Interaction of nitrobenzoxadiazole derivatives of piperazine and aniline with bovine serum albumine in silico and in vitro
Albumin is a globular protein of plasma of mammalian blood participating in transport of hydrophobic metabolites and drugs. Thus, studies devoted to its binding are valuable as a part of evaluation of new potential drugs or fluorescent probes for in vivo usage. Here we describe results concerning sy...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Belarusian |
| Published: |
Belarusian State University
2021-09-01
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| Series: | Журнал Белорусского государственного университета: Химия |
| Subjects: | |
| Online Access: | https://journals.bsu.by/index.php/chemistry/article/view/4099 |
| Summary: | Albumin is a globular protein of plasma of mammalian blood participating in transport of hydrophobic metabolites and drugs. Thus, studies devoted to its binding are valuable as a part of evaluation of new potential drugs or fluorescent probes for in vivo usage. Here we describe results concerning synthesis and bovine serum albumin binding assay both in silico (docking) and in vitro (spectrophotometric and spectrofluorimetric titrations) for four new 7-nitrobenzoxadiazol-4-yl (NBD) derivatives of aniline and piperazine. Experimental dissociation constant for NBD-ethynylaniline 4 was calculated to be about 10 µmol/L. |
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| ISSN: | 2520-257X 2617-3980 |