Brassinosteroid Accelerates Wound Healing of Potato Tubers by Activation of Reactive Oxygen Metabolism and Phenylpropanoid Metabolism
Wound healing could effectively reduce the decay rate of potato tubers after harvest, but it took a long time to form typical and complete healing structures. Brassinosteroid (BR), as a sterol hormone, is important for enhancing plant resistance to abiotic and biotic stresses. However, it has not be...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/7/906 |
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| author | Ye Han Ruirui Yang Xuejiao Zhang Qihui Wang Bin Wang Xiaoyuan Zheng Yongcai Li Dov Prusky Yang Bi |
| author_facet | Ye Han Ruirui Yang Xuejiao Zhang Qihui Wang Bin Wang Xiaoyuan Zheng Yongcai Li Dov Prusky Yang Bi |
| author_sort | Ye Han |
| collection | DOAJ |
| description | Wound healing could effectively reduce the decay rate of potato tubers after harvest, but it took a long time to form typical and complete healing structures. Brassinosteroid (BR), as a sterol hormone, is important for enhancing plant resistance to abiotic and biotic stresses. However, it has not been reported that if BR affects wound healing of potato tubers. In the present study, we observed that BR played a positive role in the accumulation of lignin and suberin polyphenolic (SPP) at the wounds, and effectively reduced the weight loss and disease index of potato tubers (cv. Atlantic) during healing. At the end of healing, the weight loss and disease index of BR group was 30.8% and 23.1% lower than the control, respectively. Furthermore, BR activated the expression of <i>StPAL</i>, <i>St4CL</i>, <i>StCAD</i> genes and related enzyme activities in phenylpropanoid metabolism, and promoted the synthesis of lignin precursors and phenolic acids at the wound site, mainly by inducing the synthesis of caffeic acid, sinapic acid and cinnamyl alcohol. Meanwhile, the expression of <i>StNOX</i> was induced and the production of O<sup>2−</sup> and H<sub>2</sub>O<sub>2</sub> was promoted, which mediated oxidative crosslinking of above phenolic acids and lignin precursors to form SPP and lignin. In addition, the expression level of <i>StPOD</i> was partially increased. In contrast, the inhibitor brassinazole inhibited phenylpropanoid metabolism and reactive oxygen metabolism, and demonstrated the function of BR hormone in healing in reverse. Taken together, the activation of reactive oxygen metabolism and phenylpropanoid metabolism by BR could accelerate the wound healing of potato tubers. |
| first_indexed | 2024-03-09T11:51:54Z |
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| language | English |
| last_indexed | 2024-03-09T11:51:54Z |
| publishDate | 2022-03-01 |
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| series | Foods |
| spelling | doaj.art-db091f4f523549ddbc2a022961d231c62023-11-30T23:14:16ZengMDPI AGFoods2304-81582022-03-0111790610.3390/foods11070906Brassinosteroid Accelerates Wound Healing of Potato Tubers by Activation of Reactive Oxygen Metabolism and Phenylpropanoid MetabolismYe Han0Ruirui Yang1Xuejiao Zhang2Qihui Wang3Bin Wang4Xiaoyuan Zheng5Yongcai Li6Dov Prusky7Yang Bi8College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaDepartment of Postharvest Science of Fresh Produce, Agricultural Research Organization, Rishon LeZion 7505101, IsraelCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaWound healing could effectively reduce the decay rate of potato tubers after harvest, but it took a long time to form typical and complete healing structures. Brassinosteroid (BR), as a sterol hormone, is important for enhancing plant resistance to abiotic and biotic stresses. However, it has not been reported that if BR affects wound healing of potato tubers. In the present study, we observed that BR played a positive role in the accumulation of lignin and suberin polyphenolic (SPP) at the wounds, and effectively reduced the weight loss and disease index of potato tubers (cv. Atlantic) during healing. At the end of healing, the weight loss and disease index of BR group was 30.8% and 23.1% lower than the control, respectively. Furthermore, BR activated the expression of <i>StPAL</i>, <i>St4CL</i>, <i>StCAD</i> genes and related enzyme activities in phenylpropanoid metabolism, and promoted the synthesis of lignin precursors and phenolic acids at the wound site, mainly by inducing the synthesis of caffeic acid, sinapic acid and cinnamyl alcohol. Meanwhile, the expression of <i>StNOX</i> was induced and the production of O<sup>2−</sup> and H<sub>2</sub>O<sub>2</sub> was promoted, which mediated oxidative crosslinking of above phenolic acids and lignin precursors to form SPP and lignin. In addition, the expression level of <i>StPOD</i> was partially increased. In contrast, the inhibitor brassinazole inhibited phenylpropanoid metabolism and reactive oxygen metabolism, and demonstrated the function of BR hormone in healing in reverse. Taken together, the activation of reactive oxygen metabolism and phenylpropanoid metabolism by BR could accelerate the wound healing of potato tubers.https://www.mdpi.com/2304-8158/11/7/906wound healingpotato tubersphenylpropanoid metabolismROSbrassinosteroidabiotic stress |
| spellingShingle | Ye Han Ruirui Yang Xuejiao Zhang Qihui Wang Bin Wang Xiaoyuan Zheng Yongcai Li Dov Prusky Yang Bi Brassinosteroid Accelerates Wound Healing of Potato Tubers by Activation of Reactive Oxygen Metabolism and Phenylpropanoid Metabolism Foods wound healing potato tubers phenylpropanoid metabolism ROS brassinosteroid abiotic stress |
| title | Brassinosteroid Accelerates Wound Healing of Potato Tubers by Activation of Reactive Oxygen Metabolism and Phenylpropanoid Metabolism |
| title_full | Brassinosteroid Accelerates Wound Healing of Potato Tubers by Activation of Reactive Oxygen Metabolism and Phenylpropanoid Metabolism |
| title_fullStr | Brassinosteroid Accelerates Wound Healing of Potato Tubers by Activation of Reactive Oxygen Metabolism and Phenylpropanoid Metabolism |
| title_full_unstemmed | Brassinosteroid Accelerates Wound Healing of Potato Tubers by Activation of Reactive Oxygen Metabolism and Phenylpropanoid Metabolism |
| title_short | Brassinosteroid Accelerates Wound Healing of Potato Tubers by Activation of Reactive Oxygen Metabolism and Phenylpropanoid Metabolism |
| title_sort | brassinosteroid accelerates wound healing of potato tubers by activation of reactive oxygen metabolism and phenylpropanoid metabolism |
| topic | wound healing potato tubers phenylpropanoid metabolism ROS brassinosteroid abiotic stress |
| url | https://www.mdpi.com/2304-8158/11/7/906 |
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