The generation of free radicals in the development of grey mould on poinsettia leaves
Participation of superoxide anion and hydroxyl radical as well as lipoxygenase (LOX) activity and chemiluminescence in the development of grey mould on poinsettia leaves of the cvs.: Coco White - moderately resistant and Malibu Red - susceptible to Botryotinia fuckeliana (B. cinerea) were investigat...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2013-12-01
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| Series: | Acta Agrobotanica |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/aa/article/view/2543 |
| _version_ | 1818323077818220544 |
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| author | Beata Kułek Jolanta Florczyk-Wieczorek Zbigniew Górski |
| author_facet | Beata Kułek Jolanta Florczyk-Wieczorek Zbigniew Górski |
| author_sort | Beata Kułek |
| collection | DOAJ |
| description | Participation of superoxide anion and hydroxyl radical as well as lipoxygenase (LOX) activity and chemiluminescence in the development of grey mould on poinsettia leaves of the cvs.: Coco White - moderately resistant and Malibu Red - susceptible to Botryotinia fuckeliana (B. cinerea) were investigated. In both poinsettia cultivars, the level of superoxide anion radical increased with the disease development. On later dates following inoculation, the content of O2.- in the diseased leaf areas of the susceptible cultivar was 2 - fold larger than in grey mould symptom areas of the moderately resistant cultivar. The postinfection generation of a strongly reactive hydroxyl radical was found in the cultivar susceptible to the pathogen. The level of .- OH in the last hours afier inoculation was 10 fold higher in grey mould spots than in healthy senescing leaves of that cultivar. Tlie activity of LOX exhibited opposite tendencies of changes in the both genotypes examined after the infection. In the first hours after inoculation, the enzyme activity was Low, whereas after 3rt day it significantly increased in diseased leaf areas and in their surroundings. However, in the moderately resistant cultivar the activity of lipoxygenase thoughout the first 3 days of the disease activity was relatively high and then decreased. Poinsettia leaves infected with B.cinerea emitted chemiluminescence. Kinetics and the intensity of ultraweak chemiluminescence depended on the cultivars of poinsettia and on the degree of the disease development. The postinfection increase of chemiluminescence was particularly visible in the zone surrounding diseased areas in the susceptible cultivar. |
| first_indexed | 2024-12-13T11:06:58Z |
| format | Article |
| id | doaj.art-db02a469d3fe44e892c2fe7e551e7438 |
| institution | Directory Open Access Journal |
| issn | 2300-357X |
| language | English |
| last_indexed | 2024-12-13T11:06:58Z |
| publishDate | 2013-12-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Agrobotanica |
| spelling | doaj.art-db02a469d3fe44e892c2fe7e551e74382022-12-21T23:48:57ZengPolish Botanical SocietyActa Agrobotanica2300-357X2013-12-0155115717110.5586/aa.2002.0162060The generation of free radicals in the development of grey mould on poinsettia leavesBeata Kułek0Jolanta Florczyk-Wieczorek1Zbigniew Górski2Agricultural University of August CieszkowskiAgricultural University of August CieszkowskiPoznań University of TechnologyParticipation of superoxide anion and hydroxyl radical as well as lipoxygenase (LOX) activity and chemiluminescence in the development of grey mould on poinsettia leaves of the cvs.: Coco White - moderately resistant and Malibu Red - susceptible to Botryotinia fuckeliana (B. cinerea) were investigated. In both poinsettia cultivars, the level of superoxide anion radical increased with the disease development. On later dates following inoculation, the content of O2.- in the diseased leaf areas of the susceptible cultivar was 2 - fold larger than in grey mould symptom areas of the moderately resistant cultivar. The postinfection generation of a strongly reactive hydroxyl radical was found in the cultivar susceptible to the pathogen. The level of .- OH in the last hours afier inoculation was 10 fold higher in grey mould spots than in healthy senescing leaves of that cultivar. Tlie activity of LOX exhibited opposite tendencies of changes in the both genotypes examined after the infection. In the first hours after inoculation, the enzyme activity was Low, whereas after 3rt day it significantly increased in diseased leaf areas and in their surroundings. However, in the moderately resistant cultivar the activity of lipoxygenase thoughout the first 3 days of the disease activity was relatively high and then decreased. Poinsettia leaves infected with B.cinerea emitted chemiluminescence. Kinetics and the intensity of ultraweak chemiluminescence depended on the cultivars of poinsettia and on the degree of the disease development. The postinfection increase of chemiluminescence was particularly visible in the zone surrounding diseased areas in the susceptible cultivar.https://pbsociety.org.pl/journals/index.php/aa/article/view/2543active oxygen speciesultra weak chemiluminescencegrey mouldimaginglipoxygenasepoinsettia |
| spellingShingle | Beata Kułek Jolanta Florczyk-Wieczorek Zbigniew Górski The generation of free radicals in the development of grey mould on poinsettia leaves Acta Agrobotanica active oxygen species ultra weak chemiluminescence grey mould imaging lipoxygenase poinsettia |
| title | The generation of free radicals in the development of grey mould on poinsettia leaves |
| title_full | The generation of free radicals in the development of grey mould on poinsettia leaves |
| title_fullStr | The generation of free radicals in the development of grey mould on poinsettia leaves |
| title_full_unstemmed | The generation of free radicals in the development of grey mould on poinsettia leaves |
| title_short | The generation of free radicals in the development of grey mould on poinsettia leaves |
| title_sort | generation of free radicals in the development of grey mould on poinsettia leaves |
| topic | active oxygen species ultra weak chemiluminescence grey mould imaging lipoxygenase poinsettia |
| url | https://pbsociety.org.pl/journals/index.php/aa/article/view/2543 |
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