Nano-Curcumin/Chitosan Modulates Growth, Biochemical, Immune, and Antioxidative Profiles, and the Expression of Related Genes in <i>Nile tilapia</i>, <i>Oreochromis niloticus</i>
This study aimed to evaluate the effects of dietary supplementation with nano-curcumin (NCur) and a nano-curcumin/chitosan blend (NCur/Ch) on growth performance, digestibility, immune response, antioxidant status, intestinal morphometric characters, and gene regulation in <i>Nile tilapia</i...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/8/7/333 |
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| author | Hiam Elabd Heba H. Mahboub Shimaa M. R. Salem Abdelwahab M. Abdelwahab Khairiah Mubarak Alwutayd Mohamed Shaalan Sameh H. Ismail Abdelfattah M. Abdelfattah A. Khalid Abdallah Tageldein Mansour Heba S. Hamed Hadeer Youssuf |
| author_facet | Hiam Elabd Heba H. Mahboub Shimaa M. R. Salem Abdelwahab M. Abdelwahab Khairiah Mubarak Alwutayd Mohamed Shaalan Sameh H. Ismail Abdelfattah M. Abdelfattah A. Khalid Abdallah Tageldein Mansour Heba S. Hamed Hadeer Youssuf |
| author_sort | Hiam Elabd |
| collection | DOAJ |
| description | This study aimed to evaluate the effects of dietary supplementation with nano-curcumin (NCur) and a nano-curcumin/chitosan blend (NCur/Ch) on growth performance, digestibility, immune response, antioxidant status, intestinal morphometric characters, and gene regulation in <i>Nile tilapia</i>. Fish (n = 180, initial body weight = 12.0 ± 0.53 g) received supplementary NCur at rates of 0 (control), 0.00625, and 0.0125, and NCur/Ch at rates of 0.00625 + 0.5 g/kg diet for 4 weeks. Growth performance parameters (final weight and length, body mass gain, specific growth, and length gain rates) were markedly increased, and the feed conversion ratio was significantly decreased in the NCur- and NCur/Ch-supplemented groups. Digestive enzyme (amylase), immune response markers (immunoglobulin M, nitrous oxide, and lysozyme activity), plasma albumin, and total protein were increased significantly, mainly with a diet supplemented with 0.00625 g NCur/kg. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), glucose, and cortisol levels decreased in the supplemented groups compared to the control. Significantly increased glutathione peroxidase (GPx) and decreased malondialdehyde (MDA) levels were observed in the NCur/Ch group. Superoxide dismutase (SOD) activity was improved in the 0.0125 NCur group. Intestinal morphometric characters, including villus length, width, interspace, and goblet cell abundance, were increased to cope with improved growth performance and were associated with upregulation of insulin-like growth factor 1 (<i>igf-1</i>) and complement C-5 (<i>cc5</i>) compared to the control group. Therefore, NCur and an NCur/Ch blend could be supplemented in the <i>Nile tilapia</i> diets as a natural alternative to promote growth, digestion, immune status, liver function, antioxidant status, and related gene expression in <i>O. niloticus</i>. |
| first_indexed | 2024-03-11T01:06:07Z |
| format | Article |
| id | doaj.art-dafcf521b6414fa283f5bed0b81671dd |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-11T01:06:07Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj.art-dafcf521b6414fa283f5bed0b81671dd2023-11-18T19:17:51ZengMDPI AGFishes2410-38882023-06-018733310.3390/fishes8070333Nano-Curcumin/Chitosan Modulates Growth, Biochemical, Immune, and Antioxidative Profiles, and the Expression of Related Genes in <i>Nile tilapia</i>, <i>Oreochromis niloticus</i>Hiam Elabd0Heba H. Mahboub1Shimaa M. R. Salem2Abdelwahab M. Abdelwahab3Khairiah Mubarak Alwutayd4Mohamed Shaalan5Sameh H. Ismail6Abdelfattah M. Abdelfattah7A. Khalid8Abdallah Tageldein Mansour9Heba S. Hamed10Hadeer Youssuf11Department of Aquatic Animals’ Medicine, Faculty of Veterinary Medicine, Benha University, Moshtohor 13736, EgyptDepartment of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptDepartment of Animal Nutrition and Nutritional Deficiency Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Animal Production, Faculty of Agriculture, Fayoum University, Fayoum 63514, EgyptDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza 12613, EgyptFaculty of Nanotechnology for Postgraduate Studies, Sheikh Zayed Branch Campus, Cairo University, Sheikh Zayed City 12588, EgyptDepartment of Clinical Pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, EgyptDepartment of Basic Engineering Sciences, Faculty of Engineering (Shoubra), Benha University, Banha 11637, EgyptAnimal and Fish Production Department, College of Agricultural and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi ArabiaDepartment of Zoology, Faculty of Women for Arts, Science & Education, Ain Sham University, Cairo 11757, EgyptDepartment of Aquatic Animals’ Medicine, Faculty of Veterinary Medicine, Benha University, Moshtohor 13736, EgyptThis study aimed to evaluate the effects of dietary supplementation with nano-curcumin (NCur) and a nano-curcumin/chitosan blend (NCur/Ch) on growth performance, digestibility, immune response, antioxidant status, intestinal morphometric characters, and gene regulation in <i>Nile tilapia</i>. Fish (n = 180, initial body weight = 12.0 ± 0.53 g) received supplementary NCur at rates of 0 (control), 0.00625, and 0.0125, and NCur/Ch at rates of 0.00625 + 0.5 g/kg diet for 4 weeks. Growth performance parameters (final weight and length, body mass gain, specific growth, and length gain rates) were markedly increased, and the feed conversion ratio was significantly decreased in the NCur- and NCur/Ch-supplemented groups. Digestive enzyme (amylase), immune response markers (immunoglobulin M, nitrous oxide, and lysozyme activity), plasma albumin, and total protein were increased significantly, mainly with a diet supplemented with 0.00625 g NCur/kg. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), glucose, and cortisol levels decreased in the supplemented groups compared to the control. Significantly increased glutathione peroxidase (GPx) and decreased malondialdehyde (MDA) levels were observed in the NCur/Ch group. Superoxide dismutase (SOD) activity was improved in the 0.0125 NCur group. Intestinal morphometric characters, including villus length, width, interspace, and goblet cell abundance, were increased to cope with improved growth performance and were associated with upregulation of insulin-like growth factor 1 (<i>igf-1</i>) and complement C-5 (<i>cc5</i>) compared to the control group. Therefore, NCur and an NCur/Ch blend could be supplemented in the <i>Nile tilapia</i> diets as a natural alternative to promote growth, digestion, immune status, liver function, antioxidant status, and related gene expression in <i>O. niloticus</i>.https://www.mdpi.com/2410-3888/8/7/333nano-curcuminchitosanimmune-antioxidant profilehistopathologygene expression |
| spellingShingle | Hiam Elabd Heba H. Mahboub Shimaa M. R. Salem Abdelwahab M. Abdelwahab Khairiah Mubarak Alwutayd Mohamed Shaalan Sameh H. Ismail Abdelfattah M. Abdelfattah A. Khalid Abdallah Tageldein Mansour Heba S. Hamed Hadeer Youssuf Nano-Curcumin/Chitosan Modulates Growth, Biochemical, Immune, and Antioxidative Profiles, and the Expression of Related Genes in <i>Nile tilapia</i>, <i>Oreochromis niloticus</i> Fishes nano-curcumin chitosan immune-antioxidant profile histopathology gene expression |
| title | Nano-Curcumin/Chitosan Modulates Growth, Biochemical, Immune, and Antioxidative Profiles, and the Expression of Related Genes in <i>Nile tilapia</i>, <i>Oreochromis niloticus</i> |
| title_full | Nano-Curcumin/Chitosan Modulates Growth, Biochemical, Immune, and Antioxidative Profiles, and the Expression of Related Genes in <i>Nile tilapia</i>, <i>Oreochromis niloticus</i> |
| title_fullStr | Nano-Curcumin/Chitosan Modulates Growth, Biochemical, Immune, and Antioxidative Profiles, and the Expression of Related Genes in <i>Nile tilapia</i>, <i>Oreochromis niloticus</i> |
| title_full_unstemmed | Nano-Curcumin/Chitosan Modulates Growth, Biochemical, Immune, and Antioxidative Profiles, and the Expression of Related Genes in <i>Nile tilapia</i>, <i>Oreochromis niloticus</i> |
| title_short | Nano-Curcumin/Chitosan Modulates Growth, Biochemical, Immune, and Antioxidative Profiles, and the Expression of Related Genes in <i>Nile tilapia</i>, <i>Oreochromis niloticus</i> |
| title_sort | nano curcumin chitosan modulates growth biochemical immune and antioxidative profiles and the expression of related genes in i nile tilapia i i oreochromis niloticus i |
| topic | nano-curcumin chitosan immune-antioxidant profile histopathology gene expression |
| url | https://www.mdpi.com/2410-3888/8/7/333 |
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