives
Mono- and polyunsaturated lipids are particularly susceptible to peroxidation, which results in the formation of lipid hydroperoxides (LOOHs) as primary nonradical-reaction products. LOOHs may undergo degradation to various products that have been implicated in vital biological reactions, and thus i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/7/2139 |
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| author | Vassiliki G. Kontogianni Ioannis P. Gerothanassis |
| author_facet | Vassiliki G. Kontogianni Ioannis P. Gerothanassis |
| author_sort | Vassiliki G. Kontogianni |
| collection | DOAJ |
| description | Mono- and polyunsaturated lipids are particularly susceptible to peroxidation, which results in the formation of lipid hydroperoxides (LOOHs) as primary nonradical-reaction products. LOOHs may undergo degradation to various products that have been implicated in vital biological reactions, and thus in the pathogenesis of various diseases. The structure elucidation and qualitative and quantitative analysis of lipid hydroperoxides are therefore of great importance. The objectives of the present review are to provide a critical analysis of various methods that have been widely applied, and more specifically on volumetric methods, applications of UV-visible, infrared, Raman/surface-enhanced Raman, fluorescence and chemiluminescence spectroscopies, chromatographic methods, hyphenated MS techniques, NMR and chromatographic methods, NMR spectroscopy in mixture analysis, structural investigations based on quantum chemical calculations of NMR parameters, applications in living cells, and metabolomics. Emphasis will be given to analytical and structural methods that can contribute significantly to the molecular basis of the chemical process involved in the formation of lipid hydroperoxides without the need for the isolation of the individual components. Furthermore, future developments in the field will be discussed. |
| first_indexed | 2024-03-09T11:37:34Z |
| format | Article |
| id | doaj.art-daf573454e1b4fa0aa8b39ada7f55f17 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T11:37:34Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-daf573454e1b4fa0aa8b39ada7f55f172023-11-30T23:40:05ZengMDPI AGMolecules1420-30492022-03-01277213910.3390/molecules27072139Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future PerspectivesVassiliki G. Kontogianni0Ioannis P. Gerothanassis1Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, GR-45110 Ioannina, GreeceSection of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, GR-45110 Ioannina, GreeceMono- and polyunsaturated lipids are particularly susceptible to peroxidation, which results in the formation of lipid hydroperoxides (LOOHs) as primary nonradical-reaction products. LOOHs may undergo degradation to various products that have been implicated in vital biological reactions, and thus in the pathogenesis of various diseases. The structure elucidation and qualitative and quantitative analysis of lipid hydroperoxides are therefore of great importance. The objectives of the present review are to provide a critical analysis of various methods that have been widely applied, and more specifically on volumetric methods, applications of UV-visible, infrared, Raman/surface-enhanced Raman, fluorescence and chemiluminescence spectroscopies, chromatographic methods, hyphenated MS techniques, NMR and chromatographic methods, NMR spectroscopy in mixture analysis, structural investigations based on quantum chemical calculations of NMR parameters, applications in living cells, and metabolomics. Emphasis will be given to analytical and structural methods that can contribute significantly to the molecular basis of the chemical process involved in the formation of lipid hydroperoxides without the need for the isolation of the individual components. Furthermore, future developments in the field will be discussed.https://www.mdpi.com/1420-3049/27/7/2139lipidshydroperoxidesHPLCMSNMRIR |
| spellingShingle | Vassiliki G. Kontogianni Ioannis P. Gerothanassis Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future Perspectives Molecules lipids hydroperoxides HPLC MS NMR IR |
| title | Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future Perspectives |
| title_full | Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future Perspectives |
| title_fullStr | Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future Perspectives |
| title_full_unstemmed | Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future Perspectives |
| title_short | Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future Perspectives |
| title_sort | analytical and structural tools of lipid hydroperoxides present state and future perspectives |
| topic | lipids hydroperoxides HPLC MS NMR IR |
| url | https://www.mdpi.com/1420-3049/27/7/2139 |
| work_keys_str_mv | AT vassilikigkontogianni analyticalandstructuraltoolsoflipidhydroperoxidespresentstateandfutureperspectives AT ioannispgerothanassis analyticalandstructuraltoolsoflipidhydroperoxidespresentstateandfutureperspectives |