Chemical and microbiological characterization of Cambisols, Luvisols and Stagnosols
Cambisols, Luvisols and Stagnosols are main soil taxonomical units covering 78% of the total area of the Czech Republic. In the period 2001-2008 soil samples from 13 arable and 2 grassed localities were collected and tested. Microbial biomass carbon (MBC) was determined using microwave irradiation m...
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| Format: | Article |
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Czech Academy of Agricultural Sciences
2009-06-01
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| Series: | Plant, Soil and Environment |
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| Online Access: | https://pse.agriculturejournals.cz/artkey/pse-200906-0002_chemical-and-microbiological-characterization-of-cambisols-luvisols-and-stagnosols.php |
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| author | L. Růžek M. Růžková K. Voříšek J. Kubát M. Friedlová O. Mikanová |
| author_facet | L. Růžek M. Růžková K. Voříšek J. Kubát M. Friedlová O. Mikanová |
| author_sort | L. Růžek |
| collection | DOAJ |
| description | Cambisols, Luvisols and Stagnosols are main soil taxonomical units covering 78% of the total area of the Czech Republic. In the period 2001-2008 soil samples from 13 arable and 2 grassed localities were collected and tested. Microbial biomass carbon (MBC) was determined using microwave irradiation method (MW), chloroform fumigation extraction method (FE) and re-hydration technique (RHD). Soil samples were moistened, according to the content of organic carbon (Corg), by deionized water addition (%Corg × 0.19 ml/g DM - dry mass of soil) immediately before MBC determination. Microwave sterilization (800 J/g DM = 600 W, 2 × 67 s, 100 g DM (10 soil samples) and microwave soil extracts digestion (800 J/ml = 250 W, 77 s, 24 ml) give the lowest values of MBC (204 ± 67 mg/kg DM; 100%) in comparison with FE (236 ± 57 mg/kg DM; 116%) and RHD (478 ± 138 mg/kg DM; 235%), respectively. High significant correlation (r = 0.9713) was found between TC (total carbon; 1.36 ± 0.29%) and TN (total nitrogen; 0.15 ± 0.03%) determined by CNS analyzer. Furthermore, high significant correlations were found between MBC-MW and MBC-RHD (r = 0.8965) as well as MBC-FE and DHA (dehydrogenase activity; r = 0.8094), respectively. DHA in studied soils reached 147 ± 68 mg of triphenylformazan/kg DM/24 h. Corg formed 96% of TC and total Kjeldahl nitrogen 97% of TN, respectively. According our results MW is fully acceptable for MBC determination. |
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| issn | 1214-1178 1805-9368 |
| language | English |
| last_indexed | 2024-04-10T08:12:29Z |
| publishDate | 2009-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
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| series | Plant, Soil and Environment |
| spelling | doaj.art-db02ffe68a5641d09c69f194ccba876d2023-02-23T03:45:31ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682009-06-0155623123710.17221/35/2009-PSEpse-200906-0002Chemical and microbiological characterization of Cambisols, Luvisols and StagnosolsL. Růžek0M. Růžková1K. Voříšek2J. Kubát3M. Friedlová4O. Mikanová5Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech RepublicFaculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech RepublicFaculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech RepublicCrop Research Institute, Prague, Czech RepublicCrop Research Institute, Prague, Czech RepublicCrop Research Institute, Prague, Czech RepublicCambisols, Luvisols and Stagnosols are main soil taxonomical units covering 78% of the total area of the Czech Republic. In the period 2001-2008 soil samples from 13 arable and 2 grassed localities were collected and tested. Microbial biomass carbon (MBC) was determined using microwave irradiation method (MW), chloroform fumigation extraction method (FE) and re-hydration technique (RHD). Soil samples were moistened, according to the content of organic carbon (Corg), by deionized water addition (%Corg × 0.19 ml/g DM - dry mass of soil) immediately before MBC determination. Microwave sterilization (800 J/g DM = 600 W, 2 × 67 s, 100 g DM (10 soil samples) and microwave soil extracts digestion (800 J/ml = 250 W, 77 s, 24 ml) give the lowest values of MBC (204 ± 67 mg/kg DM; 100%) in comparison with FE (236 ± 57 mg/kg DM; 116%) and RHD (478 ± 138 mg/kg DM; 235%), respectively. High significant correlation (r = 0.9713) was found between TC (total carbon; 1.36 ± 0.29%) and TN (total nitrogen; 0.15 ± 0.03%) determined by CNS analyzer. Furthermore, high significant correlations were found between MBC-MW and MBC-RHD (r = 0.8965) as well as MBC-FE and DHA (dehydrogenase activity; r = 0.8094), respectively. DHA in studied soils reached 147 ± 68 mg of triphenylformazan/kg DM/24 h. Corg formed 96% of TC and total Kjeldahl nitrogen 97% of TN, respectively. According our results MW is fully acceptable for MBC determination.https://pse.agriculturejournals.cz/artkey/pse-200906-0002_chemical-and-microbiological-characterization-of-cambisols-luvisols-and-stagnosols.phpmicrobial biomasstotal carbon and nitrogenk2so4 extractable carbondehydrogenasecambisolsluvisolsinceptisolsalfisols |
| spellingShingle | L. Růžek M. Růžková K. Voříšek J. Kubát M. Friedlová O. Mikanová Chemical and microbiological characterization of Cambisols, Luvisols and Stagnosols Plant, Soil and Environment microbial biomass total carbon and nitrogen k2so4 extractable carbon dehydrogenase cambisols luvisols inceptisols alfisols |
| title | Chemical and microbiological characterization of Cambisols, Luvisols and Stagnosols |
| title_full | Chemical and microbiological characterization of Cambisols, Luvisols and Stagnosols |
| title_fullStr | Chemical and microbiological characterization of Cambisols, Luvisols and Stagnosols |
| title_full_unstemmed | Chemical and microbiological characterization of Cambisols, Luvisols and Stagnosols |
| title_short | Chemical and microbiological characterization of Cambisols, Luvisols and Stagnosols |
| title_sort | chemical and microbiological characterization of cambisols luvisols and stagnosols |
| topic | microbial biomass total carbon and nitrogen k2so4 extractable carbon dehydrogenase cambisols luvisols inceptisols alfisols |
| url | https://pse.agriculturejournals.cz/artkey/pse-200906-0002_chemical-and-microbiological-characterization-of-cambisols-luvisols-and-stagnosols.php |
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